Increasing Trends in
            <i>mcr-1</i>
            Prevalence among Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates from French Calves despite Decreasing Exposure to Colistin

Haenni, Marisa; Métayer, Véronique; Madec, Jean‐Yves

doi:10.1128/aac.01147-16

Cited by 41 publications

(30 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In agreement with proposal by Petrillo and coauthors [43], the deletion of this IS Apl1 insertion sequence might be the relic of the mcr-1 dissemination. It was reported that the mcr - 1 colistin resistance gene is present in a multidrug-resistant plasmid [22] or coexists with other resistance genes like extended spectrum β-lactamase [16,19–21], and even the notorious NDM-1 [44] and its variants (NDM-5 [23] and NDM-9 [24]). We also found that the pE15017 is an ESBL and MCR-1 coproducing plasmid similar to pA31-12, a recently-isolated plasmid from China [30].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, they observed the paralleled existence of mcr - 1 in meat/food samples and in the healthy human microbiome [11]. Worryingly, the MCR-1 colistin resistance gene was strikingly shown to coexist with other multiple-drug resistance genes (i.e, carbapenem [18] and extended-spectrum β-lactam [16,19–21]), highlighting the possibility that micro-organisms with pan-drug resistances are emerging [22]. For instance, a variant of the notorious NDM-1 was detected to coexist with MCR-1 in the Enterobacteriaceae (NDM-5 in K .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dissemination and Mechanism for the MCR-1 Colistin Resistance

et al. 2016

View full text Add to dashboard Cite

Polymyxins are the last line of defense against lethal infections caused by multidrug resistant Gram-negative pathogens. Very recently, the use of polymyxins has been greatly challenged by the emergence of the plasmid-borne mobile colistin resistance gene (mcr-1). However, the mechanistic aspects of the MCR-1 colistin resistance are still poorly understood. Here we report the comparative genomics of two new mcr-1-harbouring plasmids isolated from the human gut microbiota, highlighting the diversity in plasmid transfer of the mcr-1 gene. Further genetic dissection delineated that both the trans-membrane region and a substrate-binding motif are required for the MCR-1-mediated colistin resistance. The soluble form of the membrane protein MCR-1 was successfully prepared and verified. Phylogenetic analyses revealed that MCR-1 is highly homologous to its counterpart PEA lipid A transferase in Paenibacili, a known producer of polymyxins. The fact that the plasmid-borne MCR-1 is placed in a subclade neighboring the chromosome-encoded colistin-resistant Neisseria LptA (EptA) potentially implies parallel evolutionary paths for the two genes. In conclusion, our finding provids a first glimpse of mechanism for the MCR-1-mediated colistin resistance.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dissemination and Mechanism for the MCR-1 Colistin Resistance

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Its occurrence in animal isolates is quite elevated (232), and it has been identified worldwide. MCR-1-producing E. coli isolates have been identified in several food-producing animals and meat, including chickens and chicken meat, pigs and piglets, cattle, calves, and turkeys (254,255) (Table 4). Those isolates are from many Asian countries (Cambodia, China, Japan, Laos, Malaysia, Taiwan, Singapore, Vietnam, India, Pakistan, South Korea), from Europe (Belgium, Denmark, France, Germany, Portugal, Italy, the Netherlands, Spain, Sweden, Switzerland, the UK), the Americas (Argentina, Brazil, Canada, the U.S., Ecuador, Bolivia, Venezuela), Australia, and Africa (Algeria, Egypt, South Africa, Tunisia).…”

Section: Epidemiology Of Mcr-1mentioning

confidence: 99%

“…Dating the emergence of mcr-1-positive E. coli isolates remains difficult, although a Chinese study retrospectively identified mcr-1-positive isolates from chickens in the 1980s (255) and as early as 2005 in veal calves in France (254). It seems, therefore, that the emergence of mcr-positive isolates, at least in animals, is not a recent event.…”

