Indigofera linifolia ameliorated CCl4 induced endoplasmic reticulum stress in liver of rat

Naz, Irum; Khan, Muhammad Rashid; Zai, Jawaid Ahmed; Batool, Riffat; Maryam, Sonia; Majid, Muhammad

doi:10.1016/j.jep.2021.114826

Cited by 4 publications

(11 citation statements)

References 54 publications

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“…In our current results, lower expression levels of Nrf2, PDI, and GCLC were found in the liver homogenate after CCl 4 treatment, indicating liver injuries, ER stress, and hepatotoxicity. Our results are similar to those of previous studies. , However, significant increases in the expression levels of these antioxidant markers were found after P. candollei administration.…”

Section: Discussionsupporting

confidence: 92%

“…2 Our study also showed that the levels of these cytokines (TNF-α, IL-6, and MCP-1) were upregulated in CCl 4-intoxicated rats, while the expression of these markers was restored in PCM-treated rats, especially at a high dose of 600 mg/kg. This finding is similar to the previous study of Naz et al 34 The TGF-β1/Smad signaling pathway is a key indicator of liver fibrosis. Overexpression of TGF-β1 is an attribute of damage to liver cells and liver fibrosis.…”

Section: Acssupporting

confidence: 93%

“…The presence of phytochemicals such as phenolics and vanillin in plant extracts is associated with their high antioxidant activities. Studies have found that plant extracts with high TPC and TFC levels have the potency to quench free radicals including DPPH and exhibit reducing power activities . Moreover, Apiaceae extracts typically contain polyphenolic compounds, including flavonoids, tannins, and phenolic acids.…”

Section: Discussionmentioning

confidence: 99%

“…Our results are similar to those of previous studies. 34,39 However, significant increases in the expression levels of these antioxidant markers were found after P. candollei administration. Furthermore, prior to clinical trials and dose selection for humans, the determination of safety and toxicity profile of extract in humans is mandatory.…”

Section: Acsmentioning

confidence: 96%

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Pleurospermum candollei Methanolic Extract Ameliorates CCl₄-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats

et al. 2023

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The main objective of this study was to investigate the hepatoprotective potency of the Pleurospermum candollei methanol extract against CCl4-induced liver damage in rats. HPLC technique was used to estimate the presence of polyphenols in the methanol extract of P. candollei (PCM), while proximate analysis revealed the presence of carbohydrates, lipids, and moisture in the extract. The antioxidant potential of PCM was evaluated by 2,2-diphenylpicrylhydrazyl (DPPH) and reducing power assay, which showed a high percentage of inhibition against free radicals. Hepatotoxicity was induced by carbon tetrachloride (CCl4). CCl4 administration reduced the activity of endogenous antioxidants, whereas it increased the production of nitrites and hydrogen peroxide (H2O2) in rats. Furthermore, the level of hepatic markers in serum was also elevated after CCl4 administration. Moreover, the expression of stress-related markers, proinflammatory mediators, and apoptotic genes was enhanced in CCl4-treated rats. Coadministration of PCM along with CCl4 in rats reduced the levels of free radicals and the above genes to normal levels. CCl4 administration caused histopathological alterations in liver tissues, while cotreatment with PCM mitigated liver injuries. These findings suggest that the methanol extract of P. candollei possesses antioxidant and anti-inflammatory properties and can prevent liver injury. Further pharmacological research will be helpful in determining the effectiveness of P. candollei in humans. Development of FDA-approved plant-based anti-inflammatory drugs can help treat patients and reduce the chances of toxicity.

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Section: Discussionsupporting

confidence: 92%

Section: Acssupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Acsmentioning

confidence: 96%

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Pleurospermum candollei Methanolic Extract Ameliorates CCl₄-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats

et al. 2023

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“…Rats of both sexes were divided into 8 experimental groups and each group which comprised of 6 rats that were randomly divided at a ratio of 1:1 in each experimental group. The body weight of all animals ranged between (120 g–180 g) [ 78 , 79 ] and they ranged from 4–6 weeks old ( Figure 1 A). All animals were fed on normal feed and water.…”

Section: Methodsmentioning

confidence: 99%

N-Acetyl Cysteine, Selenium, and Ascorbic Acid Rescue Diabetic Cardiac Hypertrophy via Mitochondrial-Associated Redox Regulators

