Indole-3-acetic acid-induced oxidative burst and an increase in cytosolic calcium ion concentration in rice suspension culture

Abstract

Indole-3-acetic acid (IAA) is the major natural auxin involved in the regulation of a variety of growth and developmental processes such as division, elongation, and polarity determination in growing plant cells. It has been shown that dividing and/or elongating plant cells accompanies the generation of reactive oxygen species (ROS) and a number of reports have suggested that hormonal actions can be mediated by ROS through ROS-mediated opening of ion channels. Here, we surveyed the link between the action of IAA, oxidative burst, and calcium channel activation in a transgenic cells of rice expressing aequorin in the cytosol. Application of IAA to the cells induced a rapid and transient generation of superoxide which was followed by a transient increase in cytosolic Ca(2+) concentration ([Ca(2+)]c). The IAA-induced [Ca(2+)]c elevation was inhibited by Ca(2+) channel blockers and a Ca(2+) chelator. Furthermore, ROS scavengers effectively blocked the action of IAA on [Ca(2+)]c elevation.

Indoleacetic Acid Levels in Wheat and Rice Seedlings under Oxygen Deficiency and Subsequent Reoxygenation

“…Over the last decade there has been a wide spectrum of investigations concerning IAA and ROS interaction. Usually, IAA treatment stimulates ROS production and ROS production, in turn, intensifies oxidative IAA breakdown [64][65][66][67][68]. Our data on IAA treatments contradict these findings; but there are also reports showing the opposite effect of IAA treatment.…”

ROS-Activated Ion Channels in Plants: Biophysical Characteristics, Physiological Functions and Molecular Nature

“…AtrbohD and AtrbohF [ 117 , 118 ] may cross-talk with AtCNGC2, 4, 11 and 12 [ 119 ] to form ‘ROS-Ca 2+ hub’ mediating this reaction. The following hormones are potentially involved in the ROS-Ca 2+ hub: abscisic acid (AtrbohD and AtrbohF cross-talking with AtCNGC5 and AtCNGC6) [ 53 ], auxins (AtRbohD/AtCNGC14) [ 120 , 121 , 122 ], methyl jasmonate (AtrbohD and AtrbohF/AtCNGC2) [ 123 , 124 ] and salicylic acid (AtrbohD/AtGLR3.3) [ 125 , 126 , 127 ]. Drought induces stomata closure via abscisic acid-mediated activation of AtrbohD and AtrbohF/AtCNGC5 and AtCNGC6 couples [ 128 ].…”

The involvement of calmodulin and protein kinases in the upstream of cytosolic and nucleic calcium signaling induced by hypoosmotic shock in tobacco cells

“…Some studies suggested that ROS could be a factor triggering the elevation of [Ca 2+ ] in plant cells. 29,[41][42][43] Indeed, one of the earliest events in response to Hypo-OS is the production of reactive oxygen species (ROS) which is mediated by NADPH oxidases. 44,45 Hence, ROS might participate in the upstream of Hypo-OS-induced Ca 2+ elevation and therefore the role for ROS must be elucidated in the future studies.…”