“…Consistent with our previously published isogenic DrocA deletion mutant strain transcriptome data, 43 several proven and putative virulence factors had significantly altered transcript levels in the isogenic rocA mutant strains (Supplemental Table S6). Selected virulence factors include the Mga regulon virulence factors 48,49,63 : sclA, 71 fba, 72,73 scpA, 74 enn, 75 emm, 76 mrp, 77 sfbX, 78 and sof (encoding SOF). 79 Other differentially expressed genes are CovRS regulated virulence factors, 29,30 such as nga (encoding SPN), 52 slo (encoding SLO), 52 spyCEP, 80 mac, 81e83 sse, 84 ska (encoding SKA), 85 and speB (encoding SpeB) 54 ( Supplemental Table S6).…”