Increasing the scale of peroxidase production by<i>Streptomyces</i>sp. strain BSII#1

Musengi, Amos; Khan, Nuraan; Roes‐Hill, Marilize Le; Pletschke, Brett I.; Burton, Stephanie G.

doi:10.1111/jam.12380

Cited by 15 publications

(32 citation statements)

References 37 publications

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“…The results showed that the bacterium S. griseosporeus strain SN9 was able to release a maximum peroxidase production yield of 0.172 U/ml at an initial alkaline medium pH (pH 8) after 5 days (Fig. 2E), which is in agreement with other reports on actinomycete-based peroxidase production [7,10]. However, the production of extracellular peroxidase by the S. griseosporeus strain SN9 was significantly decreased to 39% of the maximal production at pH 6.…”

Section: Optimization Of the Production Conditionssupporting

confidence: 93%

“…This contrast with the growth of Streptomyces sp. strain BSII#1 and, hence, peroxidase production reached its highest at 37 • C [7].…”

Section: Optimization Of the Production Conditionsmentioning

confidence: 97%

“…In contrast, the recovery of peroxidase from horseradish roots, for example, is a lengthy and complex procedure involving multiple precipitation, fractionation, chromatography, and filtration steps, with the whole process taking days to weeks, depending on the grade of the enzyme preparation. Furthermore, the process has often been considered as inefficient, with low peroxidase production yields, not exceeding few grams of highest grade peroxidase, being obtained from 1 ton of roots [7]. Antonopoulos et al [8] showed the suitability of an extracellular peroxidase produced by S. albus ATCC 3005 for application in the bleaching of kraft pulps.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

Rekik

Nadia

Bejar

et al. 2015

International Journal of Biological Macromolecules

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Section: Optimization Of the Production Conditionssupporting

confidence: 93%

“…This contrast with the growth of Streptomyces sp. strain BSII#1 and, hence, peroxidase production reached its highest at 37 • C [7].…”

Section: Optimization Of the Production Conditionsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

Rekik

Nadia

Bejar

et al. 2015

International Journal of Biological Macromolecules

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“…The effect of carbon supplementations on extracellular peroxidase activity was assessed using the method of Musengi et al. with slight modifications. Briefly, R. ornithinolytica OKOH‐1 was grown in a KLFM supplemented with 1 mmol L −1 of different lignin model compounds: guaiacol, veratryl alcohol, vanillin, vanillic acid, and ferulic acid at predetermined culture conditions for optimal peroxidase activity (35 °C, pH 5, and 150 rpm).…”

Section: Methodsmentioning

confidence: 99%

“…Given the high‐utility potential of peroxidases, large‐scale production is of utmost importance to the bioeconomy, besides, an effective biocatalytic process requires enhanced enzyme activity and production systems. The production of extracellular peroxidases by some bacteria has been reported . However, most of these reports are on actinomycetes, predominantly Streptomyces species.…”

Section: Introductionmentioning

confidence: 99%

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

Falade

Mabinya

Okoh

et al. 2018

Biotech and App Biochem

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The industrial applications and prospects of microbial peroxidase are on the upwards trend, thus necessitating the search for sources with high turnaround time. Actinobacterial species have been a major source of peroxidase for the obvious reasons of having robust metabolite expression capabilities. However, other bacteria species have been underexplored for peroxidase production, hence the motivation for the investigation into the peroxidase production potential of Raoultella ornithinolytica OKOH-1 (KX640917). The bacteria expressed optimum specific peroxidase activity of 16.48 ± 0.89 U mg −1 , which is higher than those previously reported. The optimal fermentation conditions were pH 5 (3.44 ± 0.64 U mL −1 ), incubation temperature of 35 • C (5.25 ± 0.00 U mL −1 ), and agitation speed of 150 rpm (9.45 ± 2.57 U mL −1 ), with guaiacol and ammonium chloride as the best inducer and nitrogen supplement, respectively. On valorization of agrowastes as a sole carbon source for the secretion of peroxidase, sawdust gave the best peroxidase yield (15.21 ± 2.48 U mg −1 ) under solid-state fermentation. Also, a nonperoxide-dependent enzyme activity, which suggests probable laccase activity, was observed. The ability of the bacteria to utilize agrowastes is highly economical and as well a suitable waste management strategy. Consequently, R. ornithinolytica OKOH-1 is a promising industrial strain with dexterity for enhanced peroxidase production.

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Streptomyces spp. in the biocatalysis toolbox

Spasic

Mandic

Djokić

et al. 2018

Appl Microbiol Biotechnol

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About 20,100 research publications dated 2000-2017 were recovered searching the PubMed and Web of Science databases for Streptomyces, which are the richest known source of bioactive molecules. However, these bacteria with versatile metabolism are powerful suppliers of biocatalytic tools (enzymes) for advanced biotechnological applications such as green chemical transformations and biopharmaceutical and biofuel production. The recent technological advances, especially in DNA sequencing coupled with computational tools for protein functional and structural prediction, and the improved access to microbial diversity enabled the easier access to enzymes and the ability to engineer them to suit a wider range of biotechnological processes. The major driver behind a dramatic increase in the utilization of biocatalysis is sustainable development and the shift toward bioeconomy that will, in accordance to the UN policy agenda "Bioeconomy to 2030," become a global effort in the near future. Streptomyces spp. already play a significant role among industrial microorganisms. The intention of this minireview is to highlight the presence of Streptomyces in the toolbox of biocatalysis and to give an overview of the most important advances in novel biocatalyst discovery and applications. Judging by the steady increase in a number of recent references (228 for the 2000-2017 period), it is clear that biocatalysts from Streptomyces spp. hold promises in terms of valuable properties and applicative industrial potential.

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Increasing the scale of peroxidase production byStreptomycessp. strain BSII#1

Cited by 15 publications

References 37 publications

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

Agrowastes utilization by Raoultella ornithinolytica for optimal extracellular peroxidase activity

Streptomyces spp. in the biocatalysis toolbox

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