Indigenous bacterial consortium-mediated cypermethrin degradation in the presence of organic amendments and Zea mays plants

Bhatt, Pankaj; Rene, Eldon R.; Huang, Yaohua; Wu, Xiaozhen; Zhou, Zhe; Li, Jiayi; Kumar, Alagarasan Jagadeesh; Sharma, Anita; Chen, Shaohua

doi:10.1016/j.envres.2022.113137

Cited by 51 publications

(18 citation statements)

References 75 publications

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“…The internal morphology of the immobilized gel beads was conducted by a scanning electron microscope (SEM). The middle sections of immobilized gel beads were hand cut and washed three times in sodium phosphate buffer, and then fixed in 2.5% (v/v) glutaraldehyde for 12 h at 4 °C . After fixation, samples were dehydrated in a dilution series of ethanol and dried in a draught cupboard.…”

Section: Methodsmentioning

confidence: 99%

“…30 Various materials are emerging as skeletons for tailoring microbial immobilization, such as silkworm excrement, sodium alginate, cereal straw, farmyard manure, press mud compost, fresh cow dung, and gypsum. 31,32 Ha et al 33 reported an immobilized consortium consisting of chlorferon-degrading bacteria that provided 5-times-higher degradation than the freely suspended cells. However, whether the cell immobilization technique can be effectively applied to glyphosatedegrading bacteria remains unknown.…”

Section: Introductionmentioning

confidence: 99%

“…The bacterial cell immobilization technique, using natural or synthetic materials to restrict the high density of degraders to a fixed space, provides bacteria a protected environment in which to survive, slows the microbial dispersal speed, exhibits improved removal efficiency of target compounds, and is reusable . Various materials are emerging as skeletons for tailoring microbial immobilization, such as silkworm excrement, sodium alginate, cereal straw, farmyard manure, press mud compost, fresh cow dung, and gypsum. , Ha et al reported an immobilized consortium consisting of chlorferon-degrading bacteria that provided 5-times-higher degradation than the freely suspended cells. However, whether the cell immobilization technique can be effectively applied to glyphosate-degrading bacteria remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Free or Immobilized Bacterium Stenotrophomonas acidaminiphila Y4B on Glyphosate Degradation Performance and Indigenous Microbial Community Structure

Chen

Zhang

et al. 2022

J. Agric. Food Chem.

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The overuse of glyphosate has resulted in serious environmental contamination. Thus, effective techniques to remove glyphosate from the environment are required. Herein, we isolated a novel strain Stenotrophomonas acidaminiphila Y4B, which completely degraded glyphosate and its major metabolite aminomethylphosphonic acid (AMPA). Y4B degraded glyphosate over a broad concentration range (50−800 mg L −1 ), with a degradation efficiency of over 98% within 72 h (50 mg L −1 ). Y4B degraded glyphosate via the AMPA pathway by cleaving the C−N bond, followed by degradation of AMPA and subsequent metabolism. Y4B demonstrated strong competitiveness and substantially accelerated the degradation of glyphosate in different soils, degrading 71.93 and 89.81% of glyphosate (400 mg kg −1 ) within 5 days in sterile and nonsterile soils, respectively. The immobilized cells of Y4B were more efficient than their free cells and they displayed excellent biodegradation efficiency in a sediment−water system. Taken together, Y4B is an ideal degrader for the bioremediation of glyphosate-contaminated sites.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Free or Immobilized Bacterium Stenotrophomonas acidaminiphila Y4B on Glyphosate Degradation Performance and Indigenous Microbial Community Structure

Chen

Zhang

et al. 2022

J. Agric. Food Chem.

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“…6). The results obtained could be compared with the studies that have studied microbial communities combined with plants, those studies indicated that the removal of cypermethrin is better in the presence of plants ( Bhatt et. al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Establishing the Catabolic Pathway of Commercial Cypermethrin and Discovering the Inhibitor of 3- Phenoxy Benzoic Acid Biodegradation by Fig Farm Isolated Enterobacter hormaechei strain ZK101 and Stenotrophomonas maltophilia strain ZK102

