Increasing the Viability of Fat Grafts by Vascular Endothelial Growth
                        Factor

Topcu, Alpaslan; Aydin, Osman Enver; Ünlü, Mehtat; Barutçu, Ali; Atabey, Atay

doi:10.1001/archfaci.2011.1633

Cited by 16 publications

(4 citation statements)

References 24 publications

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“…Approaches to overcome poor retention for soft tissue augmentation increase tissue oxygen levels, the survival and number of progenitor cells, and growth factors in the recipient site. The main strategies include physical manipulation of the recipient site (e.g., BRAVA®, microneedling, biostimulation of tissue) [ 6 , 7 ], or injection of either botulinum toxin for facial fat graft [ 8 ], growth factors [ 9 – 11 ], or progenitor cells during the fat graft, i.e., cell-assisted lipotransfer (CAL) [ 12 ]. Supplementation of autologous fat grafts with ASCs [ 13 ], platelet-rich plasma (PRP) [ 14 ], stromal vascular fraction (SVF) [ 15 ], or exosomes [ 16 ] either alone or in combination have increased fat graft retention [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

Ruff,

Sterodimas

2023

Aesth Plast Surg

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Background Improvements to autologous fat grafting for soft tissue augmentation are needed to overcome the unpredictable volume retention. Approaches such as fat harvesting and processing, injection technique, preparation of the recipient site, and supplemental biologics are topics of ongoing research. Here, an energy-based device was investigated as a stimulatory tool for recipient site preparation for improving fat graft retention. Objective The objective was to measure the stimulatory responses in fat grafts after 4 weeks when using a helium-based radiofrequency device to pretreat the recipient tissue. Methods Using an autologous fat grafting mouse model, the inguinal fat pad was grafted in a small cranial pocket after either a saline injection alone (control) or a saline injection followed by pretreatment (treated). The fat pad was resected after 4 weeks, sectioned and stained with immunofluorescence markers to investigate tissue remodeling. Results Pretreatment resulted in higher viability of adipocytes, a higher concentration of viable ASCs in areas of adipose tissue regeneration, and localized macrophages in the areas of regeneration when compared to the control. There was no observable difference in vascularity or angiogenesis. The staining for ASCs was higher in the pretreated group in comparison with the control group (5.0% vs. 3.3%, p=0.36) when using a pixel classifier in QuPath in the viable adipose tissue regions. Conclusions The use of a helium-based radiofrequency device as a pretreatment tool appears to increase the viability of the adipose tissue likely due to higher concentration of ASCs. The apparent increase in viable ASCs may be due to enhanced proliferation or paracrine recruitment of these cells in response to the helium-based radiofrequency treatment. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. Bullet List of Important Points: Pretreatment of the fat graft recipient site increases the viability of the adipose tissue after 4 weeks in comparison with the control grafts. The increased viability is likely due to the observed increase in adipose-derived stem cells in the pretreated group. Pretreatment enhanced the adipose tissue remodeling as colocalization of adipose-derived stem cells and macrophages showed an active remodeling, whereas the control group exhibited more necrotic and fibrotic tissue.

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Section: Introductionmentioning

confidence: 99%

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

Ruff,

Sterodimas

2023

Aesth Plast Surg

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“…Other studies have also demonstrated that ASC-enriched grafts improved fat graft survival through angiogenesis stimulation, 103,109,155–158 and that fat graft enriched in proangiogenic factors improved the graft viability by means of increased vascularization. 159,160 As high-density fat contained more vasculogenic progenitor cells and vascularity cytokines, this fat induces a better fat graft survival compared to low-density fat. 161 These studies show that, through their proangiogenic capacities, ASCs could improve fat graft percentage augmentation.…”

