Incubation of exogenous fatty acids with lymphocytes. Changes in fatty acid composition and effects on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene

Stubbs, Christopher D.; Tsang, Wing M.; Belin, J; Smith, Anthony D.; Sm, Johnson

doi:10.1021/bi00553a034

Cited by 68 publications

(22 citation statements)

References 64 publications

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“…The fatty acid composition of the phospholipid fraction of freshly prepared rat lymph node lymphocytes ( Table 2) resembled those reported previously for lymphocyte phospholipids (e.g. [6,8,17,31]). The neutral and phospholipid fatty acid compositions reported here also resemble those reported elsewhere for the mouse peritoneal macrophage [28].…”

Section: C3lsupporting

confidence: 84%

“…There have been a number of studies of lymphocyte membrane fluidity after activation [8][9][10][11][12][13][14][15][16]. However, these studies have yielded conflicting results, some finding no change in fluidity [8,9] and others reporting an increase [10][11][12][13][14][15][16]. Some of these studies have measured fluidity during the period immediately after activation [9][10][11][12] whereas others [8,[13][14][15][16] investigated longer-term changes.…”

Section: Introductionmentioning

confidence: 99%

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Incorporation of fatty acids by concanavalin A-stimulated lymphocytes and the effect on fatty acid composition and membrane fluidity

Calder

Yaqoob

Harvey

et al. 1994

Biochemical Journal

208

144

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The fatty acid compositions of the neutral lipid and phospholipid fractions of rat lymph node lymphocytes were characterized. Stimulation of rat lymphocytes with the T-cell mitogen concanavalin A resulted in significant changes in the fatty acid composition of both neutral lipids and phospholipids (a decrease in the proportions of stearic, linoleic and arachidonic acids and an increase in the proportion of oleic acid). Membrane fluidity was measured using nitroxide spin-label e.s.r., and increased during culture with concanavalin A. Culturing the lymphocytes in the absence of mitogen did not affect fatty acid composition or membrane fluidity. The uptake and fate of palmitic, oleic, linoleic and arachidonic acids were studied in detail; there was a timedependent incorporation of each fatty acid into all lipid classes but each fatty acid had a characteristic fate. Palmitic and arachidonic acids were incorporated principally into phospholipids whereas oleic and linoleic acids were incorporated in similar proportions into phospholipids and triacylglycerols. Oleic acid was incorporated mainly into phosphatidylcholine, palmitic and linoleic acids were incorporated equally into phosphatidylcholine and phosphatidylethanolamine, and arachidonic acid was incorporated mainly into phosphatidylethanolamine. Supplementation of the culture medium with particular fatty acids (myristic, palmitic, stearic, oleic, linoleic, a-linolenic, arachi-

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Section: C3lsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Incorporation of fatty acids by concanavalin A-stimulated lymphocytes and the effect on fatty acid composition and membrane fluidity

Calder

Yaqoob

Harvey

et al. 1994

Biochemical Journal

208

144

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“…Consistent with this interpretation, fully saturated DAG species have been shown to be poor in vitro substrates for DAG acyltransferase [46]. Other studies have shown that lymphocytes [47], neutrophils [48] and cultured fibroblasts [49] exposed to oleate or linoleate synthesize substantial amounts of triacylglycerol. Saturated NEFA (palmitate) did not induce triacylglycerol synthesis in these systems.…”

Section: Discussionmentioning

confidence: 76%

Fatty acids modulate protein kinase C activation in porcine vascular smooth muscle cells independently of their effect on de novo diacylglycerol synthesis