Kurt

KOKANGUL

2023

Preprint

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Cypermethrin (CYP) is a commonly used type Ⅱ pyrethroid against pest control in agriculture and household. Its excessive use has the potential to cause serious secondary environmental pollution. Biodegradation in the environment minimizes contamination however the metabolites, could also harm the environment more than the main product of interest. Therefore, optimum degradation rates and possible effects of bioremediation should be understood to evaluate its applicability. In this study, isolates obtained from soil exposed to CYP from fig farms in Adana were investigated to identify their CYP biodegradation pathway, and the key enzymes responsible for biodegradation, to optimize the biodegradation, and to indicate the inhibitors that affect their ability to mineralize CYP. Enterobacter hormaechei strain ZK101 and Stenotrophomonas maltophilia strain ZK102 both degraded CYP with first-order kinetics through esterification followed by 3-phenoxy benzoic acid (3PBA) deoxygenation. Strains accumulated 3PBA when they have been reinoculated to the soil due to the copper present (40 ppm) in the soil causing the inhibition of 3PBA dioxygenase. The work here is the first study that tested the potential of CYP degradation when isolates were re-inoculated in the soil and showed that CYP degraders in the field will cause an accumulation of a more toxic contaminant than CYP. The results of this study help to understand the effects of bacterial applications for CYP contamination and reveal the negative effects of microbial responses in the field.

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“…The controls contained only atrazine without bacterial inoculation. The degradation efficiency of atrazine by immobilized bacterial mixture and bacterial suspensions was calculated using the degradation efficiency of atrazine of the control as the baseline (Bhatt et al, 2022). Then, the Box-Behnken module of the design-Expert.V8.0.6 was used to design the experiments with the three factors (i.e., temperature, pH level, and initial concentration of atrazine) affecting the degradation of atrazine by IM-FN optimized for response surface analysis (Supplementary Table 2).…”

Section: Atrazine Degradation Assaysmentioning

confidence: 99%

Immobilization of bacterial mixture of Klebsiella variicola FH-1 and Arthrobacter sp. NJ-1 enhances the bioremediation of atrazine-polluted soil environments

Pan

Zhai

et al. 2023

Front. Microbiol.

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In this study, the effects of the immobilized bacterial mixture (IM-FN) of Arthrobacter sp. NJ-1 and Klebsiella variicola strain FH-1 using sodium alginate-CaCl2 on the degradation of atrazine were investigated. The results showed that the optimal ratio of three types of carrier materials (i.e., rice straw powder, rice husk, and wheat bran) was 1:1:1 with the highest adsorption capacity for atrazine (i.e., 3774.47 mg/kg) obtained at 30°C. On day 9, the degradation efficiency of atrazine (50 mg/L) reached 98.23% with cell concentration of 1.6 × 108 cfu/ml at pH 9 and 30°C. The Box–Behnken method was used to further optimize the culture conditions for the degradation of atrazine by the immobilized bacterial mixture. The IM-FN could be reused for 2–3 times with the degradation efficiency of atrazine maintained at 73.0% after being stored for 80 days at 25°C. The population dynamics of IM-FN was explored with the total soil DNA samples specifically analyzed by real-time PCR. In 7 days, the copy numbers of both PydC and estD genes in the IM-FN were significantly higher than those of bacterial suspensions in the soil. Compared with bacterial suspensions, the IM-FN significantly accelerated the degradation of atrazine (20 mg/kg) in soil with the half-life shortened from 19.80 to 7.96 days. The plant heights of two atrazine-sensitive crops (wheat and soybean) were increased by 14.99 and 64.74%, respectively, in the soil restored by immobilized bacterial mixture, indicating that the IM-FN significantly reduced the phytotoxicity of atrazine on the plants. Our study evidently demonstrated that the IM-FN could significantly increase the degradation of atrazine, providing a potentially effective bioremediation technique for the treatment of atrazine-polluted soil environment and providing experimental support for the wide application of immobilized microorganism technology in agriculture.

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Indigenous bacterial consortium-mediated cypermethrin degradation in the presence of organic amendments and Zea mays plants

Cited by 51 publications

References 75 publications

Effects of Free or Immobilized Bacterium Stenotrophomonas acidaminiphila Y4B on Glyphosate Degradation Performance and Indigenous Microbial Community Structure

Effects of Free or Immobilized Bacterium Stenotrophomonas acidaminiphila Y4B on Glyphosate Degradation Performance and Indigenous Microbial Community Structure

Establishing the Catabolic Pathway of Commercial Cypermethrin and Discovering the Inhibitor of 3- Phenoxy Benzoic Acid Biodegradation by Fig Farm Isolated Enterobacter hormaechei strain ZK101 and Stenotrophomonas maltophilia strain ZK102

Immobilization of bacterial mixture of Klebsiella variicola FH-1 and Arthrobacter sp. NJ-1 enhances the bioremediation of atrazine-polluted soil environments

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