Individual variability in response to a single sickling event for normal, sickle cell, and sickle trait erythrocytes

Tarasev, Michael; Muchnik, Marina; Light, Lucia; Alfano, K.; Chakraborty, Sumita

doi:10.1016/j.trsl.2016.09.005

Cited by 6 publications

(4 citation statements)

References 53 publications

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“…HbS polymerization is the primary event in SCD. Repeated polymerization cycles cause irreversible damage to red blood cell (RBC) deformability, while a single polymerization results in reversible decreased RBC deformability and increased mechanical fragility [9].…”

Section: Introductionmentioning

confidence: 99%

Sickle Cell Disease: Role of Oxidative Stress and Antioxidant Therapy

et al. 2021

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Sickle cell disease (SCD) is the most common hereditary disorder of hemoglobin (Hb), which affects approximately a million people worldwide. It is characterized by a single nucleotide substitution in the β-globin gene, leading to the production of abnormal sickle hemoglobin (HbS) with multi-system consequences. HbS polymerization is the primary event in SCD. Repeated polymerization and depolymerization of Hb causes oxidative stress that plays a key role in the pathophysiology of hemolysis, vessel occlusion and the following organ damage in sickle cell patients. For this reason, reactive oxidizing species and the (end)-products of their oxidative reactions have been proposed as markers of both tissue pro-oxidant status and disease severity. Although more studies are needed to clarify their role, antioxidant agents have been shown to be effective in reducing pathological consequences of the disease by preventing oxidative damage in SCD, i.e., by decreasing the oxidant formation or repairing the induced damage. An improved understanding of oxidative stress will lead to targeted antioxidant therapies that should prevent or delay the development of organ complications in this patient population.

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Section: Introductionmentioning

confidence: 99%

Sickle Cell Disease: Role of Oxidative Stress and Antioxidant Therapy

et al. 2021

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“…A neat concept to test red blood cell stability based on a bead mill and spectral measurement of hemoglobins was developed by Blaze Medical Devices (Ann Arbor, MI, United States). Although the concept was convincing (Tarasev et al, 2016), the device never entered the market. However, measurements as a service are offered by Functional Fluidics (Detroit, MI, United States).…”

Section: A New Generation Of Devices Probing For Physico-chemical Promentioning

confidence: 99%

Trends in the Development of Diagnostic Tools for Red Blood Cell-Related Diseases and Anemias

Kaestner

Bianchi

2020

Front. Physiol.

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“…In experiments on an isolated rabbit lung, it was shown that the plasticity of the erythrocytes may affect the blood pressure value in it and the pulmonary oxygen diffusion coefficient [31]. The deformability of the erythrocytes due to an increased convection of their contents is more conducive to oxygen transport than facilitated diffusion [32].…”

Section: Group III Resistant Ah N=24mentioning

confidence: 99%

Raman Spectroscopy of Hemoglobin and Dielectrophoresis of Erythrocytes in the Diagnosis of the Resistant Arterial Hypertension

Кручинина

Gromov

Кручинин

et al. 2020

J-BPE

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The research aim is to study the associations of changes in the content of hemoglobin complexes by the Raman spectroscopy with electrical and viscoelastic parameters of erythrocytes (using the dielectrophoresis method) among patients with arterial hypertension (AH) (including resistant AH), to evaluate the possibility of using these parameters for diagnostic purposes. Fifty males (54 ± 6 years) with stage 2 of AH have been examined, 24 of them have showed resistant AH. We have determined a significant decrease in the level of hemoglobin-ligand complexes, Hb-NO (II) complexes in patients with resistant AH compared to those among patients with controlled AH and among healthy patients (p < 0.001-0.05). We have found correlations between intensities of the most important signals of hemoglobin Raman spectra (1325, 1350, 1550, 1580, 1660, 1668 cm-1) and electric and viscoelastic parameters of erythrocytes (amplitude of erythrocytes deformation, summarized indicators of viscosity and rigidity, the magnitude of the dipole moment, polarizability at the frequency 106 Hz, electrical conductivity, index of destruction), the intensity of which had been the biggest one for resistant AH. It has been shown that the combined use of two methods (Raman spectroscopy of hemoglobin and electrical and the studies of erythrocytes by the dielectrophoresis method) allowed to increase the diagnostic accuracy to detect the resistant arterial hypertension up to 88%, sensitivity up to 84.6%, specificity up to 91.7% compared to the data of the combined clinical and instrumental methods of research. The capability of this combination approach exceeds the capabilities of the methods separately.

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Individual variability in response to a single sickling event for normal, sickle cell, and sickle trait erythrocytes

Cited by 6 publications

References 53 publications

Sickle Cell Disease: Role of Oxidative Stress and Antioxidant Therapy

Sickle Cell Disease: Role of Oxidative Stress and Antioxidant Therapy

Trends in the Development of Diagnostic Tools for Red Blood Cell-Related Diseases and Anemias

Raman Spectroscopy of Hemoglobin and Dielectrophoresis of Erythrocytes in the Diagnosis of the Resistant Arterial Hypertension

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