Indigenous proteases in the skin of unicorn leatherjacket (Alutherus monoceros) and their influence on characteristic and functional properties of gelatin

Ahmad, Mehraj; Benjakul, Soottawat; Ovissipour, Mahmoudreza; Prodpran, Thummanoon

doi:10.1016/j.foodchem.2011.01.032

Cited by 40 publications

(19 citation statements)

References 33 publications

(46 reference statements)

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“…On the basis of the inhibitor study, indigenous proteases in the skin of unicorn leatherjacket were classified as serine protease (Ahmad et al . ). When thermal hydrolysis was implemented, the yield of gelatin hydrolysate with heating time of 60 min (TH60) increased, compared with those of TH15 and TH30 ( P < 0.05).…”

Section: Resultsmentioning

confidence: 97%

“…Removal of noncollagenous proteins was carried out according to the method of Ahmad et al . (), with a slight modification. Fish skins (0.5 × 0.5 cm 2 ) were soaked in 0.05 M NaOH with a skin/alkaline solution ratio of 1:10 (w/v) to remove noncollagenous proteins.…”

Section: Methodsmentioning

confidence: 99%

“…Skin of unicorn leatherjacket ( Aluterus monoceros ) has been reported to possess indigenous protease, which caused the degradation of α‐chain during gelatin extraction at 50–55C (Ahmad et al . ; Kaewruang et al . ).…”

Section: Introductionmentioning

confidence: 99%

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Characteristics and Antioxidative Activity of Gelatin Hydrolysates from Unicorn Leatherjacket Skin as Affected by Autolysis-Assisted Process

Karnjanapratum

Benjakul

2014

Journal of Food Processing and Preservation

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Autolysis-assisted process mediated by indigenous protease in combination with thermal hydrolysis and hydrolysis using papain was used for production of gelatin hydrolysate from skin of unicorn leatherjacket. Prior autolysis could enhance the yield and antioxidative activity of gelatin hydrolysates, especially when subsequent hydrolysis by 2% papain was implemented (AU + HP2.0). Hydrolysates possessed ABTS (2,2′-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) radical and H2O2 scavenging activities as well as metal chelating activity. AU + HP2.0 and gelatin hydrolysate prepared using papain without prior autolysis (HP2.0) could retard oxidation in lecithin liposome system, and antioxidative activity of hydrolysates at 10.0 g/L was comparable to that of 0.1 g/L Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), in which the oxidation was almost completely inhibited. On the basis of size exclusion chromatography, antioxidative peptide in AU + HP2.0 had molecular weight of 2200 Da. Therefore, autolysisassisted process showed the potential for the production of gelatin hydrolysates with enhanced antioxidative activity. PRACTICAL APPLICATIONSThe skin of unicorn leatherjacket contains heat-activated protease, which is capable of hydrolyzing gelatin. Autolysis-assisted process mediated by indigenous protease increased the yield and enhanced the antioxidative activity of gelatin hydrolysates from the skin. Moreover, gelatin hydrolysate can be produced directly from fish skin without prior gelatin extraction. The autolysis-assisted process could be promising for production of gelatin hydrolysate with antioxidative activity, in which the amount of external or commercial proteases could be less required and the indigenous protease was fully exploited.

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Section: Resultsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

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Characteristics and Antioxidative Activity of Gelatin Hydrolysates from Unicorn Leatherjacket Skin as Affected by Autolysis-Assisted Process

Karnjanapratum

Benjakul

2014

Journal of Food Processing and Preservation

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“…This is also true for the case of N. septentrionalis . Its skin is reported to be composed of high surface‐energy organics such as collagen, explaining the underwater oleophobicty via its chemical composition . Furthermore, the closely arrayed hook‐like spines increase the performance of underwater oleophobicty by tightly interlocking plenty of water molecules into the spaces of each spines.…”

