Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’

Delgado-Aceves, Lourdes; González-Arnao, María Teresa; Santacruz-Ruvalcaba, Fernando; Folgado, Raquel; Portillo, Liberato

doi:10.3390/plants10020249

Cited by 12 publications

(6 citation statements)

References 48 publications

(61 reference statements)

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“…Previous studies have shown that the regrowth and conversion rates after cryopreservation were directly affected by the time of exposure to cryoprotectant treatment and the kind of cryoprotectant used. Delgado-Aceves et al [ 39 ] revealed that the regrowth and conversion of Agave somatic embryos achieved higher rates after exposure for 15 and 30 min in a plant vitrification solution (composed of glycerol, DMSO, ethylene glycol, and sucrose) when compared to exposures of 45 and 60 min, suggesting that lower incubation times in pretreatment solution may result in major recovery and conversion rates. Salaj et al [ 40 ] indicated that the regrowth rate after cryopreservation of ECs of different cell lines of P. nigra were affected by the pre-treatment used, in which sorbitol and maltose pre-treatment resulted in less regrowth than sucrose and cryopreservation did not affect the conversion and proliferation of cultures.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Antioxidant System Activation Assures the Viability of Guadua chacoensis (Bambusoideae, Poaceae) Embryogenic Cultures during Cryopreservation

Polesi

Goeten

Fraga

et al. 2023

Plants

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This study aimed to establish a cryopreservation protocol for G. chacoensis embryogenic cultures (ECs) and to investigate the role of antioxidant enzymes activities during cryopreservation. The growth dynamics of cell suspensions were also investigated, followed by a phytotoxicity test to assess the ECs’ ability to tolerate the use of cryoprotective solutions for different incubation times (0, 30, 60, 120, and 240 min). We evaluated the EC redox state in three steps of cryopreservation: after incubation in cryoprotection solution, after thawing, and 60 days after regrowth. Our results showed that the ECs support the use of cryoprotective solution until 120 min, showing phytotoxic effects with 240 min of incubation. This study reports a 100% survival of the cultures and a 10% increase ratio in fresh material for both incubation times tested (60 and 120 min). Increased malonaldehyde content was identified after incubation in the cryoprotective solution. An increase in the activities of catalase and ascorbate peroxidase was also identified in the subsequent steps, suggesting that the activation of antioxidant enzymes is essential for maintaining cell homeostasis during cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Enzymatic Antioxidant System Activation Assures the Viability of Guadua chacoensis (Bambusoideae, Poaceae) Embryogenic Cultures during Cryopreservation

Polesi

Goeten

Fraga

et al. 2023

Plants

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“…Previous studies also indicated that plant vitrification solutions are used in the cryoprotection process, and most previous studies also indicated that PVS2 was Sustainability 2023, 15, 14218 9 of 13 the best vitrification solution [15,51,52]. This is because it has a cryoprotection material that is not lethal to most plant tissues, and the most used and successful PVS2 concentration was 100% in different studies [15,[52][53][54]. Furthermore, the duration of incubation in PVS2 was documented in many studies as 30-50 min, and this was critical in many species [55].…”

Section: Cryopreservation Of S Dominica Callusmentioning

confidence: 99%

A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

Al-Qudah,

Shibli,

Zatimeh

et al. 2023

Sustainability

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Salvia dominica L. is an important wild medicinal plant that grows in Jordan and neighboring countries, and this plant has been suffering from many threats in its wild environment. Therefore, this research aims to establish a comprehensive and sustainable approach via an in vitro propagation and conservation system for the S. dominica L. plant. Axillary buds were used to initiate the in vitro culture on Murashige and Skoog MS media supplemented with 0.5 mg L−1 of GA3. In vitro shoot proliferation and rooting were experimented on with different concentrations of cytokinins and auxins, respectively. Calli were induced in the dark on excised leaf discs (0.5 cm in diameter), and multiplication was experimented on with different growth regulators. Cryopreservation experiments were applied on the callused segments under different growth conditions via the vitrification technique. A full protocol was achieved for shoot proliferation with 6.3 shoots/explant using 1.2 mg L−1 of thidiazuron (TDZ), while rooting was achieved at 1.5 mg L−1 of NAA with 6.6 functional roots/explant. Acclimatization was completely successful for the rooted plants. The highest callus production with 5.81 g/calli was achieved using 1.5 mg L−1 of benzylaminopurine (BAP). Cryopreservation of the S. dominica calli was successfully achieved when a pure plant vitrification solution (PVS2) was used to dehydrate the calli for 20 min after immersion in the loading solution for 20 min with a 76.6% regrowth percentage. The loading and the plant vitrification solution type and duration were the most critical points in the regrowth of the cryopreserved calli. In conclusion, a successful protocol was set up for the in vitro propagation and conservation of S. dominica calli. This study has prompted us to perform further studies on sustainable in vitro production and conservation of critically endangered medicinal plants to implement a green environment protecting against surrounding threats.

