Indocyanine green delivery systems for tumour detection and treatments

Porcu, Elena Piera; Salis, Andrea; Gavini, Elisabetta; Rassu, Giovanna; Maestri, Marcello; Giunchedi, Paolo

doi:10.1016/j.biotechadv.2016.04.001

Cited by 155 publications

(110 citation statements)

References 220 publications

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“…Among the latter, indocyanine green (ICG), a clinically approved NIR dye, is one of the most exploited exogenous absorbers so far. 4,[9][10][11] It has an absorption maximum of ~780-800 nm (depending on the environment) and fluoresces in the NIR window. In blood, free ICG binds to plasma proteins and is rapidly excreted.…”

mentioning

confidence: 99%

Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers

Beztsinna

Tsvetkova

Jose

et al. 2017

IJN

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Photoacoustic imaging is an emerging method in the molecular imaging field, providing high spatiotemporal resolution and sufficient imaging depths for many clinical applications. Therefore, the aim of this study was to use photoacoustic imaging as a tool to evaluate a riboflavin (RF)-based targeted nanoplatform. RF is internalized by the cells through a specific pathway, and its derivatives were recently shown as promising tumor-targeting vectors for the drug delivery systems. Here, the RF amphiphile synthesized from a PEGylated phospholipid was successfully inserted into a long-circulating liposome formulation labeled with the clinically approved photoacoustic contrast agent – indocyanine green (ICG). The obtained liposomes had a diameter of 124 nm (polydispersity index =0.17) and had a negative zeta potential of −26 mV. Studies in biological phantoms indicated a stable and concentration-dependent photoacoustic signal (Vevo ® LAZR) of the ICG-containing RF-functionalized liposomes. In A431 cells, a high uptake of RF-functionalized liposomes was found and could be blocked competitively. First, studies in mice revealed ~3 times higher photoacoustic signal in subcutaneous A431 tumor xenografts ( P <0.05) after injection of RF-functionalized liposomes compared to control particles. In this context, the application of a spectral unmixing protocol confirmed the initial quantitative data and improved the localization of liposomes in the tumor. In conclusion, the synthesized RF amphiphile leads to efficient liposomal tumor targeting and can be favorably detected by photoacoustic imaging with a perspective of theranostic applications.

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mentioning

confidence: 99%

Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers

Beztsinna

Tsvetkova

Jose

et al. 2017

IJN

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“…ICG is a near-infrared fluorescent dye that is stimulated by light wavelengths of 750–810 nm that produce near-infrared light with a wavelength of 850 nm (Porcu et al 2016). Near-infrared light can be observed using an ICG–FI system with a PDE and is displayed with the help of a developing device (van der Vorst et al 2012; Porcu et al 2016; Abo et al 2015). …”

Section: Discussionmentioning

confidence: 99%

Liver tumor boundaries identified intraoperatively using real-time indocyanine green fluorescence imaging

Zhang

Shi

Hou

et al. 2016

J Cancer Res Clin Oncol

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PurposeClear delineation between tumors and normal tissues is ideal for real-time surgical navigation imaging. We investigated applying indocyanine green (ICG) fluorescence imaging navigation using an intraoperative administration method in liver resection.MethodsFifty patients who underwent liver resection were divided into two groups based on clinical situation and operative purpose. In group I, sizes of superficial liver tumors were determined; tiny tumors were identified. In group II, the liver resection margin was determined; real-time navigation was performed. ICG was injected intravenously at the beginning of the operation; the liver surface was observed with a photodynamic eye (PDE).ResultsLiver resection margins were determined using PDE. Fluorescence contrast between normal liver and tumor tissues was obvious in 32 of 35 patients. A boundary for half the liver or specific liver segments was determined in nine patients by examining the portal vein anatomy after ICG injection. Eight small tumors not observed preoperatively were detected; the smallest was 2 mm.ConclusionsICG fluorescence imaging navigation is a promising, simple, and safe tool for routine real-time intraoperative imaging during hepatic resection and clinical exploration in hepatocellular carcinoma, enabling high sensibility for identifying liver resection margins and detecting tiny superficial tumors.

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“…This compound has achieved remarkable attention in many clinical applications due to its favourable properties [26]. During the last years, ICG has been employed for the identification of HCC during surgical procedures [27][28][29] proving that ICG is a promising tool for intraoperative imaging during hepatic resection.…”

Section: Introductionmentioning

confidence: 99%

“…The fluorescence of ICG can be detected by using different instruments, including Photodynamic Eye (PDE), which is widely employed in clinical applications [30][31][32][33]. After bonding with plasma proteins in the blood, ICG is excited with near infrared light at 760 nm and emits fluorescence at a slightly longer near infrared wavelength below 820 nm [26].…”

Section: Introductionmentioning

confidence: 99%

Engineered polymeric microspheres obtained by multi-step method as potential systems for transarterial embolization and intraoperative imaging of HCC: Preliminary evaluation

Porcu

Salis

Rassu

et al. 2017

European Journal of Pharmaceutics and Biopharmaceutics

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Indocyanine green delivery systems for tumour detection and treatments

Cited by 155 publications

References 220 publications

Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers

Photoacoustic imaging of tumor targeting with riboflavin-functionalized theranostic nanocarriers

Liver tumor boundaries identified intraoperatively using real-time indocyanine green fluorescence imaging

Engineered polymeric microspheres obtained by multi-step method as potential systems for transarterial embolization and intraoperative imaging of HCC: Preliminary evaluation

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