Indocyanine green binds to DOTAP liposomes for enhanced optical properties and tumor photoablation

Miranda, Dyego; Wan, Chao; Kilian, Hailey I.; Mabrouk, Moustafa T.; Zhou, Yuhan; Jin, Honglin; Lovell, Jonathan F.

doi:10.1039/c9bm00551j

Cited by 34 publications

(27 citation statements)

References 45 publications

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“…51 Furthermore, ICG was shown to have enhanced photostability via encapsulation in lipids or association with plasmonic gold colloids. [54][55][56] All these studies clearly point out the versatility of ICG as a theranostic agent and the need for thorough photostability characterization in the presence of other dyes or oxidizing agents in these formulations both in solutions and in cellular environment.…”

Section: Photoacoustic Monitoring Of Indocyanine Green Photodegradatimentioning

confidence: 99%

Mutual impact of clinically translatable near-infrared dyes on photoacoustic image contrast and in vitro photodynamic therapy efficacy

et al. 2020

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Photodynamic therapy (PDT), a spatially localized phototoxic therapy that involves irradiation of a photosensitizer (PS) with specific wavelengths of light, has shown exceptional promise in impacting cancer treatment outcomes, particularly oral cancer. To reduce PDT outcome variability, attempts toward image-guided personalized PDT are being pursued by monitoring PS uptake either via fluorescence or photoacoustic imaging (PAI), a nonionizing modality dependent on optical absorption properties of the tissue. PAI-guided PDT requires a near-infrared contrast agent for deep tissue imaging with minimal photobleaching effect. We evaluate the impact of PDT agent, benzoporphyrin derivative (BPD), on PAI agent indocyanine green (ICG) and vice versa, given that they have different optical absorption properties and singlet oxygen quantum yields for PDT. Specifically, we demonstrate in two oral squamous cell carcinoma lines (FaDu and SCC4) that ICG has minimal effect on BPD PDT efficacy when irradiated with either a continuous or pulsed laser. Furthermore, the impact of BPD on ICG photodegradation was monitored with PAI in tissue-mimicking phantoms. These studies inform us that the combination of BPD and ICG can be utilized for PAI-guided PDT. However, researchers need to consider the photodegradation effects of ICG in the presence of BPD when designing their drug delivery strategies for PAI-guided PDT. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Photoacoustic Monitoring Of Indocyanine Green Photodegradatimentioning

confidence: 99%

Mutual impact of clinically translatable near-infrared dyes on photoacoustic image contrast and in vitro photodynamic therapy efficacy

et al. 2020

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“…1). However, there is robust evidence indicating that ICG strongly binds to proteins and phospholipids (PL), which deeply affect the uorescence yield and half-life [26,27] of the tracer. For this reason, in clinical practice, different ICG-based formulas in which the dye is combined with proteins have been used to improve the retention in target organs [28,29].…”

Section: Discussionmentioning

confidence: 99%

Multimodality Approach to Characterizing the Distinctive Hallmarks of Lung Fibrosis: A Mouse Model with Bleomycin and Indocyanine Green

Grandi

Ferrini

Ciccimarra

et al. 2021

Preprint

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Background.Idiopathic Pulmonary Fibrosis is a progressive disease with short life expectancy and no disease-modifying pharmacological therapy. The continuous refinement of animal models and the integration of in-vivo imaging techniques is fundamental for the selection of new antifibrotic drugs.Indocyanine Green (ICG), a fluorescent dye, was administered by oropharyngeal aspiration (OA) to mice with Bleomycin (BLM) to map the lung exposure.Methods.Female mice C57bl/6 were treated via OA with BLM+ICG or ICG. Animals were imaged at 7, 14 and 21 days either with the fluorescent system or Micro-CT. At each time point subsets of mice were sampled for ex-vivo assessment. Histological assessment of fibrosis by Ashcroft score, airspace enlargements and mean linear intercept (MLI) were evaluated at 7, 14 and 21 days. Leukocytes and cytokines were measured in bronchoalveolar lavage fluid. Results.Fluorescence imaging revealed a persistent lung signal in both groups until 21 days. In BLM+ICG group, Micro-CT detected a marked increase in hypo- and non-aerated tissues throughout the study. At later time points hyper-inflated tissue was detected. Histology revealed high Ashcroft score throughout the time-course with a prominent increase in airspace size and MLI at day 21. ICG mice had healthy lungs.Conclusions.We showed that ICG can be used as a tracer to map the distribution of BLM in lungs. However, BLM+ICG produced unexpected severe lung changes different from pure BLM model, such as emphysema-like features which progressively worsened. The multimodalities approach warranted characterization of the distinctive features of this new pulmonary fibrosis model and provided fundamentals for in-vivo translation.

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“…Even CaP based nanomaterials have some drawbacks like complicated synthetic steps, large size, and obvious aggregation. Its enhanced PTT with chemotherapy can improve anti-cancer efficiency 125 . CaP is a highly biocompatible inorganic biomaterial; readily form with adenovirus, so enhancing adenovirusmediated gene transfer.…”

Section: Calcium Phosphate Nanocarriersmentioning

confidence: 99%

Emerging indocyanine green-integrated nanocarriers for multimodal cancer therapy: a review

2021

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Indocyanine green binds to DOTAP liposomes for enhanced optical properties and tumor photoablation

Abstract: Simple mixing of ICG with DOTAP liposomes results in full dye binding to the liposomes and enhanced ICG optical properties.

Cited by 34 publications

References 45 publications

Mutual impact of clinically translatable near-infrared dyes on photoacoustic image contrast and in vitro photodynamic therapy efficacy

Mutual impact of clinically translatable near-infrared dyes on photoacoustic image contrast and in vitro photodynamic therapy efficacy

Multimodality Approach to Characterizing the Distinctive Hallmarks of Lung Fibrosis: A Mouse Model with Bleomycin and Indocyanine Green

Emerging indocyanine green-integrated nanocarriers for multimodal cancer therapy: a review

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