“…32,61 For most microorganisms, 35-40 C is the optimum temperature range for growth, enzyme activity, and formation of a stable biolm, thereby increasing the substrate degradation rate and the electricity production in the BES. 29,32,62,63 In many studies, increasing the temperature will increase the power output of the BES. It was demonstrated that the Arrhenius law is followed between anode current output and temperature in the range of 30-45 C. 63 However, when the temperature reaches a certain level, the activity and structure of the microbial enzyme are impaired, thereby reducing the performance of the BES.…”