Indocyanine Green-Conjugated Superparamagnetic Iron Oxide Nanoworm for Multimodality Breast Cancer Imaging

Abstract

Breast cancer is the leading cause of cancer-associated deaths among women. Techniques for non-invasive breast cancer detection and imaging are urgently needed. Multimodality breast cancer imaging is attractive since it can integrate advantages from several modalities, enabling more accurate cancer detection. In order to accomplish this, indocyanine green (ICG)-conjugated superparamagnetic iron oxide nanoworm (NW− ICG) has been synthesized as a contrast agent. When evaluated in a spontaneous mouse breast cancer model, NW−ICG gave a large tumor to normal tissue contrasts in multiple imaging modalities including magnetic particle imaging, near-infrared fluorescence imaging, and photoacoustic imaging, providing more comprehensive detection and imaging of breast cancer. Thus, NW−ICGs are an attractive platform for non-invasive breast cancer diagnosis.

Active Targeting Hyaluronan Conjugated Nanoprobe for Magnetic Particle Imaging and Near-Infrared Fluorescence Imaging of Breast Cancer and Lung Metastasis

“…Diagnosis of metastasis in addition to primary tumor is crucial to patients with breast cancer since a major cause of breast cancer related death is due to metastasis. − While the EPR effects have been often utilized for NP aided cancer diagnosis studies, ,, it is less applicable to breast cancer lung metastasis detection due to the small tumor mass in earlier stages of metastasis as compared to the primary tumor. − Active targeting can be an attractive approach for the diagnosis of breast cancer lung metastasis.…”

“…Nanoparticle-based contrast agents can significantly aid in cancer imaging. Nanoworms (NWs) bearing indocyanine green (ICG) (NW-ICGs) have been applied to primary breast cancer detection in mice. , The accumulation of the nanoprobes in cancer was presumably due to the passive targeting of NWs through the enhanced permeability retention (EPR) effect. − However, the breast cancer lung metastasis typically has small tumor masses compared to the primary tumor; thus, a less prominent EPR effect is expected. , To enhance NP accumulation in metastatic sites, cell surface receptors on tumor cells may be targeted. CD44 is a glycoprotein that is overexpressed on various types of cancerous cells, including breast cancer. , CD44 is known to play a key role in metastasis and relapse of breast cancer. , Hyaluronan (HA) is the major endogenous ligand of CD44. ,− Herein, we report an ICG and HA conjugated iron oxide nanoparticle (NP-ICG-HA), which can bind well with CD44.…”

Recurrent and convolutional neural networks for sequential multispectral optoacoustic tomography (MSOT) imaging

“…The ultrasound transducer is then used to detect this acoustic wave, and the back‐projection algorithm [42] is applied to the detected optoacoustic wave to reconstruct the images. For the dataset preparation, transgenic mice [43] with breast tumors were intravenously injected with ICG‐conjugated superparamagnetic iron oxide nanoworms (NWs‐ICG) [44], which accumulate in tumors longer than pure ICG through the enhanced permeability and retention effect [45]. Twenty‐four hours after injection, the mice were euthanized and the tumors were removed and dissected for this study.…”

WSA-MP-Net: Weak-signal-attention and multi-scale perception network for microvascular extraction in optical-resolution photoacoustic microcopy

“…Photoacoustic imaging (PAI) can non-invasively detect the internal information of the biological tissues from cells to organs based on the photoacoustic effect, which is of great significance for non-destructive imaging research in life science [1] , [2] , [3] . Due to the high blood absorption in the near-infrared region, PAI exhibits unique superiority in imaging vascular structures without exogenous contrast agents.…”