Increasing the Efficiency of the Accumulation of Recombinant Proteins in Plant Cells: The Role of Transport Signal Peptides

Розов, С. М.; Дейнеко, Е. В.

doi:10.3390/plants11192561

Cited by 4 publications

(3 citation statements)

References 213 publications

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“…The presence of targeting peptides is a critical aspect in recombinant protein design as it determines subcellular compartments and can affect yield [ 2 ]. Here we found that the bacterial protein PmoB, present in methanotroph bacteria, localises to mitochondria when recombinantly expressed in plants.…”

Section: Discussionmentioning

confidence: 99%

“…Plants are an attractive platform for the production of recombinant proteins due to benefits such as cost-effectiveness, ease of scalability and the lack of potential contaminants present in other systems, such as animal viruses or bacterial toxins [ 1 , 2 ]. Targeting proteins to specific compartments within the plant cell is a strategy often utilised to ensure correct functioning of the recombinant system or increase yields.…”

Section: Introductionmentioning

confidence: 99%

“…Targeting proteins to specific compartments within the plant cell is a strategy often utilised to ensure correct functioning of the recombinant system or increase yields. In particular, the apoplast and chloroplast present the lowest protease activity levels, favouring higher yields due to lower transgene degradation [ 2 ]. Retaining or anchoring proteins to the endoplasmic reticulum (ER) has also been reported to increase yields in many cases [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

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An Interplay between Mitochondrial and ER Targeting of a Bacterial Signal Peptide in Plants

Rossi

Kriechbaumer

2023

Plants

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Protein targeting is essential in eukaryotic cells to maintain cell function and organelle identity. Signal peptides are a major type of targeting sequences containing a tripartite structure, which is conserved across all domains in life. They are frequently included in recombinant protein design in plants to increase yields by directing them to the endoplasmic reticulum (ER) or apoplast. The processing of bacterial signal peptides by plant cells is not well understood but could aid in the design of efficient heterologous expression systems. Here we analysed the signal peptide of the enzyme PmoB from methanotrophic bacteria. In plant cells, the PmoB signal peptide targeted proteins to both mitochondria and the ER. This dual localisation was still observed in a mutated version of the signal peptide sequence with enhanced mitochondrial targeting efficiency. Mitochondrial targeting was shown to be dependent on a hydrophobic region involved in transport to the ER. We, therefore, suggest that the dual localisation could be due to an ER-SURF pathway recently characterised in yeast. This work thus sheds light on the processing of bacterial signal peptides by plant cells and proposes a novel pathway for mitochondrial targeting in plants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An Interplay between Mitochondrial and ER Targeting of a Bacterial Signal Peptide in Plants

Rossi

Kriechbaumer

2023

Plants

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The effects of AtEXO70E2 overexpression on the secretion and production of recombinant proteins in tobacco BY-2 suspension cells

Li,

Han,

et al. 2023

In Vitro Cell.Dev.Biol.-Plant

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In‐silico identification of putatively functional intergenic small open reading frames in the cucumber genome and their predicted response to biotic and abiotic stresses

Ahmad,

Abdelsamad,

El‐Shabrawi

et al. 2024

Plant Cell & Environment

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The availability of high‐throughput sequencing technologies increased our understanding of different genomes. However, the genomes of all living organisms still have many unidentified coding sequences. The increased number of missing small open reading frames (sORFs) is due to the length threshold used in most gene identification tools, which is true in the genic and, more importantly and surprisingly, in the intergenic regions. Scanning the cucumber genome intergenic regions revealed 420 723 sORF. We excluded 3850 sORF with similarities to annotated cucumber proteins. To propose the functionality of the remaining 416 873 sORF, we calculated their codon adaptation index (CAI). We found 398 937 novel sORF (nsORF) with CAI ≥ 0.7 that were further used for downstream analysis. Searching against the Rfam database revealed 109 nsORFs similar to multiple RNA families. Using SignalP‐5.0 and NLS, identified 11 592 signal peptides. Five predicted proteins interacting with Meloidogyne incognita and Powdery mildew proteins were selected using published transcriptome data of host‐pathogen interactions. Gene ontology enrichment interpreted the function of those proteins, illustrating that nsORFs' expression could contribute to the cucumber's response to biotic and abiotic stresses. This research highlights the importance of previously overlooked nsORFs in the cucumber genome and provides novel insights into their potential functions.

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Increasing the Efficiency of the Accumulation of Recombinant Proteins in Plant Cells: The Role of Transport Signal Peptides

Cited by 4 publications

References 213 publications

An Interplay between Mitochondrial and ER Targeting of a Bacterial Signal Peptide in Plants

An Interplay between Mitochondrial and ER Targeting of a Bacterial Signal Peptide in Plants

The effects of AtEXO70E2 overexpression on the secretion and production of recombinant proteins in tobacco BY-2 suspension cells

In‐silico identification of putatively functional intergenic small open reading frames in the cucumber genome and their predicted response to biotic and abiotic stresses

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