Indomethacin Prevents Neuronal Apoptosis in Newborn Rats with Hypoxic-Ischemic Brain Injury

Abstract

A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Cyclo oxy ge na se path way and pros tag lan dins play an im por tant ro le in the pat ho ge ne sis and de la yed mec ha nisms of hypo xic-isc he mic bra in in jury. The aim of this study was to in ves ti ga te the ef fect of dif fe rent do ses of in do met ha cin, a non se lec ti ve cyclo oxy ge na se in hi bitor, on ne u ro nal apop to sis in rats with hypo xic-isc he mic bra in in jury. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : Seven-day-old rat pups with the Ri ce mo del of hypo xic-isc he mic ce reb ral in jury we re ran domly di vi ded in to fi ve gro ups. Gro up 1 (n= 15) pups we re gi ven physi o lo gic sa li ne, ne it her li ga ti on nor hypo xi a we re per for med. Gro up 2 (n= 15) pups we re tre a ted with physi o lo gic sa li ne af ter hypoxic-isc he mi a. Gro up 3 (n= 15) pups we re tre a ted with in do met ha cin at a do se of 2 mg/kg be fo re hypo xic isc he mi a. Gro up 4 (n= 15) pups we re tre a ted with thre e do ses of in do met ha cin at a dose of 2 mg/kg every 12 h af ter hypo xic-isc he mi a. Gro up 5 (n= 15) pups we re tre a ted with thre e do ses of in do met ha cin, at a do se of 4mg/kg every 12 h af ter hypo xic isc he mi a. Af ter 72 ho urs, the rats we re de ca pi ta ted and bra in he misp he res we re eva lu a ted by the TU NEL (Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling) sta i ning met hod. R Re e s su ul lt ts s: : In do met ha cin tre at ment, eit her be fo re or af ter hypo xi a, re sul ted in a sig ni fi cant re duc ti on in the num bers of apopto tic cells in the rat bra in when com pa red to tho se who we re tre a ted with physi o lo gic sa li ne af ter hypo xic-isc he mi a (P< 0.001). C Co on nc c l lu u s si i o on n: : Our re sults de mons tra ted that in do met ha cin ad mi nistra ti on, eit her be fo re or af ter hypo xic-isc he mi a, re du ces ne u ro nal apop to sis; and we pro po se that in do met ha cin may be a po ten ti al cho i ce of tre at ment for hypo xic-isc he mic bra in in jury.K Ke ey y W Wo or rd ds s: : Hypo xi a-isc he mi a, bra in; apop to sis; in do met ha cin Ö ÖZ ZE ET T A Am ma aç ç: : Sik lo ok si je naz yo la ğı ve pros tag lan din ler, hi pok sik-is ke mik be yin ha sa rı nın pa to gene zin de ve geç dö nem ki sü re cin de önem li rol oy na mak ta dır. Bu ça lış ma nın ama cı, bir non-se lektif sik lo ok si je naz in hi bi tö rü olan in do me ta zi ni fark lı doz lar da uy gu la rak hi pok sik-is ke mik be yin ha sar lı rat lar da nö ro nal apo pi to zis üze ri ne et ki le ri ni araş tır mak tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Le vi neRi ce me to du na gö re hi pok sik is ke mi oluş tu ru lan ye di gün lük rat yav ru la rı rast ge le beş gru ba ay rıl -dı. Grup 1'de ki (n: 15) yav ru la ra ne li gas yon ne de hi pok si uy gu lan ma dan sa de ce se rum fiz yo lo jik ve ril di. Grup 2'de ki (n: 15) yav ru la ra hi pok sik-is ke mik ha le ge ti ril dik ten son ra se rum fiz yo lo jik veril di. Grup 3'te ki (n: 15) yav...