Indolent T-cell large granular lymphocyte leukaemia after haematopoietic SCT: a clinicopathologic and molecular analysis

Abstract

Four women and three men after allogeneic (n ¼ 4) and autologous (n ¼ 3) haematopoietic SCT (HSCT) were observed to have an increase in T-cell large granular lymphocytes (T-LGLs) of CD3 þ CD8 þ phenotype for a median of 41 (15 --118) months. Clonal rearrangement of the T-cell receptor gene was verified by two PCR techniques and direct DNA sequencing, confirming that the cases were neoplastic and therefore classifiable as T-LGL leukaemia. In the allogeneic HSCT cases, T-LGL leukaemia was derived from donor T cells in three patients, as shown by DNA chimerism analysis, and recipient T cells in one patient who had graft failure previously. None of the patients showed cytopenia, autoimmune phenomenon or organ infiltration, which were features typical of de novo T-LGL leukaemia. Six patients had remained asymptomatic with stable large granular lymphocyte counts. One patient died from cerebral relapse of the original lymphoma. T-LGL leukaemias occurring post-HSCT are distinct from de novo T-LGL leukaemia and may have a different pathogenesis and clinical course. Patients did not require specific treatment, and the disease remained stable for long periods.Bone Marrow Transplantation (2012) 47, 952 --956;

Type II enteropathy‐associated T‐cell lymphoma: A multicenter analysis from the Asia Lymphoma Study Group

“…This observation is consistent with a recent study of EATL in 18 Chinese patients, which also showed only type II histology [10]. In several previous studies of intestinal T-cell lymphomas in Asian patients [6][7][8][9]11,12,17,18], type II EATL was also predominantly observed, although fewer than 10 cases of apparently classical EATL were described. However, the diagnosis of classical EATL in these few cases had been queried, based on the lack of stringency in diagnosis, doubtful histologic features, and immunophenotypes that would in fact be consistent with type II EATL [10].…”

“…Other additional markers were performed according to the discretion of the reporting histopathologists. Epstein-Barr Virus (EBV)-encoded RNA (EBER) was detected by in situ hybridization (ISH), and T-cell receptor (TCR) gene clonal rearrangement by polymerase chain reaction (PCR) as previously reported [12].…”

Allogeneic haematopoietic stem cell transplantation for erythropoietic protoporphyria: A cautionary note

“…No acute graft-versus-host disease (GVHD) occurred. Despite the apparent successful haematopoietic engraftment three months post-HSCT, DNA chimerism studies of peripheral blood with short tandem repeat analysis [1] showed only 33% donor chimerism. Expectedly, plasma fluorescence emission scanning still showed an abnormal peak.…”

Molecular evidence for repertoire skewing of T large granular lymphocyte proliferation after allogeneic hematopoietic SCT: report of two cases

“…Among patients undergoing allo-HCT, T-LGL lymphoproliferation can be either asymptomatic 1,4 or accompanied by cytopenias and autoimmune manifestations. 2 Here, both patients experienced unexplained neutropenia persisting for several months; immunology and virology were negative, while the rapid onset of neutropenia after the first administration of Rituximab precluded the diagnosis of the recently recognized syndrome of Rituximab-associated late-onset neutropenia.…”