Indocyanine Green: Physicochemical Factors Affecting Its Fluorescencein Vivo

Mordon, Serge; Devoisselle, Jean-Marie; Bégu, Sylvie; Desmettre, Thomas

doi:10.1006/mvre.1998.2068

Cited by 233 publications

(168 citation statements)

References 18 publications

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“…This binding of Rh800 to the blood contents like proteins, lipids, etc., which has also been observed with other dyes (2,4,(17)(18)(19)(20), can be exploited to monitor concentrations of blood components that can bind preferentially to selected dyes with high specificity. Kessler and Wolfbeis (4) have shown that AB633 and AB670, two long-wavelength cyanine dyes that bind to HSA preferentially, can be used to monitor the quantity of HSA in urine.…”

Section: Discussionmentioning

confidence: 82%

Fluorescence Properties of Rhodamine 800 in Whole Blood and Plasma

Abugo

Nair

Lakowicz

2000

Analytical Biochemistry

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We have characterized the fluorescence spectral properties of rhodamine 800 (Rh800) in plasma and blood in order to test the possibility of making clinical fluorescence measurements in whole blood without separation steps. Rh800 was used because of its absorption at red/near-infrared wavelengths away from the absorption bands of hemoglobin. We utilized the front-face illumination and detection to minimize the effects of absorption and/or scatter during measurements. The presence of Rh800 was detected in plasma and blood using steady-state fluorescence measurements. Absorption due to hemoglobin reduced the Rh800 intensity from the blood. Fluorescence lifetime measurements in plasma and blood showed that it is possible to recover lifetime parameters of Rh800 in these media. We obtained mean lifetimes of 1.90 and 1.86 ns for Rh800 in plasma and blood, respectively. Using the recently described modulation sensing method, we quantified the concentrations of Rh800 in plasma and blood. Rh800 was detected at a concentration of as low as 2 microM in both media. High anisotropy values were obtained for Rh800 in plasma and blood using steady-state and anisotropy decay measurements, implying the tight binding of this probe to the contents of these media. This binding can be exploited to monitor the concentrations of different blood components using already existing or new red-emitting probes that will be specially designed to bind to these components with high specificity. To test this possibility of direct measurements in blood, we used Rh800 to monitor albumin in the presence of red blood cells. Increase in the polarization of Rh800 as the concentration of albumin was increased in the presence of the red cells showed the feasibility of such measurements.

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Section: Discussionmentioning

confidence: 82%

Fluorescence Properties of Rhodamine 800 in Whole Blood and Plasma

Abugo

Nair

Lakowicz

2000

Analytical Biochemistry

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“…Therefore, we can expect that the interaction between ICG and BSA would be similar to those between HSA and ICG. It is reported that 1-2 ICG molecules bind mainly to the hydrophobic core of HSA [31], [32], [34], [51]. Therefore, when ICG molecules were introduced into the McCoy's 5A cell culture medium containing FBS, they bound to BSA as monomers at their assigned binding sites.…”

Section: Resultsmentioning

confidence: 99%

Revisiting Indocyanine Green: Effects of Serum and Physiological Temperature on Absorption and Fluorescence Characteristics

Jung

Vullev

Anvari

2014

IEEE J. Select. Topics Quantum Electron.

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Abstract-Indocyanine green (ICG) remains as the only near infrared dye approved by the FDA. Despite its long history of usage in clinical medicine, a systematic study of the effects of serum proteins at physiologically relevant levels and temperature on absorption and fluorescence characteristics of ICG has been missing. We incubated ICG at concentrations in the range of 0.6-25.8 μM in McCoy's 5a cell culture medium, without and with supplemental fetal bovine serum (FBS) at 5% and 10% levels. Our analyses of absorption and fluorescence spectra indicate that the peak absorbance of ICG associated with its monomeric form increases in the presence of FBS. For example, at ICG concentration of 25.8 μM, the monomer absorbance is increased by nearly 100% in the presence of 10% FBS. Similarly, there is an increase in the relative fluorescence quantum yield of ICG, by as much as nearly 3.5 times in the presence of FBS. When incubated at 37• C, the presence of FBS in the cell culture medium helps maintain the monomeric absorption of ICG and sustain the increased fluorescence emission. We offer explanations to describe the possible photophysical mechanisms underlying the observed effects and discuss the importance of these results to in-vivo applications of ICG.

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“…Two fluorescence decay functions from selected spots with the corresponding decay parameters are shown in Figure 4B and C. It is known that ICG has a lifetime of about 200 ps in water, and about 600 ps when bound to serum albumin [32][33][34]. In the short-lifetime regions (yellow-orange in Figure 4A) the lifetimes of the decay components are 231 ps and 576 ps ( Figure 4C).…”

Section: Confocal System One-photon Excitation With a Picosecond Diomentioning

confidence: 99%

Fluorescence lifetime imaging with near-infrared dyes

Becker

Shcheslavskiy

2015

Photonics &Amp; Lasers in Medicine

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Near-infrared (NIR) dyes are used as fluorescence markers in small animal imaging and in diffuse optical tomography. In these applications it is important to know whether the dyes bind to proteins or to other tissue constituents, and whether their fluorescence lifetimes depend on the targets they bind to. Unfortunately, neither the optical beam paths nor the detectors of commonly used in confocal and multiphoton laser scanning microscopes (LSMs) directly allow for excitation and detection of NIR fluorescence. This paper presents three ways of adapting existing LSMs with time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) systems for NIR FLIM: 1) confocal systems with wideband beamsplitters and diode laser excitation, 2) confocal systems with wideband beamsplitters and onephoton excitation by titanium-sapphire lasers, and 3) twophoton systems with optical parametric oscillator (OPO) excitation and non-descanned detection. A number of NIR dyes are tested in biological tissue. All of them show clear lifetime changes depending on the tissue structures they are bound to. We therefore believe that NIR FLIM can deliver supplementary information about the tissue composition and on local biochemical parameters.

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Indocyanine Green: Physicochemical Factors Affecting Its Fluorescencein Vivo

Cited by 233 publications

References 18 publications

Fluorescence Properties of Rhodamine 800 in Whole Blood and Plasma

Fluorescence Properties of Rhodamine 800 in Whole Blood and Plasma

Revisiting Indocyanine Green: Effects of Serum and Physiological Temperature on Absorption and Fluorescence Characteristics

Fluorescence lifetime imaging with near-infrared dyes

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