Indole‐3‐acetate beta‐glucosyltransferase <i>OsIAGLU</i> regulates seed vigour through mediating crosstalk between auxin and abscisic acid in rice

He, Yuqing; Zhao, Jia; Yang, Bin; Sun, Shan; Peng, Liling; Wang, Zhoufei

doi:10.1111/pbi.13353

Cited by 53 publications

(52 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effect was also observed in the presence of IBA and ABA ( Figure 3 A), and also under abiotic stress conditions ( Figure 5 A). Our findings were highly consistent with a recent study [ 15 ] reporting that knockout of a rice IAA glycosyltransferase gene IAGLU led to lower seed vigor. The iaglu mutant is sensitive to exogenous application of IBA and ABA, and both IBA and ABA contents were increased in the iaglu mutant, further inducing the expression of ARF genes and ABI genes and leading to lower seed germination and slower postgermination growth [ 15 ].…”

Section: Discussionsupporting

confidence: 93%

“…Seed germination and shoot growth of OE29 and OE30 were observed to be more accelerated than in WT ( Figure 3 A–C), meaning that they are not sensitive to ABA. In a recent study, He et al (2020) examined the interaction between IAA and ABA, and found that exogenous IAA enhanced ABA-mediated reduction of seed vigor in rice [ 15 ]. Accordingly, we also observed that the combination of IBA and ABA suppressed seed germination more strongly than a single hormone ( Figure 3 A–C).…”

Section: Resultsmentioning

confidence: 99%

“…For instance, iaglu was the first IAA glycosyltransferase identified in maize [ 14 ]. The homolog of iaglu was recently identified in rice, and was shown to be involved in catalyzing IAA glycosylation and regulating seed vigor [ 15 ]. In Arabidopsis, a few auxin-related UGTs were also identified, such as UGT84B1 in IAA catalysis, UGT74E2 activity toward indole-3-butyric acid (IBA), and UGT74D1 in IAA and IBA modification [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Overexpression of UGT74E2, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice

Wang

et al. 2020

IJMS

View full text Add to dashboard Cite

UDP-glycosyltransferases (UGTs) play key roles in modulating plant development and responses to environmental challenges. Previous research reported that the Arabidopsis UDP-glucosyltransferase 74E2 (AtUGT74E2), which transfers glucose to indole-3-butyric acid (IBA), is involved in regulating plant architecture and stress responses. Here, we show novel and distinct roles of UGT74E2 in rice. We found that overexpression of AtUGT74E2 in rice could enhance seed germination. This effect was also observed in the presence of IBA and abscisic acid (ABA), as well as salt and drought stresses. Further investigation indicated that the overexpression lines had lower levels of free IBA and ABA compared to wild-type plants. Auxin signaling pathway gene expression such as for OsARF and OsGH3 genes, as well as ABA signaling pathway genes OsABI3 and OsABI5, was substantially downregulated in germinating seeds of UGT74E2 overexpression lines. Consistently, due to reduced IBA and ABA levels, the established seedlings were less tolerant to drought and salt stresses. The regulation of rice seed germination and stress tolerance could be attributed to IBA and ABA level alterations, as well as modulation of the auxin/ABA signaling pathways by UGT74E2. The distinct roles of UGT74E2 in rice implied that complex and different molecular regulation networks exist between Arabidopsis and rice.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of UGT74E2, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice

Wang

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…A recent publication also highlighted a direct role of auxin in seed dormancy (Matilla, 2020). In addition, crosstalk between IAA and ABA in seed development and germination has been reported to occur on both the transcriptional and metabolic level (Chauffour et al, 2019;He et al, 2020).…”

Section: Transcriptomics Analyses Revealed a Link Between Ami1 And Plmentioning

confidence: 99%

Perturbations of theAMI1IAM-amidohydrolase expression trigger plant stress responses inArabidopsis thaliana

