INDIANA: An in-cell diffusion method to characterize the size, abundance and permeability of cells

Karunanithy, Gogulan; Wheeler, Richard J.; Tear, Louise R.; Farrer, Nicola J.; Faulkner, Stephen; Baldwin, Andrew J.

doi:10.1016/j.jmr.2018.12.001

Cited by 16 publications

(25 citation statements)

References 57 publications

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“…For yeast's ≈ 4 µm spherical diameter 57 , ā is calculated as 42 and 21 µm at these cell densities, suggesting contributions from sub-cellular length scales. This ensemble ā estimate of ≃ 2 µm (≡ V /S ≃ 3 µm) is within the range of estimates (ā ∼ 1 − 5 µm) reported in previous PFG and SG DW-NMR studies of similar yeast densities [57][58][59][60][61] . These yeast results are considered non-quantitative, however, due to T 2 contributions, the Gaussian phase approximation, and the stationary ĝT R(t, t ′ )ĝ assumption.…”

Section: Resultssupporting

confidence: 88%

A single-shot measurement of time-dependent diffusion over sub-millisecond timescales using static field gradient NMR

Cai,

Williamson,

Witherspoon

et al. 2020

Preprint

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Time-dependent diffusion behavior is probed over sub-millisecond timescales in a single shot using an NMR static gradient, time-incremented echo train acquisition (SG-TIETA) framework. The method extends the Carr-Purcell-Meiboom-Gill (CPMG) cycle under a static field gradient by discretely incrementing the πpulse spacings to simultaneously avoid off-resonance effects and probe a range of timescales (50 − 500 µs). Pulse spacings are optimized based on a derived ruleset. The remaining effects of pulse inaccuracy are examined and found to be consistent across pure liquids of different diffusivities: water, decane, and octanol-1. A pulse accuracy correction is developed. Instantaneous diffusivity, D inst (t), curves (i.e., half of the time derivative of the mean-squared displacement in the gradient direction), are recovered from pulse accuracycorrected SG-TIETA decays using a model-free, log-linear least squares inversion method validated by Monte Carlo simulations. A signal-averaged, 1-minute experiment is described. A flat D inst (t) is measured on pure dodecamethylcyclohexasiloxane whereas decreasing D inst (t) are measured on yeast suspensions, consistent with the expected short-time D inst (t) behavior for confining microstructural barriers on the order of microns.

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Section: Resultssupporting

confidence: 88%

A single-shot measurement of time-dependent diffusion over sub-millisecond timescales using static field gradient NMR

Cai,

Williamson,

Witherspoon

et al. 2020

Preprint

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“…The PGSTE NMR experiment is sensitive to translational motion over length scales of micrometers; therefore, these results indicate not only that the two reservoirs do not mix or mix slowly but also that water moves over at least hundreds of nanometers within each of the reservoirs without exchanging . Time-dependent diffusion NMR experiments, , recorded with different Δ, are also consistent with two coupled water reservoirs in the slow exchange limit in N-HTC, which is not seen in HTC (Figure S5).…”

Section: Resultsmentioning

confidence: 54%

Insights into Water Interaction at the Interface of Nitrogen-Functionalized Hydrothermal Carbons

et al. 2019

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Hydrothermal carbon (HTC) derived from biomass is a class of cost-efficient, eco-friendly functional carbon materials with various potential applications. In this work, solidstate nuclear magnetic resonance (NMR), longitudinal (T 1 ) relaxation time, and diffusion NMR were employed to investigate the structure and water dynamics for HTC and nitrogenfunctionalized hydrothermal carbon (N-HTC) samples ((N)-HTC). Results showed that the presence of N-functional groups influences the water interaction with (N)-HTC more strongly than surface area, pore size distribution, or oxygenated functional groups. Furthermore, the degree of water interaction can be tuned by adjusting the synthesis temperature and the precursor ratio. Water motion was more strongly inhibited in N-HTC than in N-free HTC, thereby suggesting the existence of a differently structured hydration shell around N-HTC particles. In addition, the diffusion data of water in the N-HTC material show two components that do not exchange on the time scale of the experiment (tens of milliseconds), indicating a significant fraction of slow mobile water that exists inside the structure of N-HTC. 1 H− 2 H isotope exchange and cross-polarization NMR results show this internal water only in a near-surface layer of the N-HTC particles. Based on these findings, a model for water interaction with (N)-HTC particles is proposed.

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“…As shown previously, using the INDIANA model, 16 the diffusive behaviour of water within cellular systems can be robustly described using a two-pool model with the water residing in either an intra- or extra-cellular pool. This results from the intrinsic diffusion of water being slower in the viscous intra-cellular compartment and its maximum displacement being restricted by the presence of the cell membrane.…”

Section: Resultsmentioning

confidence: 97%

“…14,15 To enable us to localise gadolinium compounds, we recently developed a diffusion-based 1 H NMR method that can be used to distinguish between intra-and extracellular pools of water, relying on the fact that water diffusion in intracellular fluids is defined by the boundaries of the cell. 16 Since the cell is much smaller than the voxel (volume pixel) in an MRI image, this means that intracellular fluid can be treated as a slow diffusing pool, whilst extra-cellular fluid is treated as a fast diffusing pool. The INDIANA (IN cell DIffusion Analysis) methodology allows these two pools and their properties to be quantitatively described, including relaxation rates.…”

Section: Introductionmentioning

confidence: 99%

Cell-permeable lanthanide–platinum(iv) anti-cancer prodrugs

et al. 2021

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INDIANA: An in-cell diffusion method to characterize the size, abundance and permeability of cells

Cited by 16 publications

References 57 publications

A single-shot measurement of time-dependent diffusion over sub-millisecond timescales using static field gradient NMR

A single-shot measurement of time-dependent diffusion over sub-millisecond timescales using static field gradient NMR

Insights into Water Interaction at the Interface of Nitrogen-Functionalized Hydrothermal Carbons

Cell-permeable lanthanide–platinum(iv) anti-cancer prodrugs

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