Incremental Value (Bayesian Framework) of Thoracic Ultrasonography over Thoracic Auscultation for Diagnosis of Bronchopneumonia in Preweaned Dairy Calves

Buczinski, Sébastien; Ménard, Jessie; Timsit, Edouard

doi:10.1111/jvim.14361

Cited by 22 publications

(42 citation statements)

References 22 publications

(58 reference statements)

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“…This included a visual assessment of the steer for clinical signs associated with BP, specifically depression, cough, nasal discharge, and ocular discharge. A complete thoracic auscultation was also performed to detect abnormal lung sounds, as described [56]. Furthermore, a thoracic ultrasonography of the cranio-ventral portion of both sides of the thorax was performed to detect lung consolidation (> 1 cm deep) or pleural effusion, as described [57].…”

Section: Methodsmentioning

confidence: 99%

Topography of the respiratory tract bacterial microbiota in cattle

et al. 2020

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Background: Bacterial bronchopneumonia (BP) is the leading cause of morbidity and mortality in cattle. The nasopharynx is generally accepted as the primary source of pathogenic bacteria that cause BP. However, it has recently been shown in humans that the oropharynx may act as the primary reservoir for pathogens that reach the lung. The objective was therefore to describe the bacterial microbiota present along the entire cattle respiratory tract to determine which upper respiratory tract (URT) niches may contribute the most to the composition of the lung microbiota. Methods: Seventeen upper and lower respiratory tract locations were sampled from 15 healthy feedlot steer calves. Samples were collected using a combination of swabs, protected specimen brushes, and saline washes. DNA was extracted from each sample and the 16S rRNA gene (V3-V4) was sequenced. Community composition, alphadiversity, and beta-diversity were compared among sampling locations. Results: Microbiota composition differed across sampling locations, with physiologically and anatomically distinct locations showing different relative abundances of 1137 observed sequence variants (SVs). An analysis of similarities showed that the lung was more similar to the nasopharynx (R-statistic = 0.091) than it was to the oropharynx (Rstatistic = 0.709) or any other URT sampling location. Five distinct metacommunities were identified across all samples after clustering at the genus level using Dirichlet multinomial mixtures. This included a metacommunity found primarily in the lung and nasopharynx that was dominated by Mycoplasma. Further clustering at the SV level showed a shared metacommunity between the lung and nasopharynx that was dominated by Mycoplasma dispar. Other metacommunities found in the nostrils, tonsils, and oral microbiotas were dominated by Moraxella, Fusobacterium, and Streptococcus, respectively. Conclusions: The nasopharyngeal bacterial microbiota is most similar to the lung bacterial microbiota in healthy cattle and therefore may serve as the primary source of bacteria to the lung. This finding indicates that the nasopharynx is likely the most important location that should be targeted when doing bovine respiratory microbiota research.

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Section: Methodsmentioning

confidence: 99%

Topography of the respiratory tract bacterial microbiota in cattle

et al. 2020

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“…This included a visual assessment of the steer for clinical signs associated with BP, specifically depression, cough, nasal discharge, and ocular discharge. A complete thoracic auscultation was also performed to detect abnormal lung sounds, as described [56]. Furthermore, a thoracic ultrasonography of the cranioventral portion of both sides of the thorax was performed to detect lung consolidation (> 1 cm deep) or pleural effusion, as described [57].…”

Section: Methodsmentioning

confidence: 99%

Topography of the respiratory tract bacterial microbiota in cattle

McMullen

Alexander

Léguillette

et al. 2020

Preprint

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BackgroundBacterial bronchopneumonia (BP) is the leading cause of morbidity and mortality in cattle. While the bacterial composition of the bovine upper respiratory tract (URT) has not been studied in detail, the nasopharynx is generally accepted as the primary source of pathogenic bacteria that cause BP.However, it has recently been shown in humans that the oropharynx may act as the primary reservoir for pathogens that reach the lung. The objective was therefore to describe the bacterial microbiota present along the entire cattle respiratory tract to determine which URT niches may contribute the most to the composition of the lung microbiota. MethodsSeventeen upper and lower respiratory tract locations were sampled from 15 healthy feedlot steer calves. Samples were collected using a combination of swabs, protected specimen brushes, and saline washes. DNA was extracted from each sample and the 16S rRNA gene (V3-V4) was sequenced.Community composition, alpha-diversity, and beta-diversity were compared among sampling locations. ResultsMicrobiota composition differed across sampling locations, with physiologically and anatomically distinct locations showing different relative abundances of 1,137 observed sequence variants (SVs).An analysis of similarities showed that the lung was more similar to the nasopharynx (R-statistic = 0.091) than it was to the oropharynx (R-statistic = 0.709) or any other URT sampling location. Five distinct metacommunities were identified across all samples after clustering at the genus level using Dirichlet multinomial mixtures. This included a metacommunity found primarily in the lung and nasopharynx that was dominated by Mycoplasma . Further clustering at the SV level showed a shared metacommunity between the lung and nasopharynx that was dominated by Mycoplasma dispar .Other metacommunities found in the nostrils, tonsils, and oral microbiotas were dominated by Moraxella , Fusobacterium , and Streptococcus , respectively.

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“…Previous work showed that punctual scoring of clinical signs is not accurate enough to confirm pneumonia 5 . Also inter‐rater variability is very large when using scorecards 6 A lot is expected from blood tests and automated detection systems, but to date lung auscultation and thoracic ultrasonography are the most accessible confirmation tests in the field. In the first instance most practitioners will rely on lung auscultation, but its diagnostic accuracy and inter‐rater variability are hardly documented.…”

Section: Figurementioning

confidence: 99%

Accuracy and inter‐rater reliability of lung auscultation by bovine practitioners when compared with ultrasonographic findings

2019

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In practice, veterinary surgeons frequently rely on lung auscultation as a confirmation test for pneumonia. To what extent diagnostic accuracy of lung auscultation varies between different practitioners is currently unknown. In this diagnostic test study, 49 Dutch veterinarians each auscultated between 8 and 10 calves, and communicated whether they would decide to treat the animal with antimicrobials or not. They were not allowed to perform any other aspect of the clinical examination. Their decisions were compared with lung ultrasonography findings. The average sensitivity and specificity of lung auscultation were 0.63 (sd=0.2; range=0.2–1.0) and 0.46 (sd=0.3; range=0.0–1.0), respectively. Of the participants, 8.2 per cent were 100 per cent sensitive, 16.3 per cent were 100 per cent specific, and only 4.0 per cent were perfect. The Krippendorff’s alpha was 0.18 (95 per cent confidence interval: −0.01 to 0.38), signifying poor reliability between multiple raters. Regardless of the poor diagnostic accuracy in this study, especially the large variation in a confirmation test between different practitioners could potentially cause professional damage as well as misuse of antimicrobials. This study could be seen as a gentle stimulus to regularly evaluate one’s diagnostic skills. Both complementary training and the use of more accurate techniques with less inter-rater variation could improve the situation.

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Incremental Value (Bayesian Framework) of Thoracic Ultrasonography over Thoracic Auscultation for Diagnosis of Bronchopneumonia in Preweaned Dairy Calves

Cited by 22 publications

References 22 publications

Topography of the respiratory tract bacterial microbiota in cattle

Topography of the respiratory tract bacterial microbiota in cattle

Topography of the respiratory tract bacterial microbiota in cattle

Accuracy and inter‐rater reliability of lung auscultation by bovine practitioners when compared with ultrasonographic findings

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