Pérez‐Alonso

Ortiz‐García

Moya-Cuevas

et al. 2020

Preprint

View full text Add to dashboard Cite

The evolutionary success of plants relies to a large extent on their extraordinary ability to adapt to changes in their environment. These adaptations require that plants balance their growth with their stress responses. Plant hormones are crucial mediators orchestrating the underlying adaptive processes. However, whether and how the growth-related hormone auxin and the stress-related hormones jasmonic acid (JA), salicylic acid, and abscisic acid (ABA) are coordinated remains largely elusive. Here, we analyze the physiological role of AMIDASE 1 (AMI1) in plant growth and its possible connection to plant adaptations to abiotic stresses. AMI1 contributes to cellular auxin homeostasis by catalyzing the conversion of indole-acetamide into the major plant auxin indole-3-acetic acid. Functional impairment of AMI1 increases the plants’ stress status rendering mutant plants more susceptible to abiotic stresses. Transcriptomic analysis of ami1 mutants disclosed the reprogramming of a considerable number of stress-related genes, including JA and ABA biosynthesis genes. The ami1 mutants exhibit only moderately repressed growth, but an enhanced ABA accumulation, which suggests a role for AMI1 in the crosstalk between auxin and ABA. Altogether, our results suggest that AMI1 is involved in coordinating the trade-off between plant growth and stress responses, balancing auxin with ABA homeostasis.HIGHLIGHTThe IAM amidohydrolase AMI1 catalyzes the conversion of IAM into IAA in vivo. Expression of AMI1 is specifically repressed by osmotic stress conditions, which triggers ABA biosynthesis through the induction of NCED3, thereby linking auxin homeostasis with plant stress responses.

show abstract

“…ABA also regulated lateral root formation and elongation by suppressing the expression of PIN-FORMED1 through ABA-insensitive (ABI4) [33]. In Populus, PdNF-YB21 positively regulates root growth and drought resistance by ABA mediated IAA transport [37].…”

Section: Transcriptome and Regulatory Hormones Of Different Orets Numbermentioning

confidence: 99%

Transcriptome profiling provides new insights into florets number difference of inflorescence in L.angustifolia

Hao

Dong²,

Zhu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Lavender flowers essential oil had been used for a variety of therapeutic and cosmetic purposes, and had been popular for centuries. The previous studies of lavender mainly focused on essential oil composition and extraction methods, ignoring the factors that affected the production of essential oils, such as the floret number. This study aims to get a deep insight into the mechanism of florets number difference. Results 1.Hormone profile showed positive correlation between ABA content and the number of florets, while IAA was negatively correlated. 2. RNA-Seq results showed that 2848 differentially expressed genes (DEG) were screened by comparing different florets samples in one plant. By analyzing dynamic changes of differentially expressed genes, many potentially interesting genes that encoded putative regulators or key components of ABA metabolism and signaling transduction were identified, such as NCED, PYL, PP2C, SnRK2. 3.Common network analysis showed that the key genes of ABA pathway metabolism were negatively related to the DEG of IAA, especial IAA18. 4. Exogenous IAA significantly inhibited the number of lavender florets and affected the expression of ABA pathway metabolism genes, such as NCED, PP2C.Conclusions 1. The different concentrations of ABA lead to florets number difference in the L.angustifolia “JX-2” clusters; 2. ABA may affect the florets number by regulating IAA transport and accumulation.The results will be useful for a better understanding of the molecular mechanism on florets number difference, which will lay the foundation for molecular breeding of muti-flortes varieties.

show abstract

Indole‐3‐acetate beta‐glucosyltransferase OsIAGLU regulates seed vigour through mediating crosstalk between auxin and abscisic acid in rice

Cited by 53 publications

References 36 publications

Overexpression of UGT74E2, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice

Overexpression of UGT74E2, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice

Perturbations of theAMI1IAM-amidohydrolase expression trigger plant stress responses inArabidopsis thaliana

Transcriptome profiling provides new insights into florets number difference of inflorescence in L.angustifolia

Contact Info

Product

Resources

About