“…While many studies have looked at the mechanisms of T. hoshinota ’s competitive success, little is known about its population structure and demographic history based on molecular genetics across its range. Most studies on T. hoshinota have focused on its ecology, physiology ( Soong, Yang & Allen Chen, 2009 ; Hirose & Murakami, 2011 ; Wang et al, 2012a ; Wang et al, 2012b ; DeVoogd, Cleary & Dekker, 2013 ; Wang et al, 2015 ; Nozawa, Huang & Hirose, 2016 ; Nugraha, Zamani & Madduppa, 2020 ), distribution ( Elliott et al, 2016a ; Elliott et al, 2016b ; Madduppa et al, 2017 ; Yang et al, 2018 ; Diraviya Raj et al, 2018 ; Ashok et al, 2019 ; Das et al, 2019 ; Thinesh et al, 2015 ), habitat preferences ( Schils, 2012 ; Hsu, Wang & Chen, 2013 ; vander Ent, Hoeksema & de Voogd, 2016 ; Elliott et al, 2016a ; Elliott et al, 2016b ), and interactions between the sponge and other benthic organisms ( Reimer et al, 2011 ; Wang et al, 2012a ; Wang et al, 2012b ; Hoeksema, Waheed & de Voogd, 2014 ; Thinesh et al, 2017a ; Thinesh et al, 2017b ; Syue, Hsu & Soong, 2021 ). vander Ent, Hoeksema & de Voogd (2016) examined the genetic variation of T. hoshinota found within the Spermonde Archipelago, Indonesia, by sequencing partitions of the mitochondrial COI (COI) and nuclear ribosomal 28S gene (28S).…”