Indole-3-Acetic Acid Biosynthesis Pathways in the Plant-Beneficial Bacterium Arthrobacter pascens ZZ21

Li, Mengsha; Guo, Rui; Yu, Fei; Xu, Chuncheng; Zhao, Haiyan; Li, Huixin; Wu, Jun

doi:10.3390/ijms19020443

Cited by 98 publications

(84 citation statements)

References 58 publications

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“…The results showed that most of the isolates could synthesize IAA, but their production rates were quite different. This variation is due to the different synthetic pathways, key genes and regulatory strategies of different bacteria [30]. Bacteria isolated from the rhizosphere of soybean and rice have been shown to produce 10.54 to 84.72 mg/kg IAA [31,32].…”

Section: Discussionmentioning

confidence: 99%

Beneficial bacteria activate nutrients and promote wheat growth under conditions of reduced fertilizer application

et al. 2020

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Background: Excessive application of chemical fertilizer has exerted a great threat to soil quality and the environment. The inoculation of plants with plant-growth-promoting rhizobacteria (PGPR) has emerged as a great prospect for ecosystem recovery. The aim of this work to isolate PGPRs and highlights the effect of bacterial inoculants on available N/P/K content in soil and on the growth of wheat under conditions of reduced fertilizer application. Results: Thirty-nine PGPRs were isolated and tested for their growth-promoting potential. Thirteen isolates had nitrogen fixation ability, of which N9 (Azotobacter chroococcum) had the highest acetylene reduction activity of 156.26 nmol/gh. Eleven isolates had efficient phosphate solubilizing ability, of which P5 (Klebsiella variicola) released the most available phosphorus in liquid medium (231.68 mg/L). Fifteen isolates had efficient potassium solubilizing ability, of which K13 (Rhizobium larrymoorei) released the most available potassium in liquid medium (224.66 mg/L). In culture medium supplemented with tryptophan, P9 (Klebsiella pneumoniae) produced the greatest amount of IAA. Inoculation with the bacterial combination K14 + 176 + P9 + N8 + P5 increased the alkali-hydrolysed nitrogen, available phosphorus and available potassium in the soil by 49.46, 99.51 and 19.38%, respectively, and enhanced the N, P, and K content of wheat by 97.7, 96.4 and 42.1%, respectively. Moreover, reducing fertilizer application by 25% did not decrease the available nitrogen, phosphorus, and potassium in the soil and N/P/K content, plant height, and dry weight of wheat. Conclusions: The bacterial combination K14 + 176 + P9 + N8 + P5 is superior candidates for biofertilizers that may reduce chemical fertilizer application without influencing the normal growth of wheat.

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Section: Discussionmentioning

confidence: 99%

Beneficial bacteria activate nutrients and promote wheat growth under conditions of reduced fertilizer application

et al. 2020

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“…Although not as common as that in growth‐promoting bacteria, auxin has been shown to be produced by various beneficial fungi to promote plant root growth and biomass (Li et al, ; Sardar & Kempken, ; Zhang, Gan, & Xu, ), for example, the free‐living beneficial fungus T. virens and T. atroviride promote lateral root growth in Arabidopsis , and the growth‐promoting effect is reduced in mutants that are defective in auxin signalling and transport. The auxin‐related compounds IAA, indole‐3‐acetaldehyde, and indole‐3‐ethanol have been detected in the supernatant of Trichoderma virens and Trichoderma atroviride liquid cultures upon the addition of tryptophan (Contreras‐Cornejo et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Although not as common as that in growth-promoting bacteria, auxin has been shown to be produced by various beneficial fungi to promote plant root growth and biomass (Li et al, 2018;Sardar & Kempken, 2018;Zhang, Gan, & Xu, 2019), for example, the free-living Transcript levels of genes identified in (a) were significantly upregulated after Arabidopsis exudate treatment. RNA was extracted from F. oryzae hypha and spores at 7 days after cultivation with or without the addition of Arabidopsis (At) exudate, and the transcript levels were determined by quantitative RT-PCR.…”

Section: Discussionmentioning

confidence: 99%

Endophytic fungus Falciphora oryzae promotes lateral root growth by producing indole derivatives after sensing plant signals

