Indolent T‐cell‐rich small B‐cell hepatic lymphoma in a Golden Retriever

Rout, Emily D.; Hughes, Ken; Boostrom, Brendan O.; Seelig, Davis; Avery, Anne C.; Avery, P.

doi:10.1002/ccr3.1580

Cited by 3 publications

(3 citation statements)

References 53 publications

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“…In these cases, histopathology and immunohistochemistry remain mandatory. Additionally, PCR for Antigen Receptor Rearrangements (PARR) analysis can help in achieving the correct diagnosis and define lineage allocation ( 20 ); however it can be less sensitive and accurate than FC to predict B and T phenotype ( 10 ).…”

Section: Differentiating Reactive and Neoplastic B-cellsmentioning

confidence: 99%

Flow Cytometry in the Diagnosis of Canine B-Cell Lymphoma

Riondato

Comazzi

2021

Front. Vet. Sci.

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B cell lymphoma (BCL) is a heterogeneous group of lymphoid malignancies which comprise the majority of canine lymphomas. Diffuse large B cell lymphoma is the most common lymphoma subtype in dogs but other subtypes (e.g., marginal zone lymphoma, follicular lymphoma, mantle cell lymphoma, and others) have been described. This review aims to explore the use of flow cytometry to refine the diagnosis of canine BCL. Particular emphasis will be given to the possible identification of peculiar immunotypes, putative prognostic markers, staging and minimal residual disease.

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Section: Differentiating Reactive and Neoplastic B-cellsmentioning

confidence: 99%

Flow Cytometry in the Diagnosis of Canine B-Cell Lymphoma

Riondato

Comazzi

2021

Front. Vet. Sci.

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“…The liver may appear normal or diffusely enlarged and hyperechoic or hypoechoic though there are reports of discrete, hypoechoic masses as seen in this case. 12,13 The spleen is typically enlarged and hypoechoic with a diffuse heterogeneous texture commonly referred to as "Swiss cheese appearance." Finally, affected lymph nodes appear enlarged, rounded, and hypoechoic.…”

Section: Discussionmentioning

confidence: 99%

Ultrasonographic diagnosis of a lymphomatous lymph node presumptively infiltrating the ureter causing hydronephrosis in a 3‐year‐old dog

Dempsey,

Moon,

Holland

et al. 2023

Vet Radiology Ultrasound

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A 3‐year‐old male neutered mixed breed dog was presented for chronic vomiting and diarrhea. Abdominal ultrasound revealed a large amorphous, heterogeneous mass within the left mid to caudal abdomen most consistent with medial iliac lymph node. It appeared to invade the left ureter and extend distally causing ureteral obstruction and hydronephrosis. Concurrent additional ultrasound findings were consistent with metastatic or multicentric neoplasia. Fine needle aspirates of the lymph node and spleen both confirmed large cell lymphoma. These findings present evidence of lymphoma invading directly from an organ into the ureter which has not previously been reported in dogs.

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“…In both cases, aggregated or scattered large B cells intermixed with variable numbers of small T cells were reported histologically [ 6 , 7 ]. There is also a report of an indolent hepatic lymphoma in an 11-year-old Golden Retriever that was comprised of clonal small B cells surrounding larger numbers of small to intermediate, mixed T cells, forming atypical follicular structures [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Use of immune repertoire sequencing to resolve discordant microscopic and immunochemical findings in a case of T cell-rich large B cell lymphoma in a young dog

et al. 2021

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Background Lymphocytic neoplasms with frequent reactive lymphocytes are uncommonly reported in dogs, and can pose a diagnostic challenge. Different diagnostic modalities such as cytology, flow cytometry, histopathology, immunohistochemistry, and clonality testing, are sometimes required for a diagnosis. This report illustrates the value of using a multi-modal diagnostic approach to decipher a complex lymphocytic tumor, and introduces immune repertoire sequencing as a diagnostic adjunct. Case presentation A 10-month-old Great Dane was referred for marked ascites. Cytologic analysis of abdominal fluid and hepatic aspirates revealed a mixed lymphocyte population including numerous large lymphocytes, yielding a diagnosis of lymphoma. Flow cytometrically, abdominal fluid lymphocytes were highly positive for CD4, CD5, CD18, CD45, and MHC II, consistent with T cell lymphoma. Due to a rapidly deteriorating clinical condition, the dog was euthanized. Post mortem histologic evaluation showed effacement of the liver by aggregates of B cells surrounded by T cells, suggestive of hepatic T cell-rich large B cell lymphoma. Immune repertoire sequencing confirmed the presence of clonal B cells in the liver but not the abdominal fluid, whereas reactive T cells with shared, polyclonal immune repertoires were found in both locations. Conclusions T cell-rich large B cell lymphoma is a rare neoplasm in dogs that may be challenging to diagnose and classify due to mixed lymphocyte populations. In this case, the results of histopathology, immunohistochemistry and immune repertoire sequencing were most consistent with a hepatic B cell neoplasm and reactive T cells exfoliating into the abdominal fluid. Immune repertoire sequencing was helpful in delineating neoplastic from reactive lymphocytes and characterizing repertoire overlap in both compartments. The potential pitfalls of equating atypical cytomorphology and monotypic marker expression in neoplasia are highlighted.

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Indolent T‐cell‐rich small B‐cell hepatic lymphoma in a Golden Retriever

Cited by 3 publications

References 53 publications

Flow Cytometry in the Diagnosis of Canine B-Cell Lymphoma

Flow Cytometry in the Diagnosis of Canine B-Cell Lymphoma

Ultrasonographic diagnosis of a lymphomatous lymph node presumptively infiltrating the ureter causing hydronephrosis in a 3‐year‐old dog

Use of immune repertoire sequencing to resolve discordant microscopic and immunochemical findings in a case of T cell-rich large B cell lymphoma in a young dog

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