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“…A histological analysis of the chronological sequence of morphological events during ontogeny is a way to know what morphogenic process was induced (Delporte et al, 2014). Somatic embryogenesis and/or organogenesis has been described and confirmed using histological analysis in different species (Delgado-Aceves et al, 2021;Mamgain et al, 2022;García-Hernández et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

López-Puc¹,

Góngora²,

Cano-Sosa³

et al. 2023

Trop Subtrop Agroecosyst

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Background: Chrysanthemum is one of the most popular landscape plants worldwide. Introducing desirable traits into this plant by conventional breeding has limitations due to cross-incompatibility. An approach to this problem is the introduction of resistance or tolerance traits via plant genetic transformation. In vitro regeneration of plants is necessary for implementing genetic transformation systems; therefore, regeneration via morphogenesis is required before any genetic transformation attempt. Objective: To induce morphogenesis from the leaf explant of Dendranthema grandiflora var Micromargara. Methodology: Micropropagation from node cuttings was induced from node cuttings of 3 cm in length from 6 months-old plants that were used as explants to produce seedlings as source explants. Morphogenesis experiments were done using leaf segments of 1 cm2 from 8-weeks-old in vitro seedlings. The explants were transferred to basal medium Murashige and Skoog at 4.4 gL-1 supplemented with different plant growth regulators. During the morphogenic process, leaves samples were collected to detect which morphogenic process was induced. Results: Node cuttings culture on Murashige and Skoog medium at 3.3 gL-1 supplemented with benzylaminopurine at 2.2 µM yielded a vegetative growth increase from 1 to 7 shoots, 2 to 12 leaves per node cutting, and the stem length of 3 to 9.5 cm. The leaf explant induced three ways of in vitro morphogenesis: direct and indirect roots organogenesis (rhizogenesis) and, shoot organogenesis. Direct rhizogenesis was induced in plant growth regulator-free Murashige and Skoog medium (18 roots/explant). Indirect rhizogenesis from leaf explant was less efficient than direct rhizogenesis, get obtaining six roots per leaf explant cultured on Murashige and Skoog medium at 4.4 g L-1 supplemented with 0.4 µM thidiazuron and 4.5 µM 2,4-dichlorophenoxyacetic acid, and three roots per leaf explant cultured on Murashige and Skoog medium supplemented with 0.4 µM thidiazuron and 9.05 µM 2,4-dichlorophenoxyacetic acid. Indirect shoot organogenesis was induced from leaf explants cultured on Murashige and Skoog at 4.4 g L-1 supplemented with 13.32 µM benzylaminopurine and 4.83 µM naphthalene acetic acid; 50% of explants with callus formed shoots (2 shoots/leaf explant). Through histological analysis it was possible to verify that the morphogenic response obtained was organogenesis. Implications: Regeneration of Dendranthema grandiflora var. Micromargara established herein will allow improvement of the variety through techniques of genetic transformation mediated by Agrobacterium and any other strategy to incorporate or silence genes of interest in the plant. Conclusion: The high capacity to induce roots of Dendranthema grandiflora var Micromargara, may provide an efficient model for further investigating the mechanism of rhizogenesis from leaf explants cultured without plant growth regulators.

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Indirect Somatic Embryogenesis and Cryopreservation of Agave tequilana Weber Cultivar ‘Chato’

Cited by 12 publications

References 48 publications

Enzymatic Antioxidant System Activation Assures the Viability of Guadua chacoensis (Bambusoideae, Poaceae) Embryogenic Cultures during Cryopreservation

Enzymatic Antioxidant System Activation Assures the Viability of Guadua chacoensis (Bambusoideae, Poaceae) Embryogenic Cultures during Cryopreservation

A Sustainable Approach to In Vitro Propagation and Conservation of Salvia dominica L.: A Wild Medicinal Plant from Jordan

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

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