Section: Resultsmentioning

confidence: 99%

Filefish‐Inspired Surface Design for Anisotropic Underwater Oleophobicity

Cai

Lin

Zhang

et al. 2013

Adv Funct Materials

231

187

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Surfaces with anisotropic wettability, widely found in nature, have inspired the development of one-dimensional water control on surfaces relying on the well-arranged surface features. Controlling the wetting behavior of organic liquids, especially the motion of oil fl uid on surfaces, is of great importance for a broad range of applications including oil transportation, oil-repellent coatings, and water/oil separation. However, anisotropic oil-wetting surfaces remain unexplored. Here, the unique skin of a fi lefi sh Navodon septentrionalis shows anisotropic oleophobicity under water. On the rough skin of N. septentrionalis , oil droplets tend to roll off in a head-to-tail direction, but pin in the opposite direction. This pronounced wetting anisotropy results from the oriented hook-like spines arrayed on the fi sh skin. It inspires further exploration of the artifi cial anisotropic underwater oleophobic surfaces: By mimicking the oriented hook-like microstructure on a polydimethylsiloxane layer via soft lithography and subsequent oxygen-plasma treatment to make the PDMS hydrophilic, artifi cial fi sh skin is fabricated which has similar anisotropic underwater oleophobicity. Drawn from the processing of artifi cial fi sh skin, a simple principle is proposed to achieve anisotropic underwater oleophobicity by adjusting the hydrophilicity of surface composition and the anisotropic microtextures. This principle can guide the simple mass manufacturing of various inexpensive high surface-energy materials, and the principle is demonstrated on commercial cloth corduroy. This study will profi t broad applications involving low-energy, low-expense oil transportation, underwater oil collection, and oil-repellant coatings on ship hulls and oil pipelines.

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“…The application of autolysis reactions to prepare oligopeptides has advantages of its simplicity and inexpensive costs [12]. Peptides have been obtained by autolysis reaction from mackerel meat [13], unicorn leatherjacket skin [14] and shrimp head [15]. Usually, enzymatic hydrolysis is an effective approach to recover oligopeptides with antioxidant activity from marine foods, including vertebrate and invertebrate processing and unwanted parts, for example, grass carp muscle [16], oyster meat [17], sea urchin gonad [18] and hoki skin [19].…”

Section: Introductionmentioning

confidence: 99%

Identification of antioxidative oligopeptides derived from autolysis hydrolysates of sea cucumber (Stichopus japonicus) guts

Zheng

Zhu

et al. 2012

Eur Food Res Technol

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Oligopeptides were prepared from the guts of sea cucumber Stichopus japonicus by autolysis method. Optimum autolysis conditions for preparing oligopeptides from the guts of sea cucumber were determined by response surface methodology using a central composite rotatable design. The effects of two independent variables, namely temperature and pH, on the response of trichloroacetic acid-soluble oligopeptides (mg/g on dry basis) were investigated. Regression analysis indicated that more than 95 % of the variation could be explained by the fitted models. Temperature at 48.30°C and pH at 4.43 were found to be the optimal conditions to obtain oligopeptides from the guts of sea cucumber. The autolysis hydrolysates prepared at the optimized conditions were further fractionated into four major fractions (I-IV) by size exclusion chromatography on a Sephadex G-15 column. Fraction IV, which exhibited the highest DPPH radical scavenging capacity, Fe 2? -chelating ability and protective effect against hydroxyl radical-induced DNA damage, was then analyzed by ESI-MS for molecular mass determination and ESI-MS/MS for the characterization of peptides. Two tetrapeptides (Val-Thr-Pro-Tyr and Val-Leu-Leu-Tyr) and a hexapeptide (Val-Gly-Thr-Val-Glu-Met) were found to exhibit protective effects against hydroxyl radical-induced DNA damage. These results suggest that antioxidant oligopeptides derived from the guts of sea cucumber by autolysis method could be utilized for functional foods.

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Indigenous proteases in the skin of unicorn leatherjacket (Alutherus monoceros) and their influence on characteristic and functional properties of gelatin

Cited by 40 publications

References 33 publications

Characteristics and Antioxidative Activity of Gelatin Hydrolysates from Unicorn Leatherjacket Skin as Affected by Autolysis-Assisted Process

Characteristics and Antioxidative Activity of Gelatin Hydrolysates from Unicorn Leatherjacket Skin as Affected by Autolysis-Assisted Process

Filefish‐Inspired Surface Design for Anisotropic Underwater Oleophobicity

Identification of antioxidative oligopeptides derived from autolysis hydrolysates of sea cucumber (Stichopus japonicus) guts

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