Individual serum levels of anti-Mullerian hormone in healthy girls persist through childhood and adolescence: a longitudinal cohort study

Hagen, Casper P; Aksglæde, Lise; Sørensen, Kaspar; Mouritsen, Annette; Andersson, Anna‐Maria; Petersen, Jørgen Holm; Main, Katharina M.; Juul, Anders

doi:10.1093/humrep/der435

Cited by 120 publications

(103 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We have recently shown that AMH levels in childhood and adolescence are highly stable and appear to accurately reflect the individual follicle pool (Hagen et al 2012) as well as ovarian reserve (Hagen et al 2010). While our present finding that antenatal exposure to phthalates is associated with a suppression of circulating serum FSH in adolescence would appear contradictory, we cannot exclude a potential pituitary programming effect due to a resetting of the hypothalamic-pituitary-ovarian axis following phthalate exposure.…”

Section: Discussioncontrasting

confidence: 76%

The influence of antenatal exposure to phthalates on subsequent female reproductive development in adolescence: a pilot study

Hart¹,

Doherty²,

Frederiksen³

et al. 2014

Reproduction

View full text Add to dashboard Cite

We hypothesised that antenatal exposure to ubiquitous phthalates may lead to an earlier menarche and a lower prevalence of polycystic ovarian syndrome (PCOS) and polycystic ovarian morphology (PCO) in adolescence. The Western Australian Pregnancy Cohort (Raine) Study recruited 3000 women at 18 weeks of gestation in 1989-1991, 1377 had antenatal serum stored without thawing at K80 8C. An unselected subset was evaluated in the early follicular phase for PCO and PCOS by ultrasound and serum evaluation in adolescence. Serum was analysed for anti-Mü llerian hormone (AMH), inhibin B, sex hormone binding globulin (SHBG), testosterone, androstenedione and DHEAS. Four hundred microlitres of the frozen maternal serum underwent isotope-diluted liquid chromatography-tandem mass spectrometry, with preceding enzymatic deconjugation followed by solid-phase extraction to determine phthalate exposure. Two hundred and forty four girls attended assessment and most common phthalate metabolites were detectable in the majority of the 123 samples available. Several phthalates were negatively associated with maternal SHBG, and associations with maternal androgens were less consistent. The sum of the metabolites of di-(2-ethylhexyl) phthalate was associated with a non-significant tendency towards an earlier age at menarche (PZ0.069). Uterine volume was positively associated with mono-(carboxy-iso-octyl) phthalate (PZ0.018). Exposure to monoethyl phthalate (MEP) and the sum of all phthalate metabolites (Sall phth.m) were protective against PCOS in adolescence (PZ0.001 and PZ0.005 respectively). There were negative associations of MEP with PCO (PZ0.022) and of MEP with serum AMH (PZ0.031). Consequently, our data suggest that antenatal exposure to environmental phthalates may be associated with oestrogenic and/or anti-androgenic reproductive effects in adolescent girls.

show abstract

Section: Discussioncontrasting

confidence: 76%

The influence of antenatal exposure to phthalates on subsequent female reproductive development in adolescence: a pilot study

Hart¹,

Doherty²,

Frederiksen³

et al. 2014

Reproduction

View full text Add to dashboard Cite

show abstract

“…Serum AMH concentrations decrease over time to become undetectable at menopause, correlating with the decline in AFC with increasing age (de Vet et al, 2002;van Rooij et al, 2002van Rooij et al, , 2004van Rooij et al, , 2005. In contrast, serum AMH levels increase slightly during mini-puberty and remain constant during adolescence, regulated through a yet unexplained mechanism (Hagen et al, 2012;Lie Fong et al, 2012). Also in our cohort of TS subjects with measurable AMH, we observed a borderline quadratic relationship between AMH and age with a rise during early childhood and a starting decrease in the older age group.…”

