Indirect Spectrophotometric Determination of

Abstract

Simple, reliable and sensitive indirect spectrophotometric method was developed for the determination of cephalexin in pharmaceutical products without pretreatment. The method was based on the fading effect of the cephalexin on developed color product resulted from the bromination reaction of NBS with p-anisidine in acidic medium, subsequently vanished colored species was measured at 522 nm. Cephalexin acts in accordance with the Beer's law, under optimum conditions, at concentration ranges 1.5-10.0 µg/mL (R = 0.9949) with detection limit of 0.90 µg/mL. The relative standard deviation and error percent for 3 replicate determinations were found to be 2.2% and 1.75%, respectively. The proposed method was applied to the determination of cephalexin in different pharmaceutical products. A comparison of the method reported is made with standard official method and can be used as a convenient analysis method for routine quality control.