et al. 2021

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Metabolic disorders often lead to cardiac complications. Metabolic deregulations during diabetic conditions are linked to mitochondrial dysfunctions, which are the key contributing factors in cardiac hypertrophy. However, the underlying mechanisms involved in diabetes-induced cardiac hypertrophy are poorly understood. In the current study, we initially established a diabetic rat model by alloxan-administration, which was validated by peripheral glucose measurement. Diabetic rats displayed myocardial stiffness and fibrosis, changes in heart weight/body weight, heart weight/tibia length ratios, and enhanced size of myocytes, which altogether demonstrated the establishment of diabetic cardiac hypertrophy (DCH). Furthermore, we examined the expression of genes associated with mitochondrial signaling impairment. Our data show that the expression of PGC-1α, cytochrome c, MFN-2, and Drp-1 was deregulated. Mitochondrial-signaling impairment was further validated by redox-system dysregulation, which showed a significant increase in ROS and thiobarbituric acid reactive substances, both in serum and heart tissue, whereas the superoxide dismutase, catalase, and glutathione levels were decreased. Additionally, the expression levels of pro-apoptotic gene PUMA and stress marker GATA-4 genes were elevated, whereas ARC, PPARα, and Bcl-2 expression levels were decreased in the heart tissues of diabetic rats. Importantly, these alloxan-induced impairments were rescued by N-acetyl cysteine, ascorbic acid, and selenium treatment. This was demonstrated by the amelioration of myocardial stiffness, fibrosis, mitochondrial gene expression, lipid profile, restoration of myocyte size, reduced oxidative stress, and the activation of enzymes associated with antioxidant activities. Altogether, these data indicate that the improvement of mitochondrial dysfunction by protective agents such as N-acetyl cysteine, selenium, and ascorbic acid could rescue diabetes-associated cardiac complications, including DCH.

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Designing a Silymarin Nanopercolating System Using CME@ZIF-8: An Approach to Hepatic Injuries

Yu,

Saif,

Hasan

et al. 2023

ACS Omega

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It is commonly known that silymarin, a phytoconstituent obtained from the Silybum marianum plant, has hepatoprotective and antioxidative properties. However, its low oral bioavailability and poor water solubility negatively impact its therapeutic efficacy. The goal of the present study was to determine the efficiency of the Cordia myxa extract-based synthesized zeolitic imidazole metal–organic framework (CME@ZIF-8 MOF) for increasing silymarin’s bioavailability. A coprecipitation technique was used to synthesize the CME@ZIF-8 and polyethylene glycol-coated silymarin-loaded MOFs (PEG-Sily@CME@ZIF-8) and a complete factorial design was used to optimize them. The crystalline size of CME@ZIF-8 was 14.7 nm and the size of PEG-Sily@CME@ZIF-8 was 17.39 nm. The loading percentage of the silymarin drug in CME@ZIF-8 was 33.5%. The optimized formulations were then characterized by ultraviolet–visible (UV–vis) spectroscopy, X-ray diffraction, Fourier transform IR spectroscopy, surface morphology, gas chromatography–mass spectrometry, and drug release in an in vitro medium. Additionally, a rat model was used to investigate the optimized formulation’s in vivo hepatoprotective effectiveness. The synthesized silymarin-loaded CME@ZIF-8 MOFs were distinct particles with a porous, spongelike shape and a diameter of (size) nm. Furthermore, the designed silymarin-loaded PEG-Sily@CME@ZIF-8 MOF formulation exhibited considerable silymarin release from the synthesized formula in dissolution investigations. The in vivo evaluation studies demonstrated that the prepared PEG-Sily@CME@ZIF-8 MOFs effectively exhibited a hepatoprotective effect in comparison with free silymarin in a CCl4-based induced-hepatotoxicity rat model via ameliorating the normal antioxidant enzyme levels and restoring the cellular abnormalities produced by CCl4 toxication. In combination, biologically produced CME@ZIF-8 may promise to be a viable biologically based nanocarrier that can enhance the loading and release of silymarin medication, which has low solubility in water.

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Indigofera linifolia ameliorated CCl4 induced endoplasmic reticulum stress in liver of rat

Cited by 4 publications

References 54 publications

Pleurospermum candollei Methanolic Extract Ameliorates CCl₄-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats

Pleurospermum candollei Methanolic Extract Ameliorates CCl₄-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats

N-Acetyl Cysteine, Selenium, and Ascorbic Acid Rescue Diabetic Cardiac Hypertrophy via Mitochondrial-Associated Redox Regulators

Designing a Silymarin Nanopercolating System Using CME@ZIF-8: An Approach to Hepatic Injuries

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Indigofera linifolia ameliorated CCl4 induced endoplasmic reticulum stress in liver of rat

Cited by 4 publications

References 54 publications

Pleurospermum candollei Methanolic Extract Ameliorates CCl4-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats

Pleurospermum candollei Methanolic Extract Ameliorates CCl4-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats

N-Acetyl Cysteine, Selenium, and Ascorbic Acid Rescue Diabetic Cardiac Hypertrophy via Mitochondrial-Associated Redox Regulators

Designing a Silymarin Nanopercolating System Using CME@ZIF-8: An Approach to Hepatic Injuries

Contact Info

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Resources

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Pleurospermum candollei Methanolic Extract Ameliorates CCl₄-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats

Pleurospermum candollei Methanolic Extract Ameliorates CCl₄-Induced Liver Injury by Modulating Oxidative Stress, Inflammatory, and Apoptotic Markers in Rats