In this paper, we study the nonperiodic first-order Hamiltonian systemu = JL(t)u + JH (t, u), where H ∈ C 1 (R × R 2n ). With some assumptions on L, the corresponding Hamiltonian operator has only discrete spectrum. By using the index theory for self-adjoint operator equation, we establish the existence of multiple homoclinic orbits for the asymptotically quadratic nonlinearty satisfying some twist conditions between infinity and origin.