Indium chloride‐induced micronuclei via reactive oxygen species in Chinese hamster lung fibroblast V79 cells

Lin, Ruey-Hseng; Yang, Ming-Ling; Li, Yi-Ching; Chang, Hui-Min; Kuan, Yu‐Hsiang

doi:10.1002/tox.20755

Cited by 14 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Particleinduced cytotoxicity (with necrosis and/or apoptosis of the macrophage), which is much greater for InP compared with ITO, results in further release of solubilized (ionic) indium (together with other factors) extracellularly by the dying cells. This "free" ionic indium, which is much more toxic than particulate InP, may then in part drive pulmonary toxicity downstream via, for example, direct toxicity on adjacent macrophages, lung epithelial cells, and/or other cell types and/or indirectly via the induction of toxic-free radicals/reactive oxygen species causing oxidative stress (Chibli et al, 2011;Gottschling et al, 2001;Lin et al, 2013;Lison et al, 2009;Zurita et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Macrophage Solubilization and Cytotoxicity of Indium-Containing Particles as in vitro Correlates to Pulmonary Toxicity in vivo

Gwinn

Bousquet

et al. 2014

Toxicological Sciences

View full text Add to dashboard Cite

Macrophage-solubilized indium-containing particles (ICPs) were previously shown in vitro to be cytotoxic. In this study, we compared macrophage solubilization and cytotoxicity of indium phosphide (InP) and indium-tin oxide (ITO) with similar particle diameters (∼ 1.5 µm) and then determined if relative differences in these in vitro parameters correlated with pulmonary toxicity in vivo. RAW 264.7 macrophages were treated with InP or ITO particles and cytotoxicity was assayed at 24 h. Ionic indium was measured in 24 h culture supernatants. Macrophage cytotoxicity and particle solubilization in vitro were much greater for InP compared with ITO. To correlate changes in vivo, B6C3F1 mice were treated with InP or ITO by oropharyngeal aspiration. On Days 14 and 28, bronchoalveolar lavage (BAL) and pleural lavage (PL) fluids were collected and assayed for total leukocytes. Cell differentials, lactate dehydrogenase activity, and protein levels were also measured in BAL. All lavage parameters were greatly increased in mice treated with InP compared with ITO. These data suggest that macrophage solubilization and cytotoxicity of some ICPs in vitro are capable of predicting pulmonary toxicity in vivo. In addition, these differences in toxicity were observed despite the two particulate compounds containing similar amounts of indium suggesting that solubilization, not total indium content, better reflects the toxic potential of some ICPs. Soluble InCl3 was shown to be more cytotoxic than InP to macrophages and lung epithelial cells in vitro further suggesting that ionic indium is the primary cytotoxic component of InP.

show abstract

Section: Discussionmentioning

confidence: 99%

Macrophage Solubilization and Cytotoxicity of Indium-Containing Particles as in vitro Correlates to Pulmonary Toxicity in vivo

Gwinn

Bousquet

et al. 2014

Toxicological Sciences

View full text Add to dashboard Cite

show abstract

“…Viability of BV2 cells after treating with wogonin was determined by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay (Lin et al, ). BV2 cells were plated in 24‐well culture plates (2 × 10 5 cells/well), pre‐treated with different concentrations of wogonin (1, 3, 10, or 30 μM) for 18 h, then incubated with 0.5 mg/mL of MTT solution for 2 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Wogonin attenuates endotoxin‐induced prostaglandin E2 and nitric oxide production via Src‐ERK1/2‐NFκB pathway in BV‐2 microglial cells

Yeh

Yang

Lee

et al. 2013

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

Microglia are the major component of intrinsic brain immune system in neuroinflammation. Although wogonin expresses anti-inflammatory function in microglia, little is known about the molecular mechanisms of the protective effect of wogonin against microglia activation. The aim of this study was to evaluate how wogonin exerts its anti-inflammatory function in BV2 microglial cells after LPS/INFγ administration. Wogonin not only inhibited LPS/ INFγ-induced PGE2 and NO production without affecting cell viability but also exhibited parallel inhibition on LPS/INFγ-induced expression of iNOS and COX-2 in the same concentration range. While LPS/INFγ-induced expression of P-p65 and P-IκB was inhibited by wogonin-only weak inhibition on P-p38 and P-JNK were observed, whereas it significantly attenuated the P-ERK1/2 and its upstream activators P-MEK1/2 and P-Src in a parallel concentration-dependent manner. These results indicated that the blockade of PGE2 and NO production by wogonin in LPS/INFγ-stimulated BV2 cells is attributed mainly to interference in the Src-MEK1/2-ERK1/2-NFκB-signaling pathway.

show abstract

“…ITO also increased mutation in rat alveolar type II cells (Driscoll et al 1997) and carcinogenicity in male and female rats (Nagano et al 2011). Indium chloride also induced micronuclei formation (Takagi et al 2011;Lin et al 2011). Due the ability of the Comet assay to detect low levels of DNA damage in different cell types, the Comet assay represents a powerful tool with which to identify DNA damage.…”

Section: Resultsmentioning

confidence: 99%

Genotoxicity of indium tin oxide by Allium and Comet tests

et al. 2013

View full text Add to dashboard Cite

Genotoxic effects of indium tin oxide (ITO) were investigated on root cells of Allium cepa by employing both Allium and Comet assays. A. cepa roots were treated with the aqueous dispersions of ITO at 5 different concentrations (12.5, 25, 50, 75, and 100 ppm) for 4 h. Exposure of ITO significantly increased mitotic index, and total chromosomal aberrations by the Allium test. While chromosome laggards, stickiness, disturbed anaphase-telophase and anaphase bridges were observed in anaphase-telophase cells, c-metaphase and binuclear cells were observed in other cells. A significant increase in DNA damage was also observed at all concentrations of ITO by the Comet assay. These results indicate that ITO exhibits genotoxic activity in A. cepa root meristematic cells.

show abstract

Indium chloride‐induced micronuclei via reactive oxygen species in Chinese hamster lung fibroblast V79 cells

Cited by 14 publications

References 28 publications

Macrophage Solubilization and Cytotoxicity of Indium-Containing Particles as in vitro Correlates to Pulmonary Toxicity in vivo

Macrophage Solubilization and Cytotoxicity of Indium-Containing Particles as in vitro Correlates to Pulmonary Toxicity in vivo

Wogonin attenuates endotoxin‐induced prostaglandin E2 and nitric oxide production via Src‐ERK1/2‐NFκB pathway in BV‐2 microglial cells

Genotoxicity of indium tin oxide by Allium and Comet tests

Contact Info

Product

Resources

About