Sun

Wang

et al. 2019

Plant Cell & Environment

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The endophytic fungus Falciphora oryzae was initially isolated from wild rice (Oryza granulata) and colonizes many crop species and promotes plant growth. However, the molecular mechanisms underlying F. oryzae‐mediated growth promotion are still unknown. We found that F. oryzae was able to colonize Arabidopsis thaliana. The most dramatic change after F. oryzae inoculation was observed in the root architecture, as evidenced by increased lateral root growth but reduced primary root length, similar to the effect of auxin, a significant plant growth hormone. The expression of genes responsible for auxin biosynthesis, transport, and signalling was regulated in Arabidopsis roots after F. oryzae cocultivation. Indole derivatives were detected at significantly higher levels in liquid media after cocultivation compared with separate cultivation of Arabidopsis and F. oryzae. Consistently, the expression of indole biosynthetic genes was highly upregulated in F. oryzae upon treatment with Arabidopsis exudates. Global analysis of Arabidopsis gene expression at the early stage after F. oryzae cocultivation suggested that signals were exchanged to initiate Arabidopsis–F. oryzae interactions. All these results suggest that signalling molecules from Arabidopsis roots are perceived by F. oryzae and induce the biosynthesis of indole derivatives in F. oryzae, consequently stimulating Arabidopsis lateral root growth.

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“…Likewise, P. aeruginosa, P. putida and P. fluorescens can modulate root system architecture through an auxin signaling response mediated by cyclodipeptides [4,18]. Bacterial IAA synthesis mainly depends on tryptophan (Trp) via five distinct pathways including indole-3-acetamide (IAM), indole-3-acetonitrile (IAN), indole-3-pyruvic acid (IPyA), tryptamine (TAM) and tryptophan side-chain oxidase (TSO) pathways [19][20][21]. Some bacterial VOCs such as 2,3-butanediol, 3-hydroxy-2butanone, 2-pentylfuran, N,N-dimethyl-hexadecanamine, carbon dioxide (CO 2 ), 13-tetradecadien-1-ol, 2-butanone and 2-methyl-n-1-tridecene promote plant growth and modulate root system architecture.…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas PS01 Isolated from Maize Rhizosphere Alters Root System Architecture and Promotes Plant Growth

2020

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The objectives of this study were to evaluate the plant growth promoting effects on Arabidopsis by Pseudomonas sp. strains associated with rhizosphere of crop plants grown in Mekong Delta, Vietnam. Out of all the screened isolates, Pseudomonas PS01 isolated from maize rhizosphere showed the most prominent plant growth promoting effects on Arabidopsis and maize (Zea mays). We also found that PS01 altered root system architecture (RSA). The full genome of PS01 was resolved using high-throughput sequencing. Phylogenetic analysis identified PS01 as a member of the Pseudomonas putida subclade, which is closely related to Pseudomonas taiwanensis.. PS01 genome size is 5.3 Mb, assembled in 71 scaffolds comprising of 4820 putative coding sequence. PS01 encodes genes for the indole-3-acetic acid (IAA), acetoin and 2,3-butanediol biosynthesis pathways. PS01 promoted the growth of Arabidopsis and altered the root system architecture by inhibiting primary root elongation and promoting lateral root and root hair formation. By employing gene expression analysis, genetic screening and pharmacological approaches, we suggested that the plant-growth promoting effects of PS01 and the alteration of RSA might be independent of bacterial auxin and could be caused by a combination of different diffusible compounds and volatile organic compounds (VOCs). Taken together, our results suggest that PS01 is a potential candidate to be used as bio-fertilizer agent for enhancing plant growth.

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Indole-3-Acetic Acid Biosynthesis Pathways in the Plant-Beneficial Bacterium Arthrobacter pascens ZZ21

Cited by 98 publications

References 58 publications

Beneficial bacteria activate nutrients and promote wheat growth under conditions of reduced fertilizer application

Beneficial bacteria activate nutrients and promote wheat growth under conditions of reduced fertilizer application

Endophytic fungus Falciphora oryzae promotes lateral root growth by producing indole derivatives after sensing plant signals

Pseudomonas PS01 Isolated from Maize Rhizosphere Alters Root System Architecture and Promotes Plant Growth

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