Ind represses <i>msh</i> expression in the intermediate column of the <i>Drosophila</i> neuroectoderm, through direct interaction with upstream regulatory DNA

Ohlen, Tonia Von; Moses, Cade; Poulson, Will

doi:10.1002/dvdy.22096

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…Deletions encompassing either AE1 or AE2 or the AE2 point mutation greatly reduced ME-lacZ expression ( Fig. 3B,C,E ), while deletion of the 3′ most region containing a previously reported Ind site [25] resulted in ventral expansion of reporter gene expression as expected. We tested the possibility that activation of the ME via AE2 might be balanced against repression mediated by the SE1 and SE2 sites by constructing a triple mutant in which all three sites were eliminated.…”

Section: Resultssupporting

confidence: 82%

“…A 700 bp msh CRM (henceforth referred to as ME for Msh Element) has been identified that is directly repressed by Ind [25] . The response of the ME to BMP-mediated regulation has not yet been investigated, however.…”

Section: Resultsmentioning

confidence: 99%

“…The 700 bp msh CRM is described in Von Ohlen et al, 2009 [25] . All primers used in this study and the corresponding constructs generated can be found in Table S1 .…”

Section: Methodsmentioning

confidence: 99%

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BMPs Regulate msx Gene Expression in the Dorsal Neuroectoderm of Drosophila and Vertebrates by Distinct Mechanisms

et al. 2014

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In a broad variety of bilaterian species the trunk central nervous system (CNS) derives from three primary rows of neuroblasts. The fates of these neural progenitor cells are determined in part by three conserved transcription factors: vnd/nkx2.2, ind/gsh and msh/msx in Drosophila melanogaster/vertebrates, which are expressed in corresponding non-overlapping patterns along the dorsal-ventral axis. While this conserved suite of “neural identity” gene expression strongly suggests a common ancestral origin for the patterning systems, it is unclear whether the original regulatory mechanisms establishing these patterns have been similarly conserved during evolution. In Drosophila, genetic evidence suggests that Bone Morphogenetic Proteins (BMPs) act in a dosage-dependent fashion to repress expression of neural identity genes. BMPs also play a dose-dependent role in patterning the dorsal and lateral regions of the vertebrate CNS, however, the mechanism by which they achieve such patterning has not yet been clearly established. In this report, we examine the mechanisms by which BMPs act on cis-regulatory modules (CRMs) that control localized expression of the Drosophila msh and zebrafish (Danio rerio) msxB in the dorsal central nervous system (CNS). Our analysis suggests that BMPs act differently in these organisms to regulate similar patterns of gene expression in the neuroectoderm: repressing msh expression in Drosophila, while activating msxB expression in the zebrafish. These findings suggest that the mechanisms by which the BMP gradient patterns the dorsal neuroectoderm have reversed since the divergence of these two ancient lineages.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

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BMPs Regulate msx Gene Expression in the Dorsal Neuroectoderm of Drosophila and Vertebrates by Distinct Mechanisms

et al. 2014

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“…The following fly lines were used: yellow white ( yw ) and arm-GAL4 , Hml-GAL4 , YP1-GAL4 , MS1096-GAL4 , Gmr-GAL4 , Fhk-GAL4 , UAS-Uck2RNAi (w[1118]; P{GD2761}v16719)and UAS-sanRNAi (w[1118]; P{GD7580}v31742). The yw fly line was maintained at Kansas State University and used as a wild type in these experiments (Von Ohlen et al, 2009). The armadillo ( arm ) -Gal fly line (Sanson et al, 1996) was obtained from Joan Hooper (University of Colorado, Anchutz Medical campus, Denver, CO).…”

Section: Methodsmentioning

confidence: 99%

Ehrlichia chaffeensis replication sites in adult Drosophila melanogaster

Drolia

Ohlen

Chapes

2013

International Journal of Medical Microbiology

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Ehrlichia chaffeensis is a Gram-negative, obligate intracellular bacterium which causes the tick-borne disease human monocytic ehrlichiosis. In vertebrates, E. chaffeensis replicates in monocytes and macrophages. However, no clear cell or tissue tropism has been defined in arthropods. Our group identified two host genes that control E. chaffeensis replication and infection in vivo in Drosophila, Uridine cytidine kinase and separation anxiety. Using the UAS-GAL4 RNAi system, we generated F1 flies (UAS-gene of interest RNAi x tissue-GAL4 flies) that have Uck2 or san silenced in ubiquitous or tissue-specific fashion. When Uck2 or san were suppressed in the hemocytes or in the fat body, E. chaffeensis replicated poorly and caused significantly less severe infections. Silencing of these genes in the eyes, wings, or the salivary glands did not impact fly susceptibility or bacterial replication. Our data suggest that in Drosophila, E. chaffeensis replicates within the hemocytes, the insect homolog of mammalian macrophages, and in the fat body, the liver homolog of mammals.

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Phosphorylation of Ind by MAP kinase enhances Ind-dependent transcriptional repression

Moses

Helman

Paroush

et al. 2011

Developmental Biology

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The Drosophila neuroectoderm is initially subdivided into three longitudinal domains that give rise to columns of neuroblasts. This subdivision is coordinately accomplished by the action of the signaling pathways, Dorsal and Epidermal Growth Factor Receptor (EGFR), in conjunction with the homeodomain proteins, Ventral nervous system defective, Intermediate neuroblasts defective (Ind) and Muscle Segment Homeobox. We previously demonstrated that Ind expression is activated in response to the EGFR pathway. Here we show that EGF signaling subsequently mediates the direct phosphorylation of Ind by MAP kinase, which enhances the capacity of Ind to repress target genes, such as achaete. Specifically, we show that reduced EGF signaling results in diminished repression of achaete in the intermediate column, despite the presence of high levels of Ind protein. We also demonstrate that ectopic activation of MAP kinase results in the lateral expansion of the Ind expression domain with a corresponding reduction in achaete expression. This regulation is also dependent on the co-repressor, Dichaete. Our data indicate that EGF signaling, acting through MAP kinase, impinges on multiple aspects of Ind regulatory activity. While it has been often demonstrated that MAP kinase phosphorylation of transcriptional repressors attenuates their repressor activity, here we provide an example of phosphorylation enhancing repressor activity.

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Ind represses msh expression in the intermediate column of the Drosophila neuroectoderm, through direct interaction with upstream regulatory DNA

Cited by 5 publications

References 35 publications

BMPs Regulate msx Gene Expression in the Dorsal Neuroectoderm of Drosophila and Vertebrates by Distinct Mechanisms

BMPs Regulate msx Gene Expression in the Dorsal Neuroectoderm of Drosophila and Vertebrates by Distinct Mechanisms

Ehrlichia chaffeensis replication sites in adult Drosophila melanogaster

Phosphorylation of Ind by MAP kinase enhances Ind-dependent transcriptional repression

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