Individual effects of different selenocompounds on the hepatic proteome and energy metabolism of mice

Lennicke, Claudia; Rahn, Jette; Kipp, Anna P.; Dojčinović, Biljana; Müller, Andrea; Wessjohann, Ludger A.; Lichtenfels, Rudolf; Seliger, Barbara

doi:10.1016/j.bbagen.2016.08.015

Cited by 27 publications

(24 citation statements)

References 69 publications

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“…Recently, a significantly downregulation of GSTm1 and GSTp1 transcript and protein levels as well as GST enzyme activities were detected in liver tissues of the same mouse strain in response to Se supplementation when compared to the corresponding Se-deficient group [23], which was also demonstrated in rat liver tissues by Blum and coworkers [44]. Moreover, the mRNA level and enzyme activity of the Nrf2 target NAD(P)H dehydrogenase [quinone] 1 (NQO1) was significantly downregulated in the Se-supplemented groups when compared to the -Se group in liver [23]. However, these findings could not be observed in the colon (data not shown) indicating that there might be differences in the general redox-capacity between murine liver and colon.…”

Section: Discussionmentioning

confidence: 99%

“…In each group eight animals were randomly assigned. The groups were fed with diets supplemented with either adequate (ad; 150 μg Se/kg diet) or supra‐nutritive (high, hi; 750 μg Se/kg diet) Se concentrations in form of selenite, selenate, and SeMet, respectively, whereas the control group received a Se‐deficient diet (–Se) as previously described .…”

Section: Methodsmentioning

confidence: 99%

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Altered protein expression pattern in colon tissue of mice upon supplementation with distinct selenium compounds

et al. 2017

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The essential trace element selenium (Se) is controversially discussed concerning its role in health and disease. Its various physiological functions are largely mediated by Se incorporation in the catalytic center of selenoproteins. In order to gain insights into the impact of Se deficiency and of supplementation with different Se compounds (selenite, selenate, selenomethionine) at defined concentrations (recommended, 150 μg/kg diet; excessive, 750 μg/kg diet) in murine colon tissues, a 20-week feeding experiment was performed followed by analysis of the protein expression pattern of colon tissue specimens by 2D-DIGE and MALDI-TOF MS. Using this approach, 24 protein spots were identified to be significantly regulated by the different Se compounds. These included the antioxidant enzyme peroxiredoxin-5 (PRDX5), proteins with binding capabilities, such as cofilin-1 (COF1), calmodulin, and annexin A2 (ANXA2), and proteins involved in catalytic processes, such as 6-phosphogluconate dehydrogenase (6PGD). Furthermore, the Se compounds demonstrated a differential impact on the expression of the identified proteins. Selected target structures were validated by qPCR and Western blot which mainly confirmed the proteomic profiling data. Thus, novel Se-regulated proteins in colon tissues have been identified, which expand our understanding of the physiologic role of Se in colon tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Altered protein expression pattern in colon tissue of mice upon supplementation with distinct selenium compounds

et al. 2017

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“…It suggested the Se from SeNPs-CM is apt to be stored in the liver. Additionally, Se accumulation in the liver was found in mice or fish when BSA-SeNPs 34,44,45 or selenite 34 or selenate 46 were given. Possibly, inorganic Se was inclined to be collected in hepatic tissue.…”

Section: Selenium Retention In Vivomentioning

confidence: 99%

<p>Selenium Nanoparticles-Embedded Chitosan Microspheres and Their Effects Upon Alcohol-Induced Gastric Mucosal Injury in Rats: Rapid Preparation, Oral Delivery, and Gastroprotective Potential of Selenium Nanoparticles</p>

Bai¹,

Hong²,

Tan³

et al. 2020

IJN

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Background: Selenium (Se) is an indispensable trace element required for animals and human beings, whereas Se-deficiency can accelerate the development of acute gastric injury induced by over-consumption of alcohol. Selenium nanoparticles (SeNPs), as a special Sesupplement with favorable properties and unique bioactivities, are expected to play a passive role in gastroprotection. To the best of our knowledge, the gastroprotective potential of SeNPs is unknown and also, a rapid preparation of orally stable SeNPs available for prospective commercial application in the clinic is needed. Thus, SeNPs-embedded chitosan microspheres (SeNPs-CM) were developed to deliver SeNPs, and their gastroprotective potential was evaluated. Results: Herein, a rapid, eco-friendly and economic preparation process, composed of synthesis of SeNPs decorated by chitosan (CS), purification of CS-SeNPs by ultra-filtration (UF) and spray-drying of the purified CS-SeNPs, was introduced to prepare SeNPs-CM. The uniformly distributed SeNPs with a nanosize range of 60 nm were loaded into CSmicrospheres, and they could be released from the microspheres in gastric conditions. In addition, SeNPs-CM were safer than selenite in terms of Se dose, with a LD 50 of around 8-fold of that of selenite, and it could efficiently enhance the Se retention in Se-deficient Wistar rats. Furthermore, SeNPs-CM pre-treatment might significantly attenuate the ethanolinduced gastric mucosal damage, based on histological evaluation. It might be partly attributed to the systematic antioxidant activities of SeNPs-CM, reflected by the reduction in lipid peroxidation, the augmentation in antioxidant enzymatic activity as well as decreasing aggressive nitric oxides (NO). Conclusion: SeNPs-CM could be taken into consideration as a prospective Se-supplement for the oral delivery of SeNPs, with prominent gastroprotective effect against ethanolinduced mucosal injury.

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“…Although the significance of the applied Se species has been recognized as a key factor in health outcomes (reviewed in), only few studies have addressed species‐specific effects in vivo (e.g., refs. ). Therefore, the current study aimed for a comparative analysis of three different Se species including selenite, SeMet, and MeSeCys in the nematode Caenorhabditis elegans ( C. elegans ), examining their respective bioavailability, incorporation into proteins, protective role against oxidative stress, and impact on the antioxidative system, namely, TXNRD activity as well as the Nrf2/Skn‐1 and FoxO/Daf‐16 pathways.…”

Section: Introductionmentioning

confidence: 97%

Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems

Rohn

Raschke

Aschner

et al. 2019

Molecular Nutrition Food Res

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Scope: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro-and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. Methods and results: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. Conclusion: Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.

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Individual effects of different selenocompounds on the hepatic proteome and energy metabolism of mice

Abstract: Thus, it is important to consider Se compound-specific effects when supplementing with selenium.

Cited by 27 publications

References 69 publications

Altered protein expression pattern in colon tissue of mice upon supplementation with distinct selenium compounds

Altered protein expression pattern in colon tissue of mice upon supplementation with distinct selenium compounds

<p>Selenium Nanoparticles-Embedded Chitosan Microspheres and Their Effects Upon Alcohol-Induced Gastric Mucosal Injury in Rats: Rapid Preparation, Oral Delivery, and Gastroprotective Potential of Selenium Nanoparticles</p>

Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems

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