Indicators of resistance of sunflower plant to basal stalk rot (Sclerotinia sclerotiorum): Symptomatological, biochemical, anatomical, and morphological characters of the host

Bazzalo, M. E.; Dimarco, P.; Martínez, Fabián; Daleo, Gustavo Raúl

doi:10.1007/bf00039666

Cited by 18 publications

(13 citation statements)

References 12 publications

(13 reference statements)

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“…It is possible than the higher phenolic content in this organ due to the presence of flavonoid pigments mask differences in minority antifungal compounds. Our data confirm previously published results on phenolic induction in Helianthus wild species and cultivated sunflower vegetative tissue (leaves and stem) and in response to Sclerotinia infection (Bazzalo et al, 1985;1986;1991;Mondolot-Cosson et al, 1997;Tourvieille et al, 1997 ). 5 ii)…”

Section: Discussionsupporting

confidence: 92%

“…As chemical control is not practical, genetic control through the development of resistant lines appears to be the best crop protection strategy. Immunity to the fungus has not yet been found, although a wide range of susceptibility to attacks under field conditions has been described among 15 sunflower inbred lines, varieties and hybrids (Gulya, 1985;Bazzalo et al, 1991). Accordingly, a major goal of research is to identify compounds involved in resistance, in order to be used as molecular markers in plant breeding programmes or to design appropriate control strategies.…”

Section: Introductionmentioning

confidence: 99%

“…The accumulation of soluble phenolic compounds, their deposition on cell walls, melanization and lignification are typical sunflower responses to Sclerotinia infection which takes place in stem and leaves in both infected and healthy surrounding tissue, this pathogen-induced accumulation being, in general, lower in 10 susceptible Sclerotinia varieties (Bazzalo et al, 1985(Bazzalo et al, , 1987Hemery-Tardin et al, 1998). In a previous paper, Bazzalo et al (1991), present data of relationships between the field reactions of eight sunflower-inbred lines to the basal stalk rot and phenolic concentration in healthy tissue around the lesion zones. A strong negative correlation between postinfectional, but not preinfectional, phenolic accumulation 15 and the wilting range (stalk rot) for individual plants was obtained, although a clear interpretation of these results in terms of genetic resistance is difficult due to the great influence of environmental factors on the production of phenolic compounds (Jorrín & Prats, 1999).…”

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confidence: 99%

“…In previous studies with vegetative tissue of wild Helianthus spp. and cultivated sunflower such a correlationship has been found between postinfectional but not preinfectional levels (Bazzalo et al, 1991) or it have been difficult to establish (Tourvieille et al, 1997). These results are very promising as it could be possible to use specific phenolic compounds directly as biochemical markers or 10 through associated molecular markers in plant breeding programmes for resistance to Sclerotinia.…”

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confidence: 99%

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Prats

Bazzalo²,

Leόn³

et al. 2003

Euphytica

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Summary 5Disease symptoms and total soluble phenolics content have been analysed in four sunflower (Helianthus annuus L.) lines with different resistance levels (from highly susceptible to resistant) to head rot caused by Sclerotinia sclerotiorum (Lib.) de Bary. 10At the beginning of the flowering stage, capitula were inoculated by spraying with a water suspension of ascospores, and disease symptoms were evaluated from day 6 to day 14 after inoculation. The most susceptible genotypes showed all their ovaries to be necrosed and abundant lesions in corollas, bracts and receptacle. In the resistant line, the ovary and corolla werer only partially necrosed with no symptoms 15 in the bracts or the receptacle.Total soluble phenolics were extracted and quantified from different parts of the capitulum in both inoculated and non-inoculated plants. The amount of phenolic compounds depended on the sunflower line, the time after inoculation, and the 20 tissue. Higher constitutive and induced phenolic content as well as phenylalanine ammonia-lyase activity were present in the most resistant line, these differences being correlated with the absence/presence of disease symptoms.3

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Prats

Bazzalo²,

Leόn³

et al. 2003

Euphytica

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“…Different features, related to the resistance mechanisms of the plant could be applied for screening the host genotypes (Bazzalo et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Sclerotinia-Induced Accumulation of Protein in the Basal Stem of Resistant and Susceptible Lines of Sunflower

Davar

Darvishzadeh

Majd³

2012

Not Bot Hort Agrobot Cluj

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Sunflower, Helianthus annuus L. is a major oilseed crop widely cultivated across the globe. White mold, caused by the necrotrophic pathogen Sclerotinia sclerotiorum (Lib.) de Bary, is a common and widespread pathogen of sunflower. Changes in a partially resistant and a susceptible sunflower line infected with Sclerotinia sclerotiorum were studied 12, 24 and 48 h after inoculation. Electrophoretic patterns and quantitative changes in soluble proteins were determined in the basal stem region. Soluble proteins were accumulated post infection in the partially resistant line. A rapid accumulation of stress-related, low molecular weight proteins was induced in both lines by different pathways. By 12 and 24 h post inoculation, stress proteins with molecular masses of 27 kDa had accumulated in infected stems of the partially resistant line. SDS-PAGE results showed the accumulation of proteins with a molecular mass of 55 kDa in the susceptible line and the absence of this band in the resistant line. This relatively faster response to Sclerotinia sclerotiorum invasion could be partially responsible for the resistance or susceptibility to this pathogen. The differences between lines may also indicate further avenues worth exploring in host-pathogen relations which could ultimately lead to selection and production of new lines with higher levels of resistance to Sclerotinia sclerotiorum.

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Resistance of sunflower inbred lines to various forms of attack by Sclerotinia sclerotiorum and relations with some morphological characters

1993

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Twenty sunflower inbred lines were studied for their reactions to 7 Sclerotinia sclerotiorum tests on different plant parts. A principal component analysis (EC.A.) and t-tests on the means of resistant and susceptible groups indicated that reactions of inbred lines to infection of capitula by ascospores are independent from those to ascospore infections of terminal buds. They are even more contrasted with the results of any test measuring mycelial extension. However, for the last, there is a close association between the reactions of roots, leaves and capitula. Of the inbred lines, some showed good levels of resistance to most forms of attack, others were generally susceptible and there were some with good resistance to one particular form of attack. A EC.A. of 18 morphological and field characters showed no general association between these characters and Sclerotinia sclerotiorurn test results, although it was found that lines resistant to mycelial extension on capitula were generally earlier than those that were more susceptible. It is proposed that breeding programmes for general resistance to Sclerotinia sclerotiorum should include a combination of two or three tests.

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Indicators of resistance of sunflower plant to basal stalk rot (Sclerotinia sclerotiorum): Symptomatological, biochemical, anatomical, and morphological characters of the host

Cited by 18 publications

References 12 publications

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Sclerotinia-Induced Accumulation of Protein in the Basal Stem of Resistant and Susceptible Lines of Sunflower

Resistance of sunflower inbred lines to various forms of attack by Sclerotinia sclerotiorum and relations with some morphological characters

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