Indian medicinal plants as a source of antimycobacterial agents

Gautam, Raju; Saklani, Arvind; Jachak, Sanjay M.

doi:10.1016/j.jep.2006.12.031

Cited by 252 publications

(176 citation statements)

References 93 publications

Supporting

Mentioning

162

Contrasting

Unclassified

Order By: Relevance

“…India represented by its rich flora of medicinal plants, tradition and natural biodiversity, offers a unique opportunity for drug discovery researchers (Gautam et al 2007). This country is well recognized for its heritage of the world's most ancient traditional system of medicine, Ayurveda (Gautam et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

et al. 2015

View full text Add to dashboard Cite

Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor activity. Extract/fractions were prepared and in vitro cytotoxicity was assessed using SRB assay. Most effective fractions were subjected to fluorescence microscopy based acridine orange/ethidium bromide (AO/EB) and Hoechst 33342 staining to determine apoptosis induction and DNA fragmentation assay. Comet and micronuclei assay were performed to assess genotoxicity. Cell cycle analysis was also performed. In vivo anti-tumor potential was evaluated by Ehrlich ascites carcinoma (EAC) model in mice. The alcoholic stem bark extract of M. elengi along with four fractions showed potential in vitro cytotoxicity in SRB assay. Of these, dichloromethane and ethyl acetate fractions were selected for further studies. The fractions revealed apoptosis inducing potential in AO/EB and Hoechst 33342 staining, which was further confirmed by DNA fragmentation assay. Genotoxic potential was revealed by comet and micronuclei assay. Fractions also exhibited specific cell cycle inhibition in G 0 /G 1 phase. In EAC model, ethyl acetate fraction along with the standard (cisplatin) effectively reduced the increase in body weight compared to control and improved mean survival time. Both fractions were able to restore the altered hematological and biochemical parameters. Hence, M. elengi stem bark may be a possible therapeutic candidate having cytotoxic and anti-tumor potential.

show abstract

Section: Introductionmentioning

confidence: 99%

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

et al. 2015

View full text Add to dashboard Cite

show abstract

“…There were many different reference to analyze the antituberculosis activity of plant extracts. In previous study, plants extract were considered active against M. tuberculosis if MIC was <100µg/mL (Borges-Argáez et al, 2007;Gautam et al, 2007;Okunade et al, 2004), <200µg/mL (Camacho-Corona et al, 2008;Tosun et al, 2004), < 1600µg/mL (Mohamad et al, 2011), ≤ 2048 µg/ml (Tekwu et al, 2012). In other paper, the antimicrobial activity of plant extracts was classified as significant (MIC ≤100µg/mL), moderate (100 < MIC < 625µg/mL), or weak (MIC > 625 µg/mL) (Kuete, 2010).…”

Section: Resultsmentioning

confidence: 99%

Antituberculosis Activity of Brotowali (Tinospora crispa) Extract and Fractions against Mycobacterium tuberculosis using Microplate Alamar Blue Assay Method

et al. 2017

View full text Add to dashboard Cite

Tuberculosis (TB), in which caused by pathogenic bacteria, Mycobacterium tuberculosis, has become the major causes of death among all of infectious diseases. The increasing incidence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB ABSTRAK Tuberkulosis (TB) adalah penyakit yang disebabkan oleh infeksi Mycobacterium tuberculosis dan menjadi penyakit infeksi yang menyebabkan paling banyak kematian di dunia. Daruratnya penyakit TB dan meningkatnya kasus multidrug-resistant tuberculosis (MDR-TB) dan XDR-TB ini mendorong dilakukannya

show abstract

“…The plants used for treatment of diseases are medicinal plants. The medicinal plants utilization in medicine was first reported in Ayurveda of Indian traditional medicine and Chinese traditional medicine [4][5][6] . Herbal medicines derived from plant extracts are being increasing utilized to treat a wide variety of diseases, though relatively little knowledge about their mode of action available because of their less side effects and low cost 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Anti-Tubercular Activity of Oxystelma Esculantum

Srikanth¹,

Prasadarao²,

Badrinath³

et al. 2017

Int. Res. J. Pharm.

View full text Add to dashboard Cite

Oxystelma esculentum is one of the traditional medicinal plant which have been using in treatment of different diseases with less scientific evidence belongs to the family Ascelepiadaceae. In this point of view, the present work carried out to evaluate its anti-tuberculosis, which is the most mortality causing diseases around the world. The qualitative phytochemical analysis was carried out by standard protocols and anti-tuberculosis activity was carried out using Microplate Alamar Blue Assay method on Mycobacterium tuberculosis reference strain H37Rv. The ethanolic extract of O. esculentum flowers showed the positive results for the sterols, phenols, alkaloids, flavonoids, glycosides, tannins and carbohydrates and gave negative result for terpenoids, saponins and oils. The MIC of the extracts which showed inhibitory effect on M. tuberculosis H37Rv was determined at various concentrations of 1, 6.125, 12.5, 25, 50, 100 mg/ml. Accordingly, the MIC of Ethanolic and Aqueous extract O. esculentum in ethanol and water of were 1 mg/ml and 1 mg/ml respectively. So, the present study supports its medicinal u se and further studies on isolation of particular bioactive compounds from O. esculentum are useful.

show abstract

Indian medicinal plants as a source of antimycobacterial agents

Cited by 252 publications

References 93 publications

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

Antituberculosis Activity of Brotowali (Tinospora crispa) Extract and Fractions against Mycobacterium tuberculosis using Microplate Alamar Blue Assay Method

Anti-Tubercular Activity of Oxystelma Esculantum

Contact Info

Product

Resources

About