Indole acetic acid overproduction transformants of the rhizobacterium Pseudomonas sp. UW4

Duca, Daiana; Rose, David R.; Glick, Bernard R.

doi:10.1007/s10482-018-1051-7

Cited by 39 publications

(23 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, PGPB that produce IAA under saline conditions may supply an additional amount of this phytohormone to plants. The supplementary IAA may help to stimulate root growth and partially reverse the growth inhibiting effects of salt stress in both shoot and root growth, as well as improving other physiological parameters like chlorophyll content (Duca et al, 2018). In this regard, the mutants constructed here did not show any impairment in IAA synthesis, since all of them showed similar production when compared to the wild-type strain (Table 3).…”

Section: Discussionmentioning

confidence: 99%

The Production of ACC Deaminase and Trehalose by the Plant Growth Promoting Bacterium Pseudomonas sp. UW4 Synergistically Protect Tomato Plants Against Salt Stress

et al. 2019

Self Cite

View full text Add to dashboard Cite

Soil salinity is a major problem in agriculture. However, crop growth and productivity can be improved by the inoculation of plants with beneficial bacteria that promote plant growth under stress conditions such as high salinity. Here, we evaluated 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity and trehalose accumulation of the plant growth promoting bacterium Pseudomonas sp. UW4. Mutant strains (mutated at acdS, treS , or both) and a trehalose over-expressing strain ( OxtreS ) were constructed. The acdS mutant was ACC deaminase minus; the treS - strain significantly decreased its accumulation of trehalose, and the double mutant was affected in both characteristics. The OxtreS strain accumulated more trehalose than the wild-type strain UW4. Inoculating tomato plants subjected to salt stress with these strains significantly impacted root and shoot length, total dry weight, and chlorophyll content. The evaluated parameters in the single acdS and treS mutants were impaired. The double acdS / treS mutant was negatively affected to a greater extent than the single-gene mutants, suggesting a synergistic action of these activities in the protection of plants against salt stress. Finally, the OxtreS overproducing strain protected tomato plants to a greater extent under stress conditions than the wild-type strain. Taken together, these results are consistent with the synergistic action of ACC deaminase and trehalose in Pseudomonas sp. UW4 in the protection of tomato plants against salt stress.

show abstract

Section: Discussionmentioning

confidence: 99%

The Production of ACC Deaminase and Trehalose by the Plant Growth Promoting Bacterium Pseudomonas sp. UW4 Synergistically Protect Tomato Plants Against Salt Stress

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…brasilense on the plant can vary according to the concentration of the inoculant [66,70]. In general, plant hormones are stimulatory only at certain concentrations, which should not exceed the stimulatory threshold specific to each plant genotype [71]. The higher concentration of A .…”

Section: Discussionmentioning

confidence: 99%

Additive and heterozygous (dis)advantage GWAS models reveal candidate genes involved in the genotypic variation of maize hybrids to Azospirillum brasilense

et al. 2019

View full text Add to dashboard Cite

Maize genotypes can show different responsiveness to inoculation with Azospirillum brasilense and an intriguing issue is which genes of the plant are involved in the recognition and growth promotion by these Plant Growth-Promoting Bacteria (PGPB). We conducted Genome-Wide Association Studies (GWAS) using additive and heterozygous (dis)advantage models to find candidate genes for root and shoot traits under nitrogen (N) stress and N stress plus A. brasilense. A total of 52,215 Single Nucleotide Polymorphism (SNP) markers were used for GWAS analyses. For the six root traits with significant inoculation effect, the GWAS analyses revealed 25 significant SNPs for the N stress plus A. brasilense treatment, in which only two were overlapped with the 22 found for N stress only. Most were found by the heterozygous (dis)advantage model and were more related to exclusive gene ontology terms. Interestingly, the candidate genes around the significant SNPs found for the maize–A. brasilense association were involved in different functions previously described for PGPB in plants (e.g. signaling pathways of the plant's defense system and phytohormone biosynthesis). Our findings are a benchmark in the understanding of the genetic variation among maize hybrids for the association with A. brasilense and reveal the potential for further enhancement of maize through this association.

show abstract

“…Interestingly, we found a gene encoding a nitrilase ( nit , EI613_03300), which is 74% similar that encoded by a nit that converts indole-3-acetonitrile (IAN) directly into IAA or into indol-3-acetamide (IAM) in the rhizobacterium Pseudomonas sp. UW4 [53, 54]. In the latter pathway, the conversion of IAM into IAA requires the action of IAM-hydrolases, for which we found two candidate genes, EI613_04545 e EI613_07015.…”

Section: Resultsmentioning

confidence: 99%

Characterization of cellular, biochemical and genomic features of the diazotrophic plant growth-promoting bacteriumAzospirillumsp. UENF-412522, a novel member of theAzospirillumgenus

Rodrigues

Matteoli

Gazara

et al. 2021

Preprint

View full text Add to dashboard Cite

Given their remarkable beneficial effects on plant growth, several Azospirillum isolates currently integrate the formulations of various commercial inoculants. Our research group isolated a new strain, Azospirillum sp. UENF-412522, from passion fruit rhizoplane. This isolate uses carbon sources that are partially distinct from closely-related Azospirillum isolates. Scanning electron microscopy analysis and population counts demonstrate the ability of Azospirillum sp. UENF-412522 to colonize the surface of passion fruit roots. In vitro assays demonstrate the ability of Azospirillum sp. UENF-412522 to fix atmospheric nitrogen, to solubilize phosphate and to produce indole-acetic acid. Passion fruit plantlets inoculated with Azospirillum sp. UENF-41255 showed increased shoot and root fresh matter, as well as root dry matter, further highlighting its biotechnological potential for agriculture. We sequenced the genome of Azospirillum sp. UENF-412522 to investigate the genetic basis of its plant-growth promotion properties. We identified the key nif genes for nitrogen fixation, the complete PQQ operon for phosphate solubilization, the acdS gene that alleviates ethylene effects on plant growth, and the napCAB operon, which produces nitrite under anoxic conditions. We also found several genes conferring resistance to common soil antibiotics, which are critical for Azospirillum sp. UENF-412522 survival in the rhizosphere. Finally, we also assessed the Azospirillum pangenome and highlighted key genes involved in plant growth promotion. A phylogenetic reconstruction of the genus was also conducted. Our results support Azospirillum sp. UENF-412522 as a good candidate for bioinoculant formulations focused on plant growth promotion in sustainable systems.

show abstract

Indole acetic acid overproduction transformants of the rhizobacterium Pseudomonas sp. UW4

Cited by 39 publications

References 67 publications

The Production of ACC Deaminase and Trehalose by the Plant Growth Promoting Bacterium Pseudomonas sp. UW4 Synergistically Protect Tomato Plants Against Salt Stress

The Production of ACC Deaminase and Trehalose by the Plant Growth Promoting Bacterium Pseudomonas sp. UW4 Synergistically Protect Tomato Plants Against Salt Stress

Additive and heterozygous (dis)advantage GWAS models reveal candidate genes involved in the genotypic variation of maize hybrids to Azospirillum brasilense

Characterization of cellular, biochemical and genomic features of the diazotrophic plant growth-promoting bacteriumAzospirillumsp. UENF-412522, a novel member of theAzospirillumgenus

Contact Info

Product

Resources

About