Experimental
Reagents and materialsReagents employed were of guaranteed reagent grade and were obtained from Wako Pure Chemical Industries (Osaka, Japan), unless otherwise noted. N- 7,10,13,16,19,22,25,28,31, and N-hydroxysuccinimido-12-methoxy-4,7,10-trioxadodecanoate (Methyl-4OE-NHS ester) were obtained from PIERCE (Rockford, IL). SUNBRIGHT ME-020AS (Methyl-POE-2000-NHS ester) and SUNBRIGHT ME-050AS (Methyl-POE-5000-NHS ester) were obtained from NOF Corporation (Tokyo, Japan), and the average molecular