“…de Bary is an important pathogen, which infects more than 400 different plant species worldwide (Boland & Hall, 1994). In soybean [Glycine max (L.) Merr.]…”
-The objective of this work was to evaluate the genetic diversity among Sclerotinia sclerotiorum isolates from Brazil and the USA, assess their aggressiveness variability, and verify the existence of an isolate-cultivar interaction. Isolate variability was determined by mycelial compatibility grouping (MCG), and isolate aggressiveness by cut-stem inoculations of soybean cultivars. Two experiments for MCGs and two for aggressiveness were conducted with two sets of isolates. The first set included nine isolates from the same soybean field in Brazil and nine from the Midwest region of the USA. The second set included 16 isolates from several regions of Brazil and one from the USA. In the first set, 18 isolates formed 12 different MCGs. In the second set, 81% of the isolates from Brazil grouped into a single MCG. No common MCGs were observed among isolates from Brazil and the USA. The isolates showed aggressiveness differences in the first set, but not in the second. Although aggressiveness differed in the first set, soybean cultivars and isolates did not interact significantly. Cultivar rank remained the same, regardless of the genetic diversity, aggressiveness difference, and region or country of origin of the isolate. Results from screening of soybean cultivars, performed by the cut-stem method in the USA, can be used as reference for researchers in Brazil.Index terms: Glycine max, Sclerotinia stem rot, disease resistance, screening of cultivars, white mold. Termos para indexação: Glycine max, podridão-de-esclerotinia, resistência a doenças, seleção de cultivares, mofo-branco.
Compatibilidade micelial e agressividade de isolados de Sclerotinia sclerotiorum do Brasil e dos Estados Unidos
“…de Bary is an important pathogen, which infects more than 400 different plant species worldwide (Boland & Hall, 1994). In soybean [Glycine max (L.) Merr.]…”
-The objective of this work was to evaluate the genetic diversity among Sclerotinia sclerotiorum isolates from Brazil and the USA, assess their aggressiveness variability, and verify the existence of an isolate-cultivar interaction. Isolate variability was determined by mycelial compatibility grouping (MCG), and isolate aggressiveness by cut-stem inoculations of soybean cultivars. Two experiments for MCGs and two for aggressiveness were conducted with two sets of isolates. The first set included nine isolates from the same soybean field in Brazil and nine from the Midwest region of the USA. The second set included 16 isolates from several regions of Brazil and one from the USA. In the first set, 18 isolates formed 12 different MCGs. In the second set, 81% of the isolates from Brazil grouped into a single MCG. No common MCGs were observed among isolates from Brazil and the USA. The isolates showed aggressiveness differences in the first set, but not in the second. Although aggressiveness differed in the first set, soybean cultivars and isolates did not interact significantly. Cultivar rank remained the same, regardless of the genetic diversity, aggressiveness difference, and region or country of origin of the isolate. Results from screening of soybean cultivars, performed by the cut-stem method in the USA, can be used as reference for researchers in Brazil.Index terms: Glycine max, Sclerotinia stem rot, disease resistance, screening of cultivars, white mold. Termos para indexação: Glycine max, podridão-de-esclerotinia, resistência a doenças, seleção de cultivares, mofo-branco.
Compatibilidade micelial e agressividade de isolados de Sclerotinia sclerotiorum do Brasil e dos Estados Unidos
“…This disease leads to significant losses in seed quantity and quality (Zhao et al 2004). Sclerotinia sclerotiorum is one of the non-specific plant pathogens (Purdy 1979) and attacks over 360 plant species in 61 families (Boland & Hall 1994;Paul 2003). In the Czech Republic this disease attacks many agronomic field crops such as potatoes, oilseed rape, poppy, sunflower, and various vegetable crops (Spitzer et al 2012).…”
Poslušná J. Plachká E., Mazáková J. (2018): Influence of selected fungicides registered in the Czech Republic for winter oilseed rape on in vitro Sclerotinia sclerotiorum mycelial growth. Plant Protect. Sci., 54: 101-110.The baseline sensitivity of 55 isolates of Sclerotinia sclerotiorum, collected from oilseed rape in 6 regions of the Czech Republic, to selected fungicides was determined during the period 2013-2015. One single-component fungicide -Horizon (tebuconazole), and four multicomponent fungicides -Pictor (boscalid, dimoxystrobin), Efilor (boscalid, metconazole), Prosaro 250 EC (prothioconazole, tebuconazole), and Propulse (fluopyram, prothioconazole), were chosen as these are commonly used locally. The effect of each fungicide on the in vitro pathogen radial mycelial growth and EC 50 values for the respective fungicides were determined. The following MIC values were estimated; for the fungicides Horizon 250 EW, Efilor, and Propulse the mean MIC values ranged between 0.125 and 0.250 µl/ml, for Prosaro 250 EC ranged between 0.0625 and 0.125 µl/ml, and for Pictor ranged from 0.00781 to 0.01562 µl/ml. No strains of S. sclerotiorum resistant to the tested fungicides were detected and the growth of all isolates was fully inhibited at concentrations corresponding to their registered dose rates. The highest fungicidal efficacy on the collected S. sclerotiorum isolates was recorded for Pictor, followed by Prosaro 250 with an EC 50 value 0.05856 µl/ml and then the remaining fungicides Propulse, Efilor, and Horizon 250 EW (EC 50 values 0.07277, 0.07221, and 0.08519 µl/ml, respectively).
“…Sclerotinia sclerotiorum is a polyphagous and aggressive fungus; it hosts over 408 species of plants such as soybean, sunflower, canola, peas, beans, alfalfa, tobacco, tomato and potato (BOLAND;HALL, 1994). On soybean, the disease is known as white mold and it can cause damage of up to 70%, as found by Jaccoud Filho et al (2010a) in the state of Paraná, Brazil.…”
ABSTRACT:The objective of this study was to verify the efficiency of the control of white mold on soybean with the use of fungicides applied alone and in rotation, at different growth stages and in a mixture of two active ingredients at three locations: Arapoti, PR, Mauá-da-Serra, PR and Pinhão, PR, Brazil. The fungicides used were carbendazim (Ca), thiophanate methyl (Tm), procymidone (Pr) and fluazinam (Fl). The experiments consisted on 17 treatments and 4 replications in a randomized block design. The analyzed variables were severity, incidence, number of sclerotia and yield. Mauá-da-Serra and Pinhão presented the highest incidences (31% and 29.8% in the control, respectively). At these two locations most of treatments with fungicides decreased the incidence and production of sclerotia, when compared to control; however, no differences in terms of yield were observed. Arapoti presented the lowest incidence (15.8% in the control) where most of treatments with fungicides did not present differences for the variables incidence, production of sclerotia and yield, when compared to the control. No differences were also observed for severity in any of three locations. In conclusion, fungicides applied in soybean areas with historically white mold incidence up to 31% can reduce the disease incidence and sclerotia production levels.
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