INDIRECT ORGANOGENESIS AND ESTIMATION OF NUCLEAR DNA CONTENT IN REGENERATED CLONES OF A NON-TOXIC VARIETY OF Jatropha curcas

Abstract

<p class="Abstract"><em>Jatropha curcas</em> L. is a second-generation energy crop, which produces approximately 40% oil in its seeds, which can be transformed into biodiesel. <em>In vitro</em> culture is a valuable tool for the multiplication and conservation of elite plant varieties. In <em>J. curcas</em>, there are several reports on the micropropagation of the species, but with low reproducibility. The objective of this study was to obtain the <em>in vitro</em> organogenesis of <em>J. curcas</em> and estimate the nuclear DNA content by flow cytometry during eight subcultures <em>in vitro</em>. The organogenesis of adventitious shoots was obtained in 3.3 g/l of MS, 110.25 μM of 6–(γ, γ-Dimethylallylamino) purine (2ip), 1.27 μM of indoleacetic acid (IAA) and 369.21 μM of adenine sulfate (AdS), obtaining up to 18.50 ± 0.7 shoots per explant. The development of the shoots was 1.67 ± 0.76 cm in MS medium, 4.44 μM of benzylaminopurine (BAP), 1.0 μM of IAA and 543 μM of AdS. The rooting was 13.6 ± 2.51 roots in ½ MS and 14.7 μM of indole butyric acid (IBA). The nuclear DNA content was from 0.80 ± 0.12 to 1.07 ± 0.23 pg of nuclear DNA during the eight subcultures.</p>