Indole 3-Butyric Acid Metabolism and Transport in Arabidopsis thaliana

Damodaran, Suresh; Strader, Lucia C.

doi:10.3389/fpls.2019.00851

Cited by 63 publications

(44 citation statements)

References 75 publications

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“…Since IBA is the most common compound used for the induction of ARs, it has also been analyzed to understand its role within the process. It is important to recall that, so far, IBA is believed to act through its conversion to IAA (indole-3-acetic acid) [ 49 ], though some results suggest that in specific responses, IBA itself displays auxin activity [ 50 ].…”

Section: Biochemical Profiles During Armentioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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The genus Castanea includes several tree species that are relevant because of their geographical extension and their multipurpose character, that includes nut and timber production. However, commercial exploitation of the trees is hindered by several factors, particularly by their limited regeneration ability. Regardless of recent advances, there exists a serious limitation for the propagation of elite genotypes of chestnut due to decline of rooting ability as the tree ages. In the present review, we summarize the research developed in this genus during the last three decades concerning the formation of adventitious roots (ARs). Focusing on cuttings and in vitro microshoots, we gather the information available on several species, particularly C. sativa, C. dentata and the hybrid C.sativa × C. crenata, and analyze the influence of several factors on the achievements of the applied protocols, including genotype, auxin treatment, light regime and rooting media. We also pay attention to the acclimation phase, as well as compile the information available about biochemical and molecular related aspects. Furthermore, we considerate promising biotechnological approaches that might enable the improvement of the current protocols.

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Section: Biochemical Profiles During Armentioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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“…Exogenous Supplementation with IAA but Not IBA Restored Defective Gravitropic Responses of npf7.3. IBA is proposed to be converted to IAA through a process similar to fatty acid β-oxidation in peroxisomes, at least when applied exogenously to Arabidopsis (30). Therefore, we hypothesized that IBA taken inside the cells mediated by NPF7.3 has to be converted to IAA to induce physiological responses.…”

Section: Resultsmentioning

confidence: 99%

The Arabidopsis NRT1/PTR FAMILY protein NPF7.3/NRT1.5 is an indole-3-butyric acid transporter involved in root gravitropism

Watanabe

Takahashi

Kanno

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Active membrane transport of plant hormones and their related compounds is an essential process that determines the distribution of the compounds within plant tissues and, hence, regulates various physiological events. Here, we report that the Arabidopsis NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY 7.3 (NPF7.3) protein functions as a transporter of indole-3-butyric acid (IBA), a precursor of the major endogenous auxin indole-3-acetic acid (IAA). When expressed in yeast, NPF7.3 mediated cellular IBA uptake. Loss-of-function npf7.3 mutants showed defective root gravitropism with reduced IBA levels and auxin responses. Nevertheless, the phenotype was restored by exogenous application of IAA but not by IBA treatment. NPF7.3 was expressed in pericycle cells and the root tip region including root cap cells of primary roots where the IBA-to-IAA conversion occurs. Our findings indicate that NPF7.3-mediated IBA uptake into specific cells is required for the generation of appropriate auxin gradients within root tissues.

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“…IBA is proposed to be converted to IAA through a process similar to fatty acid ß-oxidation in peroxisomes, at least when applied exogenously to Arabidopsis 27 . Therefore, we hypothesized that IBA taken inside the cells mediated by NPF7.3 has to be converted to IAA to induce physiological responses.…”

Section: Resultsmentioning

confidence: 99%

The Arabidopsis NRT1/PTR FAMILY Protein NPF7.3/NRT1.5 is an Indole-3-butyric Acid Transporter Involved in Root Gravitropism

Watanabe

Takahashi

Kanno

et al. 2020

Preprint

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17Active membrane transport of plant hormones and their related compounds is an essential process 18 that determines the distribution of the compounds within plant tissues and, hence, regulates various 19 physiological events. Here, we report that the Arabidopsis NITRATE TRANSPORTER 1/PEPTIDE 20 TRANSPORTER FAMILY 7.3 (NPF7.3) protein functions as a transporter of indole-3-butyric acid 21 (IBA), a precursor of the major endogenous auxin indole-3-acetic acid (IAA). When expressed in 22Auxin is the best-characterized mobile plant hormone that plays crucial roles in many 41 aspects of plant growth and development by mainly regulating cell division, cell elongation and cell 42 differentiation 1 . Indole-3-acetic acid (IAA) is the major naturally occurring auxin. Measurements of 43 IAA by mass spectrometry as well as visualization of auxin responses by reporter genes have 44 suggested that IAA is differentially distributed within plant tissues. Furthermore, appropriate IAA 45 gradients or local IAA maxima/minima are required for proper auxin functions. In fact, mutants 46 impaired in IAA transport have been isolated based on their defects in organ development and 47 (also called as CHL1 or NRT1.1) is a well-characterized dual-affinity nitrate transporter that 65 mediates nitrogen uptake from the rhizosphere, however, the same protein also transports IAA 17,18 . 66More recently, the Arabidopsis NPF5.12 protein, also named TRANSPORTER OF IBA1 (TOB1), 67 was identified as an IBA transporter that localizes to the vacuolar membrane 14 . Here, we report that 68 NPF7.3, originally identified as a low-affinity nitrate transporter (NRT1.5) and later shown to be a 69 proton/potassium antiporter in Arabidopsis 19,20 , functions as an IBA transporter. We found that 70 npf7.3 mutant roots were not able to respond properly to gravity. Our transport assays in yeast 71 demonstrated that NPF7.3 efficiently mediated IBA uptake into the cells. Asymmetric distribution of 72 auxin activities in root tips following the reorientation of roots was not observed in the npf7.3 73 mutants, and the defect was restored by IAA but not by IBA treatment. These results suggest that 74 cellular IBA uptake mediated by NPF7.3 contributes to the production of IAA required to fully 75 induce Arabidopsis root gravitropism. 76 77 78Results 79 Mutants defective in NPF7.3 exhibit altered root gravitropism 80Some of the 53 members of the Arabidopsis NPF proteins transport plant hormones such as IAA, 81 abscisic acid (ABA), gibberellin (GA) and jasmonates (jasmonoyl isoleucine; JA-Ile) 16 . Thus, we 82 compound is considered to be an IAA precursor 25,26 . Interestingly, IBA also accumulated at a much 126 higher level in the cells expressing NPF7.3 compared to the control cells (Fig. 2b). Moreover, IBA 127 was more efficiently taken into the cells expressing NPF7.3 than IAA. 128Kinetic analyses indicated that the Km values of NPF7.3 for IAA and IBA were 129 comparable (14 µM and 11 µM for IAA and IBA, respectively), however, NPF7.3 had a much higher 130Vmax va...

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Indole 3-Butyric Acid Metabolism and Transport in Arabidopsis thaliana

Cited by 63 publications

References 75 publications

Recent Advances in Adventitious Root Formation in Chestnut

Recent Advances in Adventitious Root Formation in Chestnut

The Arabidopsis NRT1/PTR FAMILY protein NPF7.3/NRT1.5 is an indole-3-butyric acid transporter involved in root gravitropism

The Arabidopsis NRT1/PTR FAMILY Protein NPF7.3/NRT1.5 is an Indole-3-butyric Acid Transporter Involved in Root Gravitropism

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