Increasing Threat of Brucellosis to Low-Risk Persons in Urban Settings, China

Chen, Shouyi; Hao, Zhang; Liu, Xiaoning; Wang, Wenjing; Hou, Shuiping; Li, Tingting; Zhao, Shuoxian; Yang, Zhicong; Li, Chengyao

doi:10.3201/eid2001.130324

Cited by 48 publications

(38 citation statements)

References 14 publications

(15 reference statements)

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“…Transmission of Brucella through contaminated milk and milk products is an increasing threat not only for individuals, but also for whole families in urban and rural settings of endemic countries [25]. In these areas, trade of non-pasteurized fresh milk and raw dairy products should be strictly controlled and limited to certified Brucella-free farms.…”

Section: Discussionmentioning

confidence: 99%

Detection of Brucella melitensis in bovine milk and milk products from apparently healthy animals in Egypt by real-time PCR

Wareth

Melzer

Elschner

et al. 2014

J Infect Dev Ctries

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Introduction: Brucellosis in Egypt is an endemic disease among animals and humans. In endemic developing countries, dairy products produced from untreated milk are a potential threat to public health. The aim of this study was to detect brucellae in milk and milk products produced from apparently healthy animals to estimate the prevalence of contamination. Methodology: Two hundred and fifteen unpasteurized milk samples were collected from apparently healthy cattle (n = 72) and buffaloes (n = 128) reared on small farms, and from milk shops (n = 15) producing dairy products for human consumption. All milk samples were examined by indirect enzyme-linked immunosorbent assay (iELISA) and real-time PCR (RT-PCR) to detect Brucella antibodies and Brucella-specific DNA, respectively. Results: Using iELISA, anti-Brucella antibodies were detected in 34 samples (16%), while RT-PCR amplified Brucella-specific DNA from 17 milk samples (7.9%). Species-specific IS711 RT-PCR identified 16 of the RT-PCR-positive samples as containing B. melitensis DNA; 1 RT-PCR-positive sample was identified as containing B. abortus DNA. Conclusions: The detection of Brucella DNA in milk or milk products sold for human consumption, especially the highly pathogenic species B. melitensis, is of obvious concern. The shedding of Brucella spp. in milk poses an increasing threat to consumers in Egypt. Consumption of dairy products produced from non-pasteurized milk by individual farmers operating under poor hygienic conditions represents an unacceptable risk to public health.

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Section: Discussionmentioning

confidence: 99%

Detection of Brucella melitensis in bovine milk and milk products from apparently healthy animals in Egypt by real-time PCR

Wareth

Melzer

Elschner

et al. 2014

J Infect Dev Ctries

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“…bacilli. The cases where there are transmissions through the breast milk of infected mother and human semen have been reported [7]- [11].…”

Section: Discussionmentioning

confidence: 99%

Brucellar Epididymoorchitis: A Report of a Rarely Seen Case at Advanced Age

Bilman¹,

Yetik²,

Çiçek³

2016

OALib

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Although brucellosis is the most common zoonosis all over the world, epididymoorchitis caused by Brucella species is a rarely seen infection. In this report, we present an adult case of epididymoorchitis caused by brucellosis. Patient complained about swollen and painful testicle. Patient had unilateral epididymoorchitis. Brucellosis was diagnosed serologically with lam agglutination test and Coombs Brucella test positivity (1/1280 titer). Patient was treated with rifampicin and doxycycline. In case, complete resolution was achieved with medical treatment and relapse did not occur. Brucellosis should be considered in the differential diagnosis of patients even at old age, presenting with epididymoorchitis in endemic areas.

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“…For instance, B. canis is the causative agent of canine brucellosis, which results in contagious abortion, uveitis, and orchiepididymitis. However, B. canis rarely causes human brucellosis (Chen et al, 2014). Transmission to humans occurs only through close contact with infected animals or bacterial cultures.…”

Section: Introductionmentioning

confidence: 99%

A novel lumazine synthase molecule from Brucella significantly promotes the immune-stimulation effects of antigenic protein

Du¹,

Wang²

2015

Genet. Mol. Res.

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ABSTRACT. Brucella, an intracellular parasite that infects some livestock and humans, can damage or destroy the reproductive system of livestock. The syndrome is referred to as brucellosis and often occurs in pastoral areas; it is contagious from livestock to humans. In this study, the intact Brucella suis outer membrane protein 31 (omp31) gene was cloned, recombinantly expressed, and examined as a subunit vaccine candidate. The intact Brucella lumazine synthase (bls) gene was cloned and recombinantly expressed to study polymerization function in vitro. Non-reducing gel electrophoresis showed that rBs-BLS existed in different forms in vitro, including as a dimer and a pentamer. An enzyme-linked immunosorbent assay result showed that rOmp31 protein could induce production of an antibody in rabbits. However, the rOmp31-BLS fusion protein could elicit a much higher antibody titer in rabbits; this construct involved fusion of the Omp31 molecule with the BLS molecule. Our results indicate that Omp31 is involved in immune stimulation, while BLS has a polymerizing function based on rOmp31-BLS fusion protein immunogenicity. These data 13085 BLS promotes immune-stimulation effects of antigens ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 13084-13095 (2015) suggest that Omp31 is an ideal subunit vaccine candidate and that the BLS molecule is a favorable transport vector for antigenic proteins.

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Increasing Threat of Brucellosis to Low-Risk Persons in Urban Settings, China

Cited by 48 publications

References 14 publications

Detection of Brucella melitensis in bovine milk and milk products from apparently healthy animals in Egypt by real-time PCR

Detection of Brucella melitensis in bovine milk and milk products from apparently healthy animals in Egypt by real-time PCR

Brucellar Epididymoorchitis: A Report of a Rarely Seen Case at Advanced Age

A novel lumazine synthase molecule from Brucella significantly promotes the immune-stimulation effects of antigenic protein

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