Indirect detection in reversed-phase liquid chromatography: Ion-pair retention models for ionic compounds on μ Bondapak phenyl

Arvidsson, E.; Hackzell, L.; Schill, G; Westerlund, Douglas

doi:10.1007/bf02324787

Cited by 18 publications

(5 citation statements)

References 11 publications

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“…The effects seen in Figure are consistent with the behavior previously observed for sample and system peaks during indirect UV detection elution chromatography of an ionic sample employing a probe having the same sign of charge. − ,− , In fact, plots similar to those in Figure have previously been reported for other cases in which sample and probe ion have the same charge. ,,,,,,, It is the purpose of the present study to discover the cause of this phenomenon.…”

Section: Resultssupporting

confidence: 87%

“…[1][2][3][5][6][7][8][9][10][11][12][13][14]17 In fact, plots similar to those in Figure 1 have previously been reported for other cases in which sample and probe ion have the same charge. 3,5,6,9,13,14,17,25 It is the purpose of the present study to discover the cause of this phenomenon.…”

Section: Resultssupporting

confidence: 68%

“…The presently reported study deals with indirect UV detection of an ionic sample using an ionic probe having the same sign of charge as the sample. The pattern of eluted sample and system peaks that is observed for this case reveals that there is a local decrease in the amount of probe sorbed in the sample zone. , A number of physicochemical retention models have been proposed in the literature to explain this decreased sorption of probe ion in the presence of sample ion. − …”

mentioning

confidence: 79%

“…) exp ( -µ°N BTA,AD + Z + Fψ I RT ) (9) n i,DL ) the experimentally measured values reflect the loss of i + from the diffuse layer.…”

Section: Theorymentioning

confidence: 93%

“…3,5 A number of physicochemical retention models have been proposed in the literature to explain this decreased sorption of probe ion in the presence of sample ion. [5][6][7][8][9][10][11][12][13][14][15][16] Some of these models invoke an electrostatic repulsion effect and may or may not also invoke competition for space between the sample and probe species. 5,[14][15][16][17][18] Most of the models are formulated in terms of the "ion interaction" theory, in which the electrical potential at the interface between the reversed-phase bonded phase (RPBP) and the mobile phase does not appear explicitly.…”

mentioning

confidence: 99%

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Electrical Double-Layer Effects and the Origin of Indirect UV Detection in the Liquid Chromatography of a Cationic Sample Using a Cationic Probe

Glavina

Cantwell

1996

Anal. Chem.

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Two cations, tetra-n-butylammonium (TBA(+)) and (4-nitrobenzyl)trimethylammonium (NBTA(+)), are simultaneously sorbed on the bonded phase sorbent Partisil-10 ODS-3 from water at various ionic strengths. The non-UV-absorber TBA(+) is thought of as the sample ion and the UV-absorber NBTA(+) as the probe ion in an indirect detection system. In the sorbed state, the two ions reside at different charge planes: NBTA(+) is very close to the ODS-water interface, and TBA(+) is about 11 Å deeper into the ODS phase. Both ions contribute to the electrical potential ψ(I) at the NBTA(+) charge plane, which can be calculated from an electrical triple-layer model. The sorption of TBA(+) influences the amount of NBTA(+) sorbed both by raising ψ(I) and by altering the amount of space available for NBTA(+). The former effect is greater than the latter at ionic strengths up to at least 0.5 mol/L. For the latter effect, the direction and magnitude of the influence of TBA(+) on available space both change with ionic strength (c). At low c, sorbed TBA(+) increases the amount of space available for NBTA(+) by disrupting the conformation of the alkyl chains of the ODS phase. At high c, sorbed TBA(+) decreases the amount of space available by competing with NBTA(+) for space. This dramatically different effect of TBA(+) on available space at low and high c occurs because higher c produces both a weaker interaction of the ODS alkyl chains with water and a reorientation of NBTA(+) from flat to perpendicular in the ODS phase.

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