Independent Vesicle Pools Underlie Different Modes of Release during Neuronal Development

Andreae, Laura C.; Fredj, Naila Ben; Burrone, Juan

doi:10.1523/jneurosci.5181-11.2012

Cited by 53 publications

(61 citation statements)

References 26 publications

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“…We show that β-catenin mRNA is locally translated and that this translation regulates synaptic vesicle dynamics. How local β-catenin translation is regulated remains unknown, although the absence of postsynaptic targets in our model system suggests an autonomous presynaptic signal (Andreae et al, 2012). Recent evidence supports the involvement of target-derived signals including netrin and NT3 in the local translation of β-catenin in thalamic axons (Pratt et al, 2012) and during the initial outgrowth of hippocampal axons (Kundel et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Proper brain development requires that long projecting axons form presynaptic boutons quickly and with little involvement from the somatic compartment (McAllister, 2007; Andreae et al, 2012). Evidence from Aplysia and rat hippocampal neurons supports a role for local translation in regulating functional bouton assembly (Schacher and Wu, 2002; Lyles et al, 2006; Sebeo et al, 2009), providing a rapid and spatially restricted mechanism to nucleate synaptic vesicle release sites.…”

Section: Introductionmentioning

confidence: 99%

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Axonal Translation of β-Catenin Regulates Synaptic Vesicle Dynamics

et al. 2013

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Many presynaptic transcripts have been observed in axons, yet their role in synapse development remains unknown. Using visually and pharmacologically isolated presynaptic terminals from dissociated rat hippocampal neurons, we found that ribosomes and β-catenin mRNA preferentially localize to recently formed boutons. Locally translated β-catenin accumulates at presynaptic terminals where it regulates synaptic vesicle release dynamics. Thus, local translation of β-catenin is a newly described mechanism for axons to independently functionalize nerve terminals at great distances from cellular somata.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Axonal Translation of β-Catenin Regulates Synaptic Vesicle Dynamics

et al. 2013

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“…The spontaneously and activity-dependent recycling pools are differentially sensitive to phorbol ester regulation 20 , 21 as well as dynamin inhibition 22 . Divergence in the vesicle populations released at rest or with stimulation has also been observed in GABAergic terminals 23 as well as throughout neuronal development and synaptic maturation 24 , 25 . Furthermore, vesicles released under different forms of neurotransmission may undergo mechanistically different fusion reactions 26 , 27 .…”

Section: Introductionmentioning

confidence: 99%

The role of non-canonical SNAREs in synaptic vesicle recycling

Ramirez¹,

Kavalali²

2012

Cellular Logistics

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An increasing number of studies suggest that distinct pools of synaptic vesicles drive specific forms of neurotransmission. Interspersed with these functional studies are analyses of the synaptic vesicle proteome which have consistently detected the presence of so-called “non-canonical” SNAREs that typically function in fusion and trafficking of other subcellular structures within the neuron. The recent identification of certain non-canonical vesicular SNAREs driving spontaneous (e.g., VAMP7 and vti1a) or evoked asynchronous (e.g., VAMP4) release integrates and corroborates existing data from functional and proteomic studies and implies that at least some complement of non-canonical SNAREs resident on synaptic vesicles function in neurotransmission. Here, we discuss the specific roles in neurotransmission of proteins homologous to each member of the classical neuronal SNARE complex consisting of synaptobrevin2, syntaxin-1 and SNAP-25.

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“…We performed the shRNA transfection on 16 div neurons, whereas Koo et al transfected neurons at 6–8 div, a much younger culture age (Koo et al 2011). Cultured hippocampal neurons that are younger than 10 div, although they already possess SVs, undergo mostly spontaneous neurotransmitter release; only when neurons become more mature (older than 10 div), do they switch to evoked neurotransmitter release (Andreae et al 2012). It is conceivable that the requirement for SV recycling changes according to the mode of neurotransmitter release.…”

Section: Discussionmentioning

confidence: 99%

Reduction of AP180 and CALM Produces Defects in Synaptic Vesicle Size and Density

Petralia

Wang

Indig³

et al. 2012

Neuromol Med

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Clathrin assembly proteins AP180 and CALM regulate the assembly of clathrin-coated vesicles (CCVs), which mediate diverse intracellular trafficking processes, including synaptic vesicle (SV) recycling at the synapse. Although studies using several invertebrate model systems have indicated a role for AP180 in SV recycling, less is known about AP180’s or CALM’s function in the synapse of mammalian neurons. In this study, we examined synapses of rat hippocampal neurons in which the level of AP180 or CALM had been reduced by RNA interference (RNAi). Using light microscopy, we visualized synaptic puncta in these AP180- or CALM-reduced neurons by co-expressing Synaptophysin::EGFP (Syp::EGFP). We found that neurons with reduced AP180 or reduced CALM had smaller Syp::EGFP-illuminated puncta. Using electron microscopy, we further examined the ultrastructure of the AP180- or CALM-reduced presynaptic terminals. We found that SVs became variably enlarged in both the AP180-reduced and CALM-reduced presynaptic terminals. Lower AP180 and CALM also reduced the density of SVs and the size of SV clusters. Our findings demonstrate that in the presynaptic terminals of hippocampal neurons, AP180 and CALM have a similar role in regulating synaptic vesicles. This overlapping activity may be necessary for high-precision and high-efficacy SV formation during endocytosis.

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Independent Vesicle Pools Underlie Different Modes of Release during Neuronal Development

Cited by 53 publications

References 26 publications

Axonal Translation of β-Catenin Regulates Synaptic Vesicle Dynamics

Axonal Translation of β-Catenin Regulates Synaptic Vesicle Dynamics

The role of non-canonical SNAREs in synaptic vesicle recycling

Reduction of AP180 and CALM Produces Defects in Synaptic Vesicle Size and Density

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