Indirect Immunofluorescence Assay for Rapid Diagnosis of Influenza Virus

Guenthner, Sharon H.; Linnemann, Calvin C.

doi:10.1093/labmed/19.9.581

Cited by 9 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conventional cell cultures detected 45 influenza viruses, with a mean detection time of 4 days. Similar results with centrifuged Madden-Darby canine kidney (MDCK) cell cultures and influenza virus detection were reported by Guenthner and Linnemann (111). However, Stokes et al (274) and Bartholoma and Forbes (17) reported sensitivities of 84 to 91% for centrifuged influenza cultures stained at 16 to 18 h postinfection.…”

Section: Centrifugationsupporting

confidence: 77%

See 1 more Smart Citation

Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Hughes¹

1993

Clinical Microbiology Reviews

View full text Add to dashboard Cite

Section: Centrifugationsupporting

confidence: 77%

“…For example, Stokes et al (274) reported using trypsin with their MDCK cells, while Guenthner and Linnemann did not (111).…”

Section: Centrifugationmentioning

confidence: 99%

Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Hughes¹

1993

Clinical Microbiology Reviews

View full text Add to dashboard Cite

“…Oneor 2-day shell vial assays detect 73% of RSV isolates (10), 77 to 97% of adenovirus isolates (5, 13), and 55 to 84% DETECTION OF RESPIRATORY VIRUSES BY SHELL VIAL ASSAY of influenza A and B virus isolates (1,6,9,12,19). This use of the Baxter-Bartels monoclonal antibody pool in a 2-day shell vial assay and its comparison to cell culture with results similar to ours has been reported in abstract form only (17,20).…”

Section: Discussionmentioning

confidence: 99%

Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool

et al. 1992

View full text Add to dashboard Cite

We compared the detection of seven respiratory viruses by using a commercially available monoclonal antibody pool in a 2-day shell vial assay with that by using standard cell culture with respiratory syncytial virus (RSV) enzyme-linked immunosorbent assay (ELISA)-negative nasal secretions from hospitalized children. We found 179 respiratory virus isolates by either method in 675 specimens. Overall, the shell vial assay detected 147 of 179 (79%v) of the positives after 2 days; cell culture detected 148 of 179 (80%o) after a mean incubation period of 7.6 days (range, 1 to 14 days). The sensitivity of the shell vial assay was 78% for RSV, 94% for influenza B virus, 83% for adenovirus, and 80% for parainfluenza viruses. The sensitivity of the cell culture was 70% for RSV, 79%o for influenza B virus, 90%o for adenovirus, and 89%, for parainfluenza viruses. The 2-day shell vial assay allowed the detection of respiratory viruses in a clinically relevant time frame and rapidly detected RSV in specimens lacking RSV antigen by ELISA.

show abstract

Diagnosis of Viral Respiratory Tract Infections

Myint¹

1996

Viral and Other Infections of the Human Respiratory Tract

View full text Add to dashboard Cite

Indirect Immunofluorescence Assay for Rapid Diagnosis of Influenza Virus

Cited by 9 publications

References 0 publications

Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool

Diagnosis of Viral Respiratory Tract Infections

Contact Info

Product

Resources

About