Section: Epidemiology Of Mcr-1mentioning

confidence: 99%

Antimicrobial Resistance inEscherichia coli

et al. 2018

Self Cite

View full text Add to dashboard Cite

Multidrug resistance in has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic mechanisms in correspond to the acquisition of genes coding for extended-spectrum β-lactamases (conferring resistance to broad-spectrum cephalosporins), carbapenemases (conferring resistance to carbapenems), 16S rRNA methylases (conferring pan-resistance to aminoglycosides), plasmid-mediated quinolone resistance (PMQR) genes (conferring resistance to [fluoro]quinolones), and genes (conferring resistance to polymyxins). Although the spread of carbapenemase genes has been mainly recognized in the human sector but poorly recognized in animals, colistin resistance in seems rather to be related to the use of colistin in veterinary medicine on a global scale. For the other resistance traits, their cross-transfer between the human and animal sectors still remains controversial even though genomic investigations indicate that extended-spectrum β-lactamase producers encountered in animals are distinct from those affecting humans. In addition, of animal origin often also show resistances to other-mostly older-antimicrobial agents, including tetracyclines, phenicols, sulfonamides, trimethoprim, and fosfomycin. Plasmids, especially multiresistance plasmids, but also other mobile genetic elements, such as transposons and gene cassettes in class 1 and class 2 integrons, seem to play a major role in the dissemination of resistance genes. Of note, coselection and persistence of resistances to critically important antimicrobial agents in human medicine also occurs through the massive use of antimicrobial agents in veterinary medicine, such as tetracyclines or sulfonamides, as long as all those determinants are located on the same genetic elements.

show abstract

Section: Epidemiology Of Mcr-1mentioning

confidence: 99%

Antimicrobial Resistance inEscherichia coli

Poirel

Madec

Lupo

et al. 2018

Antimicrobial Resistance in Bacteria From Livestock and Companion Animals

Self Cite

102

View full text Add to dashboard Cite

Multidrug resistance in Escherichia coli has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. E. coli is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic mechanisms in E. coli correspond to the acquisition of genes coding for extended-spectrum β-lactamases (conferring resistance to broad-spectrum cephalosporins), carbapenemases (conferring resistance to carbapenems), 16S rRNA methylases (conferring pan-resistance to aminoglycosides), plasmid-mediated quinolone resistance (PMQR) genes (conferring resistance to [fluoro]quinolones), and mcr genes (conferring resistance to polymyxins). Although the spread of carbapenemase genes has been mainly recognized in the human sector but poorly recognized in animals, colistin resistance in E. coli seems rather to be related to the use of colistin in veterinary medicine on a global scale. For the other resistance traits, their cross-transfer between the human and animal sectors still remains controversial even though genomic investigations indicate that extended-spectrum β-lactamase producers encountered in animals are distinct from those affecting humans. In addition, E. coli of animal origin often also show resistances to other-mostly older-antimicrobial agents, including tetracyclines, phenicols, sulfonamides, trimethoprim, and fosfomycin. Plasmids, especially multiresistance plasmids, but also other mobile genetic elements, such as transposons and gene cassettes in class 1 and class 2 integrons, seem to play a major role in the dissemination of resistance genes. Of note, coselection and persistence of resistances to critically important antimicrobial agents in human medicine also occurs through the massive use of antimicrobial agents in veterinary medicine, such as tetracyclines or sulfonamides, as long as all those determinants are located on the same genetic elements.

show abstract

Increasing Trends in mcr-1 Prevalence among Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates from French Calves despite Decreasing Exposure to Colistin

Cited by 41 publications

References 9 publications

Dissemination and Mechanism for the MCR-1 Colistin Resistance

Dissemination and Mechanism for the MCR-1 Colistin Resistance

Antimicrobial Resistance inEscherichia coli

Antimicrobial Resistance inEscherichia coli

Contact Info

Product

Resources

About