Indirect Sequential Injection Enzymatic Determination of Allopurinol in Pharmaceuticals Based on Xanthine Oxidase Inhibition

Silvestre, Carlos; Segundo, Marcela A.; Saraiva, M. Lúcia M.F.S.; Lima, José L.F.C.

doi:10.1080/00387010903185538

Cited by 3 publications

(1 citation statement)

References 26 publications

(37 reference statements)

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“…High sensitivity SIA is performed in SFC mode, when the reaction product is arrested in the flow cell, yielding a reaction rate curve. The advantage of the SHC protocol is speed and simplicity, while the SFC protocol offers higher sensitivity and is free from the influence of the refractive index and the background color interference [ 15 , 27 – 29 ].…”

Section: Introductionmentioning

confidence: 99%

Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate

Pliego

Mateos

Rodríguez

et al. 2015

Sensors

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Lipases and esterases are biocatalysts used at the laboratory and industrial level. To obtain the maximum yield in a bioprocess, it is important to measure key variables, such as enzymatic activity. The conventional method for monitoring hydrolytic activity is to take out a sample from the bioreactor to be analyzed off-line at the laboratory. The disadvantage of this approach is the long time required to recover the information from the process, hindering the possibility to develop control systems. New strategies to monitor lipase/esterase activity are necessary. In this context and in the first approach, we proposed a lab-made sequential injection analysis system to analyze off-line samples from shake flasks. Lipase/esterase activity was determined using p-nitrophenyl butyrate as the substrate. The sequential injection analysis allowed us to measure the hydrolytic activity from a sample without dilution in a linear range from 0.05–1.60 U/mL, with the capability to reach sample dilutions up to 1000 times, a sampling frequency of five samples/h, with a kinetic reaction of 5 min and a relative standard deviation of 8.75%. The results are promising to monitor lipase/esterase activity in real time, in which optimization and control strategies can be designed.

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Section: Introductionmentioning

confidence: 99%

Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate

Pliego

Mateos

Rodríguez

et al. 2015

Sensors

View full text Add to dashboard Cite

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Current Awareness in Drug Testing and Analysis

2010

Drug Testing and Analysis

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Bhushan R, Kumar R// Indian Inst Technol, Dept Chem, IN-247667 Roorkee, IndiaBiomed Chromatogr 2010 24 (1) 66 Enantioresolution of dl-penicillamine Penicillamine (PenA) is a nonproteinogenic amino acid containing a thiol group. The three functional groups in penicillamine undergo characteristic chemical reactions and differ in their ability to participate in various chemical and biochemical reactions. D-penicillamine is more active pharmacologically, while the L-isomer occurs 'naturally'. This review deals with the enantioresolution of PenA both by direct and indirect methods using liquid chromatography. HPLC separation of its diastereomers prepared with different chiral derivatizing reagents (on reversed-phase columns) and separation of the derivatives prepared with achiral reagents (on chiral columns or via ligand exchange mode) has been discussed. Separation of enantiomers tagged with achiral reagent (to add a chromophore for ehanced detection) when there is no diastereomer formation has been considered separately. In addition, application of PenA and its derivatives as chiral selector for enentioresolution of certain other compounds has also been discussed Daeid NN, Buchanan HAS, Savage KA, Fraser JG, Cresswell SL// Univ Strathclyde, Dept Pure & Appl Chem, Ctr Forensic Sci, Glasgow G1 1XW, Scotland Aust J Chem 2010 63 (1) 3 Recent advances in the application of stable isotope ratio analysis in forensic chemistryThis review paper updates the previous literature in respect of the continued and developing use of stable isotope ratio analysis in samples which are relevant to forensic science. Recent advances in the analysis of drug samples, explosive materials, and specimens obtained from human and animal samples are discribed. The paper also addresses the use of isotope ratio mass spectrometry in a forensic context and discuss its evidential potential Kataoka H// Shujitsu Univ, Sch Pharm, Okayama 703 8516, Japan Anal Bioanal Chem 2010 396 (1) 339 Recent developments and applications of microextraction techniques in drug analysisSample preparation is crucial for isolating desired components from complex matrices and greatly afects their reliable and accurate analysis. Recent developments in sample preparation include miniaturization, automation, high-throughput performance, online coupling with analytical instruments, and low-cost operation through extremely low or no solvent consumption. Microextraction procedures, for example liquid-phase microextraction and solid-phase microextraction, incorporate these advantages when compared with the traditional approaches of liquid-liquid extraction and conventional solid-phase extraction. This review highlights microextraction techniques produced over the last decade and presents a synopsis of the attributes of various approaches in drug analysis Anal Bioanal Chem 2010 396 (1) 379 Biological matrices for the evaluation of exposure to environmental tobacco smoke during prenatal life and childhood Biomarker assessment of the exposure to environmental tobacco smoke during ch...

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