Section: Fat Graft Retentionmentioning

confidence: 99%

Fat Graft Retention: Adipose Tissue, Adipose-Derived Stem Cells, and Aging

Trotzier

Sequeira

Auxenfans

et al. 2022

Plastic &Amp; Reconstructive Surgery

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Over the past 30 years, there has been a dramatic increase in the use of autologous fat grafting for soft-tissue augmentation and to improve facial skin quality. Several studies have highlighted the impact of aging on adipose tissue, leading to a decrease of adipose tissue volume and preadipocyte proliferation and increase of fibrosis. Recently, there has been a rising interest in adipose tissue components, including adipose-derived stem/stromal cells (ASCs) because of their regenerative potential, including inflammation, fibrosis, and vascularization modulation. Because of their differentiation potential and paracrine function, ASCs have been largely used for fat grafting procedures, as they are described to be a key component in fat graft survival. However, many parameters as surgical procedures or adipose tissue biology could change clinical outcomes. Variation on fat grafting methods have led to numerous inconsistent clinical outcomes. Donor-to-donor variation could also be imputed to ASCs, tissue inflammatory state, or tissue origin. In this review, the authors aim to analyze (1) the parameters involved in graft survival, and (2) the effect of aging on adipose tissue components, especially ASCs, that could lead to a decrease of skin regeneration and fat graft retention. (Plast. Reconstr. Surg. 151: 420e, 2023.) Clinical Relevance Statement: This review aims to enlighten surgeons about known parameters that could play a role in fat graft survival. ASCs and their potential mechanism of action in regenerative medicine are more specifically described.

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“…The regained popularity of fat grafting following liposuction has led to a search for various aspects to improve fat graft survival in adipose tissue engineering (ATE). This includes technical aspects of harvesting, processing, and preparation of sterile fat graft [ 14 ] and incorporation of hydrogels or bio factors [ 15 , 16 , 17 ] in order to increase the survivability of fat graft. The technical aspects of harvesting and preparation of fat graft through lipoaspiration have been standardized by Coleman [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Various strategies have been tried to improve fat graft survivability by adding of adipocyte-derived stem cells, scaffolds, hydrogels, and growth factors [ 16 , 17 , 24 ]. The state-of-the-art technology would be isolation, expansion, and seeding of adipose-derived stem cells (ADSCs) on a scaffold for adipose regeneration.…”

Section: Introductionmentioning

confidence: 99%

Injectable Pectin–Alginate Hydrogels for Improving Vascularization and Adipogenesis of Human Fat Graft

Janarthanan

Rangasamy

Iyer

2023

JFB

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Autologous fat grafting (AFG) is the most prevailing tool for soft tissue regeneration in clinics, although efficiency is limited to unpredictable volume resorption due to poor vascularization and eventual necrosis. This study sought to improve the AFG efficiency using a hydrogel as a carrier for human fat graft (F) with and without platelet-rich plasma (PRP). PRP is clinically well known for the local release of several endogenous growth factors and has been in clinical use already. A human-fat-graft-encapsulated pectin–alginate hydrogel (FG) was developed and characterized. PRP was added to F to develop a human fat graft with PRP (FP). FP was admixed with a pectin–alginate hydrogel to develop FGP. FG and FGP showed the smooth injectable, elastic, and shear-thinning properties. FG and FGP groups showed enhanced cell viability and proliferation compared to the control F in vitro. We also investigated the in vivo angiogenesis and neo-adipogenesis ability of F, FG, FGP, and FP in nude mice after subcutaneous injection. After 2 and 4 weeks, an MRI of the mice was conducted, followed by graft explantation. The explanted grafts were also assessed histologically and with immunohistochemistry (IHC) studies. MRI and histology results revealed better vascularity of the FG and FGP system compared to fat graft alone. Further, the IHC studies, CD 31, and perilipin staining also revealed better vasculature and adipogenesis of FG and FGP systems. These results indicate the enhanced angiogenesis and adipogenesis of FG and FGP. Thus, developed pectin–alginate hydrogel-based fat graft systems FG and FGP replenish the native microenvironment by mediating angiogenesis and adipogenesis, thereby maximizing the clinical outcomes of autologous fat grafting.

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Increasing the Viability of Fat Grafts by Vascular Endothelial Growth Factor

Cited by 16 publications

References 24 publications

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

Enhanced Fat Graft Viability and Remodeling Using a Helium-based Radiofrequency Device to Prepare the Recipient Site

Fat Graft Retention: Adipose Tissue, Adipose-Derived Stem Cells, and Aging

Injectable Pectin–Alginate Hydrogels for Improving Vascularization and Adipogenesis of Human Fat Graft

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