Yang

Howard

et al. 2000

Diabetologia

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Diabetes-induced activation of protein kinase C (PKC) has been implicated in the development of chronic vascular complications [1±4]. Vascular cells exposed to high glucose in vitro accumulate diacylglycerol (DAG) and have higher PKC activities [5]. Similar changes have been shown in animal models [6]. A specific PKC inhibitor attenuates vascular dysfunctions in diabetic animal models [7]. Such results strongly implicate DAG/PKC signalling in the pathogenesis of vascular diabetic complications.Although many studies have shown diabetes increases in vascular DAG, the metabolic origin of this DAG and its relation to PKC activation are less apparent. Diabetes clearly increases de novo lipid synthesis by glycerophosphate acylation [5,6]. High glucose concentrations also increase turnover of phos- Diabetologia (2000) Abstract Aims/hypothesis. Diabetes-induced activation of protein kinase C has been associated with the development of vascular complications. Elevated de novo diacylglycerol synthesis has been postulated to underlie this protein kinase C activation. Diabetes also increases the circulating concentrations of non-esterified fatty acids, which are immediate precursors of diacylglycerol through the de novo pathway. We hypothesized that increased fatty acids contribute to de novo diacylglycerol synthesis and activation of protein kinase C in vascular cells. Methods. Primary cultures of porcine carotid smooth muscle cells were exposed to fatty acids, bound to albumin in physiologic ratios. Diacylglycerol and triacylglycerol were measured in extracts of these cells. Protein kinase C activation was measured as membrane translocation with isoform-specific antibodies.Results. Saturated fatty acids caused considerable accumulation of diacylglycerol through de novo synthesis. Unsaturated fatty acids increased triacylglycerol, but not diacylglycerol. Platelet-derived growth factor activated the a, e and z protein kinase C isoforms. Activation of the a and z isoforms was amplified by oleate pretreatment but inhibited by palmitate. In the absence of growth factor stimulation, neither palmitate nor oleate had any effect on the membrane/cytosol distribution of any protein kinase C isoform. Conclusion/interpretation. Saturated fatty acids elicited de novo diacylglycerol synthesis in vascular smooth muscle cells without activating protein kinase C. Effects of fatty acids on protein kinase C activation by platelet-derived growth factor did not correlate with the effects on de novo diacylglycerol synthesis. These results indicate that de novo diacylglycerol synthesis is, by itself, insufficient to activate protein kinase C. [Diabetologia (2000

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“…Experimentally, one way in which the plasma membrane lipid composition can be modulated is by supplementation of the growth medium fatty acids [25,[29][30][31]. Addition of oleic or linoleic acid for about 4 h to the growth medium of neuroblastoma cells (clone Neuro-2A) had dramatic effects on functional plasma membrane properties, such as a depolarization of membrane potential (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…A change in phospholipid composition upon supplementation with stearic acid, oleic acid or linoleic acid has been described in neuroblastoma x glioma hybrid cells [29] and other cell lines [31,32].…”

Section: Introductionmentioning

confidence: 99%

Effect of fatty acids on plasma membrane lipid dynamics and cation permeability in neuroblastoma cells

Boonstra

Nelemans

Feijen

et al. 1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

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In this study the effects of experimental modifications of plasma membrane lipid lateral mobility on the electrical membrane properties and cation transport of mouse neuroblastoma cells, clone Neuro-2A, have been studied. Short-term supplementation of a chemically defined growth medium with oleic acid or linoleic acid resulted in an increase in the lateral mobility of lipids as inferred from fluorescence recovery after photobleaching of the lipid probe 3,3'-dioctadecylindocarbocyanide iodide. These changes were accompanied by a marked depolarization of the membrane potential from -51 mV to -36 mV, 1.5 h after addition, followed by a slow repolarization. Tracer flux studies, using S6Rb÷ as a radioactive tracer for K ÷ , demonstrated that the depolarization was not caused by changes in (Na ÷ + K + )-ATPase-mediated K + influx or in the transmembrane K ÷ gradient. The permeability ratio (PNa/PK), determined from electrophysiological measurements, however, increased from 0.10 to 0.27 upon supplementation with oleic acid or linoleic acid. This transient rise of PN,/Px Was shown by 24Na+ and S6Rb+ flux measurements to be due to both an increase of the Na + permeability and a decrease of the K ÷ permeability. None of these effects occurred upon supplementation of the growth medium with stearic acid.

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Incubation of exogenous fatty acids with lymphocytes. Changes in fatty acid composition and effects on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene

Cited by 68 publications

References 64 publications

Incorporation of fatty acids by concanavalin A-stimulated lymphocytes and the effect on fatty acid composition and membrane fluidity

Incorporation of fatty acids by concanavalin A-stimulated lymphocytes and the effect on fatty acid composition and membrane fluidity

Fatty acids modulate protein kinase C activation in porcine vascular smooth muscle cells independently of their effect on de novo diacylglycerol synthesis

Effect of fatty acids on plasma membrane lipid dynamics and cation permeability in neuroblastoma cells

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