Indocyanine Green Uptake and Periodic Acid–Schiff Staining Method for Function Detection of Liver Cells are Affected by Different Cell Confluence

Osteocalcin reduces fat accumulation and inflammatory reaction by inhibiting ROS-JNK signal pathway in chicken embryonic hepatocytes

“…It is easier to get purified hepatocytes using chicken embryonic livers ( Li et al, 2018 ; Wu et al, 2021a ). The purified hepatocytes can be identified by PAS staining which is used to specifically identify them by the markers of carbohydrates and stored glycogens ( Hui et al, 2017 ; Tao et al, 2021 ). In the current study, FE resulted in a high level of TG and a mass of LDs in hepatocytes, which suggests that FE successfully induces hepatocytic steatosis.…”

All-trans retinoic acid inhibits the malignant behaviors of hepatocarcinoma cells by regulating ferroptosis

“…Glycogen storage and metabolism function of mature hepatocyte were measured by PAS staining (Solarbio, Beijing, China) and ICG uptake (Sigma, St. Louis, USA) as previously described. 16 Both of the procedures were performed at least three times in triplicate.…”