Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis

Basile, Natalia; Nogales, M.; Bronet, F.; Florensa, M.; Riqueiros, Marissa; Rodrigo, Lorena; García-Velasco, Juan A.; Meseguer, Marcos

doi:10.1016/j.fertnstert.2013.12.005

Cited by 166 publications

(158 citation statements)

References 33 publications

Supporting

Mentioning

144

Contrasting

Unclassified

Order By: Relevance

“…Our findings suggest that collapsing blastocysts also display suboptimal timing of morphokinetic variables, implying that the underlying common cause is the defective embryo quality, which ultimately manifests itself in a reduced implantation potential. For example, a link between suboptimal morphokinetical timing and aneuploidy rates (and decreased implantation) was already established in previous studies (6,35).…”

Section: Discussionmentioning

confidence: 76%

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Bodri¹,

Sugimoto²,

Serna³

et al. 2016

Fertility and Sterility

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 76%

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Bodri¹,

Sugimoto²,

Serna³

et al. 2016

Fertility and Sterility

View full text Add to dashboard Cite

show abstract

“…syngamy, cleavages, compaction and blastulation), and in a study in published in 2014, we evaluated the possibility to use these parameters and previously published methods to detect aneuploidies and to foresee embryo implantation potential [29]. Differently from previous reports [30,31], no correlation was found between aneuploidy rate and the 16 morphokinetic parameters analysed from ICSI up to completed blastulation. Indeed, the predictive power of time-lapse criteria upon embryo reproductive competence has not been defined yet and they cannot even be considered reliable to define whether a blastocyst should be biopsied or not [32,33].…”

Section: Selection Of the Blastocysts To Undergo Trophectoderm Biopsymentioning

confidence: 99%

Implementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management

Capalbo

Romanelli²,

Cimadomo

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

For an IVF clinic that wishes to implement preimplantation genetic diagnosis for monogenic diseases (PGD) and for aneuploidy testing (PGD-A), a global improvement is required through all the steps of an IVF treatment and patient care. At present, CCS (Comprehensive Chromosome Screening)-based trophectoderm (TE) biopsy has been demonstrated as a safe, accurate and reproducible approach to conduct PGD-A and possibly also PGD from the same biopsy. Key challenges in PGD/PGD-A implementation cover genetic and reproductive counselling, selection of the most efficient approach for blastocyst biopsy as well as of the best performing molecular technique to conduct CCS and monogenic disease analysis. Three different approaches for TE biopsy can be compared. However, among them, the application of TE biopsy approaches, entailing the zona opening when the expanded blastocyst stage is reached, represent the only biopsy methods suited with a totally undisturbed embryo culture strategy (time lapse-based incubation in a single media). Moreover, contemporary CCS technologies show a different spectrum of capabilities and limits that potentially impact the clinical outcomes, the management and the applicability of the PGD-A itself. In general, CCS approaches that avoid the use of whole genome amplification (WGA) can provide higher reliability of results with lower costs and turnaround time of analysis. The future perspectives are focused on the scrupulous and rigorous clinical validations of novel CCS methods based on targeted approaches that avoid the use of WGA, such as targeted next-generation sequencing technology, to further improve the throughput of analysis and the overall cost-effectiveness of PGD/PGD-A.

show abstract

“…Basile et al [25], reported the embryo selection method based on embryo developmental kinetics, ranking the probability of day 3 embryos being chromosomally normal. However, this method has not been validated yet using prospective independent clinical data.…”

Section: Morphokinetics Of the Embryomentioning

confidence: 99%

Novel Scientific Methods and Technology in the Reproductive Medicine

TeresaWiesak¹,

Goryszewska²

2016

Glob J Fertil Res

View full text Add to dashboard Cite

the flexible GnRH antagonist protocol underwent faster with the earliest cleavage, when compared to embryos derived from a woman undergoing the long GnRH agonist protocol. Hence, it is evident that the regiment used for the ovarian stimulation was associated with the embryo cleavage anomaly.Previously, a clear relationship between the time of first cleavage and embryo developmental competence has been demonstrated. The zygotes cleaving earlier after insemination are more likely to reach the blastocyst stage than their later cleaving counterparts [7,8]. This phenomenon is common in many species, e.g. rhesus monkey [9], human [10,11] and buffalo [12]. The factors that control the time of the first cleavage are unclear. However, the gene controlling the rate of preimplantation cleavage divisions and subsequent embryo survival has been identified in mice [13]. Sugimura et al. [14], demonstrated that using multiple predictors such as; timing of the first cleavage, the number of blastomeres at the end of the first cleavage, presence or absence of multiple fragments at the end of the first cleavage, the number of blastomeres at the temporary developmental arrest (lag-phase) during the fourth or fifth cell cycle and oxygen consumption at the blastocyst stage, allows to objectively and reliably select healthy IVF embryos that resulted in a successful pregnancy. It is important for the clinical practice to determine the embryo with the highest potential for implantation and development, as the selective single embryo transfer (eSET) is becoming increasingly popular with a view to reduce the risk for multiple pregnancies effectively. Thus, selecting an optimal embryo for transfer into uterus is a major challenge in assisted reproductive technology. Therefore, novel criteria that will allow evaluate the embryos objectively and reliably are needed to advance the IVF IntroductionBy combining novel scientific and technology accomplishments it is possible to create the-state-of-the-art options for the infertility treatments in humans, and to deliver advancement in animal breeding activities. Embryo creation in vitro with embryo transfer allows to overcome many aspects of human infertility. The most popular criteria to assess the quality of embryo in the clinical and veterinary practice are based on the evaluation of embryo's morphological quality at the time of transfer [1,2]. This approach is extremely subjective and inadequate, because a snapshot morphological assessment of the embryo has a limited success compared to the evaluation of the kinetic changes and embryo morphology over the time of its development [3]. The study of Wong et al. [4], demonstrated that two morphologically similar embryos, being at the same developmental stage at the time of point assessment undergone a completely different developmental process when the kinetics of the embryo was taken into consideration. A recent study of Walls et al. [5], involving the use of time-lapse technology, showed that embryos from hyperandrogenic PCOS women were significa...

show abstract

Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis

Cited by 166 publications

References 33 publications

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Implementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management

Novel Scientific Methods and Technology in the Reproductive Medicine

Contact Info

Product

Resources

About