Section: Discussionmentioning

confidence: 99%

Anti-Mullerian hormone levels in girls and adolescents with Turner syndrome are related to karyotype, pubertal development and growth hormone treatment

et al. 2013

View full text Add to dashboard Cite

what is known already: Most girls with TS suffer from incomplete sexual development, premature ovarian failure and infertility due to abnormal ovarian folliculogenesis. Serum AMH levels reflect the ovarian reserve in females, even in childhood. study design, size, duration: Cross-sectional study investigating 270 karyotype proven TS patients aged 0-20 years between 2009 and 2010. participants/materials, settings, methods: Studies were conducted at three University Children's hospitals in Europe. Main outcome measures were clinical data concerning pubertal development as well as laboratory data including karyotype, serum AMH, LH, FSH, estradiol (E2), inhibin B and IGF.results and the role of chance: Serum AMH was detectable in 21.9% of all TS girls and correlated strongly with karyotypes.A measurable serum AMH was found in 77% of TS girls with karyotype 45,X/46,XX, in 25% with 'other' karyotypes and in only 10% of 45,X TS girls. A strong relationship was also observed for measurable serum AMH and signs of spontaneous puberty such as breast development [adjusted odds ratio (OR) 19.3; 95% CI 2.1-175.6; P ¼ 0.009] and menarche (crude OR 47.6; 95% CI 4.8 -472.9; P ¼ 0.001). Serum AMH correlated negatively with FSH and LH, but did not correlate with E2 and inhibin B. GH therapy increased the odds of having measurable AMH in TS (adjusted OR 4.1; 95% CI 1.9 -8.8; P , 0.001).limitations, reasons for caution: The cross-sectional design of the study does not allow longitudinal interpretation of the data; for that further studies are needed. High percentage of non-measurable AMH levels in the cohort of TS require categorized analysis.wider implications of the findings: Serum AMH levels are a useful marker of the follicle pool and thus ovarian function in pediatric patients with TS. These findings are in line with the published literature. The finding that GH therapy may affect AMH levels is novel, but must be confirmed by future longitudinal studies.

show abstract

“…One girl included had only three blood samples. Other aspects from this study have previously been published [10,11,12]. …”

Section: Methodsmentioning

confidence: 99%

Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition

Mouritsen

Søeborg²,

Johannsen³

et al. 2014

Horm Res Paediatr

View full text Add to dashboard Cite

Background: Accurate and selective assessment of testosterone requires use of a sensitive LC-MS/MS method, especially at low levels as those seen in young children. Methods: The present longitudinal study of 20 healthy children from the Copenhagen Puberty Study followed every 6 months for 5 years evaluates the longitudinal increase in serum testosterone before, during and after pubertal onset quantified by a newly developed LC-MS/MS method in comparison with immunoassay. Testosterone concentrations in serum samples (n = 177) were determined by LC-MS/MS (detection limit 0.1 nmol/l) and by immunoassay (detection limit 0.23 nmol/l). Results: Serum concentrations of testosterone increased gradually with age by both methods. However, serum testosterone was quantifiable in 9/10 girls prior to pubic hair development measured with LC-MS/MS, and in 2/10 girls measured with immunoassay. In boys, testosterone was quantifiable in 10/10 boys 1 year prior to pubic hair development measured with LC-MS/MS, and only in 1/10 boys measured with immunoassay. Serum testosterone levels were quantifiable 1.5 years (range 0.5-2.5) earlier using LC-MS/MS. Conclusion: Assessment of longitudinal circulating levels of serum testosterone using a selective LC-MS/MS method proved to be more sensitive in predicting early peripubertal changes in healthy children compared to levels determined by immunoassay.

show abstract

Individual serum levels of anti-Mullerian hormone in healthy girls persist through childhood and adolescence: a longitudinal cohort study

Cited by 120 publications

References 29 publications

The influence of antenatal exposure to phthalates on subsequent female reproductive development in adolescence: a pilot study

The influence of antenatal exposure to phthalates on subsequent female reproductive development in adolescence: a pilot study

Anti-Mullerian hormone levels in girls and adolescents with Turner syndrome are related to karyotype, pubertal development and growth hormone treatment

Longitudinal Changes in Circulating Testosterone Levels Determined by LC-MS/MS and by a Commercially Available Radioimmunoassay in Healthy Girls and Boys during the Pubertal Transition

Contact Info

Product

Resources

About