Organizational health helps companies achieve their goals, gain a competitive edge, and boost employee engagement and well-being by determining a company’s ability to establish, communicate, and implement a unified business strategy while retaining highly engaged employees. The current study aimed to develop and validate an original two-subscale measure that evaluates organizational health behavior determinants, including a quantitative 5-point Likert-type “Subscale A” of 16 items in 5 dimensions (awareness, appreciation, relations, employee engagement, and internal communication) and a qualitative “Subscale B” of 10 items in 3 dimensions (employee persona, organizational culture, and employee voice) called the Organization Health Behavior Index (OHBI). The combination of the two methodologies enhances the study’s credibility, validity, and applicability, as well as its contribution to the field of organizational health behavior research. Furthermore, the study included three sample groups and a sample of 3510 respondents from five sectors in the Kingdom of Saudi Arabia: agriculture, education, government, health, and logistics. This research includes various stages in the development and evaluation of the psychometric properties of the OHBI. These stages include conducting reliability analysis, performing exploratory and confirmatory factor analyses, and finalizing the scale. The model’s fit indices, such as the chi-squared test, comparative fit index (CFI), Tucker–Lewis index (TLI), root mean square error of approximation (RMSEA), and standardized root mean square residual (SRMR), collectively demonstrated a favorable fit for the model. The findings presented in this study offer support for the reliability and validity of the OHBI scale. The OHBI scale comprehensively encompasses the necessary psychometric properties related to its construction and validation, thereby establishing its validity and reliability as a robust measure of organizational health behavior. The OHBI can help create an environment at work that boosts productivity, engagement, and retention. Organizations can compare their health behavior ratings to industry standards or best practices using the index.