Indirect analysis of corticosteroids. 2. The determination and identification of urinary 17-hydroxy-20-oxosteroids unsubstituted at C(21)

Catecholamine excretion, cholesterol, triglycerides, free fatty acids (FFA) and plasma 11-OH corticoids were measured in 18 normal workers of an aircraft factory exposed to turbine noise for 3 h. Blood pressure and pulse frequency were also registered. In 72% of the subjects, marked elevations of catecholamine excretion were found, correlating fairly well with increases of cholesterol, FFA, blood pressure and pulse frequency. Surprisingly, after 3 h of noise exposure, the plasma 11-OH corticoid mean value was slightly lower than the one of the early morning in resting conditions; this could be ascribed to the pronounced midday drop of plasma corticoids, characteristic of the circadian cortisol variation. This could counteract the moderate corticoadrenal stimulation caused by the noise stress. The blood pressure and plasma lipid elevation found in these workers might be detrimental to personnel suffering from atherosclerosis or other vascular pathology.

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“…Urinary 17-ketosteroids were determined with R icca's method [18], and 17-hydroxycorticoids with the method of A ppleby and Norymberski [2].…”

Section: Methodsmentioning

confidence: 99%

Modifications of Epinephrine, Norepinephrine, Blood Lipid Fractions and the Cardiovascular System Produced by Noise in an Industrial Medium

Ortiz¹,

Argüelles²,

Crespin³

et al. 1974

Horm Res

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“…Table 2 represents recrystallization data for products obtained from incubations of interstitial and seminiferous tubule tissue. 17a,20a-Dihydroxyprogesterone was subjected to oxidation with sodium bismuthate (Appleby & Norymberski, 1955) Determination of radioactivity After identification, steroids were evaporated into vials and 10 ml toluene containing 4 g 2,5 diphenyl oxazole and 50 mg l,4-di-[2-(5-phenyl oxazolyl)] benzene per litre were added. The radio¬ active content of these steroids was estimated by liquid scintillation spectroscopy in a Packard Tri-Carb Liquid Scintillation Spectrometer, Model 3380, adjusted for double isotope counting.…”

Section: Recrystallization Ofproductsmentioning

confidence: 99%

Correlation between ultrastructure and biochemical changes in the testis of the American grey squirrel, Sciurus carolinensis, during the reproductive cycle

Pudney¹,

Lacy²

1977

Reproduction

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Summary. Distinct differences in the ability of isolated seminiferous tubules and interstitium to utilize steroid precursors for androgen production in vitro were observed during the reproductive cycle of the American grey squirrel, Sciurus carolinensis. In spermatogenically active testes, the seminiferous tubules and the interstitium readily synthesized androgens from labelled C21 steroid precursor; seminiferous tubules also produced significant amounts of 17\g=a\,20\g=a\-dihydroxyprogesterone.However, during sexual regression androgen synthesis was drastically reduced in both testicular components, while the production of 20\g=a\-reducedmetabolites of progesterone and 17\g=a\-hydroxyprogesterone was increased. Many of the fine-structural changes occurring in Leydig and Sertoli cells during the reproductive cycle could be correlated with the capacity of isolated seminiferous tubules and interstitium to formulate androgens from labelled precursors.

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“…Total neutral 17-ketosteroids were determined by the method of Norymberski, Stubbs & West (1953), and 17-hydroxycorticosteroids by the method of Appleby & Norymberski (1955). Noradrenaline and adrenaline were estimated by the method of Euler & Lishajko (1959), modified by using lyophilization for concentration and by using a spectrophoto-fluorometer for estimation; reproducibility and recoveries were of the same order as those of the original workers.…”

Section: Methodsmentioning

confidence: 98%

Urinary excretion of adrenal steroids, catecholamines and electrolytes in man, before and after acclimatization to cold in Antarctica

Budd¹,

Warhaft²

1970

The Journal of Physiology

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Indirect analysis of corticosteroids. 2. The determination and identification of urinary 17-hydroxy-20-oxosteroids unsubstituted at C(21)

Cited by 49 publications

References 11 publications

Modifications of Epinephrine, Norepinephrine, Blood Lipid Fractions and the Cardiovascular System Produced by Noise in an Industrial Medium

Modifications of Epinephrine, Norepinephrine, Blood Lipid Fractions and the Cardiovascular System Produced by Noise in an Industrial Medium

Correlation between ultrastructure and biochemical changes in the testis of the American grey squirrel, Sciurus carolinensis, during the reproductive cycle

Urinary excretion of adrenal steroids, catecholamines and electrolytes in man, before and after acclimatization to cold in Antarctica

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