Indocyanine green enhanced near‐infrared laser treatment of murine mammary carcinoma

Abstract

It is well accepted that near-infrared (NIR) lasers are appropriate to ablate benign lesions and induce irreversible thermal injury in deeply seated blood vessels. At this wavelength, the laser light penetrates deep (3–5 mm) into the skin. However, many researchers have reported noticeable pain, extending from mild to severe, during and immediately following NIR laser treatment. Intravenous administration of an exogenous chromophore (indocyanine green, ICG, dye) can effectively convert NIR laser light into heat. In this approach the presence of ICG has shown to enhance thermal injury of blood vessels in the treatment of healthy tissues. However, the effectiveness of thermal injury on the regression of cutaneous carcinomas during ICG/NIR laser therapy has not been assessed. The purpose of this study was to evaluate the potential benefit of using ICG/NIR laser therapy to regress superficial carcinoma with thermal injury. Two groups of A/J mice with subcutaneous mammary adenocarcinoma tumors (7–9 mm) were irradiated with a 808-nm NIR laser preceded by tail vein injection of ICG dye or sterile saline. Histological evaluation of the subcutaneous tissue revealed minor thermal damage and necrosis in the laser/saline group and substantial damage (up to 100% necrosis) in the laser/ICG group. The laser/ICG-treated group showed a steady reduction in tumor volume compared to the laser/saline group: 48% by Day 5 (P=0.045) and 69%–70% by Days 8, 9, and 10 (P values 0.0005 or less). The vascular targeted ICG–NIR laser therapy appears to have potential for treating superficial tumors.

Near-infrared fluorescent probes in cancer imaging and therapy: an emerging field

“…82 It has been demonstrated that ICG promotes the absorption of NIR laser light delivered by a diode laser, inducing more thermal damage to solid tumors after laser irradiation compared to laser alone. 173,174 In addition, local hyperthermia …”

Thermosensitive Liposome Formulated Indocyanine Green for Near-Infrared Triggered Photodynamic Therapy: In Vivo Evaluation for Triple-Negative Breast Cancer

“…Nevertheless, given the potential photothermal capabilities of ICG and preferential accumulation in the tumor, additional localized destruction of tumor cells may be achieved to further enhance the antitumor efficacy of PDT (40,41). Literature has shown that tumorbearing mice administered with ICG and stimulated by NIR laser light at 808 nm exhibited significant tissue necrosis upon histological evaluation, which gradually led to reduction of tumor volume when compared to control groups (42). Hence, LPICG delivery system irradiated by NIR laser light may even be able to achieve selective and targeted dual cytotoxic effects at the tumor site elicited via both PDT and photothermal therapy.…”

A new strategy for utilization of NIR from solar energy—Promotion effect generated from photothermal effect of Fe3O4@SiO2 for photocatalytic oxidation of NO

“…With the irradiation of NIR, the tumor cells containing proper photothermal agents were killed due to high temperature resulted from photothermal effect. Photothermal agents such as organic compounds (indocyanine green [11], polyaniline nanoparticles [12], etc. ), noble metal nanomaterials (Au [13], Ag [14] and Pd [15], etc.…”