Indophenol blue colorimetric method for measuring cation exchange capacity in sandy soils

Santoni, Stefania; Bonifacio, Eleonora; Zanini, Ermanno

doi:10.1081/css-120000388

Cited by 27 publications

(10 citation statements)

References 8 publications

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“…Nitrate content was calculated using a calibration curve prepared with KNO 3 and expressed in mg NO 3 - ⋅g -1 Fw. Ammonium content was measured using the method of indophenol blue colorimetry at 630 nm (Zanini, 2001). Ammonium chloride was used as standard.…”

Section: Methodsmentioning

confidence: 99%

Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels

et al. 2017

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To investigate the role of nitrogen (N) metabolism in the adaptation of photosynthesis to water stress in rice, a hydroponic experiment supplying with low N (0.72 mM), moderate N (2.86 mM), and high N (7.15 mM) followed by 150 g⋅L-1 PEG-6000 induced water stress was conducted in a rainout shelter. Water stress induced stomatal limitation to photosynthesis at low N, but no significant effect was observed at moderate and high N. Non-photochemical quenching was higher at moderate and high N. In contrast, relative excessive energy at PSII level (EXC) was declined with increasing N level. Malondialdehyde and hydrogen peroxide (H2O2) contents were in parallel with EXC. Water stress decreased catalase and ascorbate peroxidase activities at low N, resulting in increased H2O2 content and severer membrane lipid peroxidation; whereas the activities of antioxidative enzymes were increased at high N. In accordance with photosynthetic rate and antioxidative enzymes, water stress decreased the activities of key enzymes involving in N metabolism such as glutamate synthase and glutamate dehydrogenase, and photorespiratory key enzyme glycolate oxidase at low N. Concurrently, water stress increased nitrate content significantly at low N, but decreased nitrate content at moderate and high N. Contrary to nitrate, water stress increased proline content at moderate and high N. Our results suggest that N metabolism appears to be associated with the tolerance of photosynthesis to water stress in rice via affecting CO2 diffusion, antioxidant capacity, and osmotic adjustment.

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Section: Methodsmentioning

confidence: 99%

Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels

et al. 2017

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“…To determine the content of NH 4 + , shoots and roots were sampled as described above and extracted with deionized water for 30 min. NH 4 + content was measured using indophenol blue colorimetry at an absorbance wavelength of 630 nm [67].…”

Section: Determination Of Nh 4 + Contentmentioning

confidence: 99%

Ammonium Accumulation Caused by Reduced Tonoplast V-ATPase Activity in Arabidopsis thaliana

Liang

Song

Xiao

et al. 2020

IJMS

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Plant vacuoles are unique compartments that play a critical role in plant growth and development. The vacuolar H+-ATPase (V-ATPase), together with the vacuolar H+-pyrophosphatase (V-PPase), generates the proton motive force that regulates multiple cell functions and impacts all aspects of plant life. We investigated the effect of V-ATPase activity in the vacuole on plant growth and development. We used an Arabidopsisthaliana (L.) Heynh. double mutant, vha-a2 vha-a3, which lacks two tonoplast-localized isoforms of the membrane-integral V-ATPase subunit VHA-a. The mutant is viable but exhibits impaired growth and leaf chlorosis. Nitrate assimilation led to excessive ammonium accumulation in the shoot and lower nitrogen uptake, which exacerbated growth retardation of vha-a2 vha-a3. Ion homeostasis was disturbed in plants with missing VHA-a2 and VHA-a3 genes, which might be related to limited growth. The reduced growth and excessive ammonium accumulation of the double mutant was alleviated by potassium supplementation. Our results demonstrate that plants lacking the two tonoplast-localized subunits of V-ATPase can be viable, although with defective growth caused by multiple factors, which can be alleviated by adding potassium. This study provided a new insight into the relationship between V-ATPase, growth, and ammonium accumulation, and revealed the role of potassium in mitigating ammonium toxicity.

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“…To determine the concentration of NH 4 + , shoots and roots were sampled as described above and extracted with deionized water for 30 min. NH 4 + concentration was measured using indophenol blue colorimetry at 630 nm [41].…”

Section: Measurement Of Nh 4 + Concentrationmentioning

confidence: 99%

“…The xylem sap was collected from 20 plants and the total volume was approximately 50 µL for each replicate [48]. Ammonium was determined as described by Santoni et al [41].…”

Section: Determination Of Ammonium In Xylem Sapmentioning

confidence: 99%

Growth Retardation Caused by Reduced Tonoplast V-ATPase Activity in Arabidopsis thaliana

Liang¹,

Liu²,

Xiao³

et al. 2020

Preprint

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Background: Vacuoles are unique compartments in plant cells. Their multiple functions require massive fluxes of molecules across their limiting membrane, the tonoplast. Transport across the tonoplast is energized by its membrane potential and the proton gradient established by two proton pumps, the vacuolar H+-ATPase (V-ATPase), and the vacuolar H+-pyrophosphatase (V-PPase), which play important roles in the growth and development of plants. However, the mechanisms by which they affect plant growth and development, in the absence of tonoplast proton pumps, remain unclear. Results: In this study, we show that the Arabidopsis thaliana double mutant, vha-a2vha-a3, which lacks two tonoplast-localized isoforms of the membrane-integral V-ATPase subunit VHA-a, is viable, but shows a phenotype of inhibited growth and leaf chlorosis. Reduced total nitrogen absorption and increased nitrate assimilation lead to the accumulation of ammonium in the shoot, which results in the growth retardation of vha-a2vha-a3. Concurrently, the distribution and concentration of cations are abnormal in plants when VHA-a2 and VHA-a3 genes are absent, which is another major growth-limiting factor. Interestingly, the growth inhibition of the vha-a2vha-a3 double mutant was alleviated and senescence delayed, while the concentration of total nitrogen increased and that of ammonium decreased, when adding additional potassium. Conclusions: Our results show that plants can be viable without VHA-a2 and VHA-a3 but are affected by multiple factors that result in growth retardation, which can be alleviated by adding additional potassium, which provides a new insight on the relationship between vacuolar H+-ATPase and potassium.

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Indophenol blue colorimetric method for measuring cation exchange capacity in sandy soils

Cited by 27 publications

References 8 publications

Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels

Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels

Ammonium Accumulation Caused by Reduced Tonoplast V-ATPase Activity in Arabidopsis thaliana

Growth Retardation Caused by Reduced Tonoplast V-ATPase Activity in Arabidopsis thaliana

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