ABSTRACT.-We proposed a numerical index for evaluating human heat tolerance which is practically useful. The adaptive changes in heat tolerance of unacclimatized subjects during short-term heat acclimatization could be evaluated by this index. The present investigation was performed to examine whether or not our index can reflect effects on heat tolerance of a long-term acclimatization as seen in subtropical natives. Nineteen young male residents born and raised in Okinawa (subtropical zone), 18 male residents born in the Japan Main Islands (temperate zone) but moved to Okinawa and 25 male students in Kyoto (temperate zone) were chosen to be subjects. Their age were from 19 to 29 years. Sweating reaction was examined for 60 rain in summer, by immersing legs in stirring water of 42°C by using a mobile climatic chamber of 30oc with 70 RH. Local sweat samples from the chest and back were collected at 15 rain interval by the filter paper method. Sweating reaction of the residents born and raised in Okinawa was characterized by smaller volume of sweating and lower salt concentration in sweat, while the rise in rectal temperature and increase in heart rate differ less between the three groups. It is concluded that residents born and raised in Okinawa showed higher heat tolerance than the other two groups when assessed by our factorial index method.